Chemical Engineering / Kimya Mühendisliği
Permanent URI for this collectionhttps://hdl.handle.net/11147/14
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Article Citation - WoS: 9Citation - Scopus: 10Cvd Deposited Epoxy Copolymers as Protective Coatings for Optical Surfaces(MDPI, 2023) Karabıyık, Merve; Cihanoğlu, Gizem; Ebil, ÖzgençCopolymer thin films of glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA) and 2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane (V4D4) were synthesized via initiated chemical vapor deposition (iCVD) as protective coatings for optical surfaces. Chemical durability in various solvents, corrosion resistance, adhesion to substrate, thermal resistance and optical transmittance of the films were evaluated. Crosslinked thin films exhibited high chemical resistance to strong organic solvents and excellent adhesion to substrates. Poly(GMA-co-EGDMA) and poly(GMA-co-V4D4) copolymers demonstrated protection against water (<1% thickness loss), high salt resistance (<1.5% thickness loss), and high optical transparency (~90% in visible spectrum) making them ideal coating materials for optical surfaces. Combining increased mechanical properties of GMA and chemical durability V4D4, the iCVD process provides a fast and low-cost alternative for the fabrication of protective coatings.Article Citation - WoS: 13Citation - Scopus: 14Detection of Crispr-Cas9 Mutations Using a Carbon Nanotube-Modified Electrochemical Genosensor(MDPI, 2021) Kıvrak, Ezgi; Pauzaite, Tekle; Copeland, Nikki A.; Hardy, John G.; Kara, Pınar; Fırlak, Melike; İnce Yardımcı, Atike; Yılmaz, Selahattin; Palaz, FahreddinThe CRISPR-Cas9 system has facilitated the genetic modification of various model organisms and cell lines. The outcomes of any CRISPR-Cas9 assay should be investigated to ensure/improve the precision of genome engineering. In this study, carbon nanotube-modified disposable pencil graphite electrodes (CNT/PGEs) were used to develop a label-free electrochemical nanogenosensor for the detection of point mutations generated in the genome by using the CRISPR-Cas9 system. Carbodiimide chemistry was used to immobilize the 5 '-aminohexyl-linked inosine-substituted probe on the surface of the sensor. After hybridization between the target sequence and probe at the sensor surface, guanine oxidation signals were monitored using differential pulse voltammetry (DPV). Optimization of the sensitivity of the nanogenoassay resulted in a lower detection limit of 213.7 nM. The nanogenosensor was highly specific for the detection of the precisely edited DNA sequence. This method allows for a rapid and easy investigation of the products of CRISPR-based gene editing and can be further developed to an array system for multiplex detection of different-gene editing outcomes.Article Citation - WoS: 22Citation - Scopus: 23Multi-Organs for Testing Small-Molecule Drugs: Challenges and Perspectives(MDPI, 2021) Çeçen, Berivan; Karavasili, Christina; Nazir, Mubashir; Bhusal, Anant; Doğan, Elvan; Shahriyari, Fatemeh; Tamburacı, Sedef; Miri, Amir K.Organ-on-a-chip technology has been used in testing small-molecule drugs for screening potential therapeutics and regulatory protocols. The technology is expected to boost the development of novel therapies and accelerate the discovery of drug combinations in the coming years. This has led to the development of multi-organ-on-a-chip (MOC) for recapitulating various organs involved in the drug–body interactions. In this review, we discuss the current MOCs used in screening small-molecule drugs and then focus on the dynamic process of drug absorption, distribution, metabolism, and excretion. We also address appropriate materials used for MOCs at low cost and scale-up capacity suitable for high-performance analysis of drugs and commercial high-throughput screening platforms. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.