Phd Degree / Doktora
Permanent URI for this collectionhttps://hdl.handle.net/11147/2869
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Doctoral Thesis Synthesis, Characterization and Investigation of Cytotoxic Effects of Drug Loaded Zif-8 Metal-Organic Frameworks(01. Izmir Institute of Technology, 2021) Mete, Derya; Şanlı Mohamed, Gülşah; Şanlı Mohamed, Gülşah; 01. Izmir Institute of Technology; 04.01. Department of Chemistry; 04. Faculty of ScienceThe biocompatible ZIF-8 intelligent material, a member of the metal-organic framework family, has a biodegradable property in an acidic environment due to its poor coordination bonds. Because cancerous cells are more acidic than healthy cells, our studies aim to ensure that doxorubicin, sorafenib, and apalutamide, encapsulated in ZIF-8, target cancer cells responsive to pH, thereby reducing damage to healthy cells. In addition, ZIF-8 was selected not only as a carrier system but also as a therapeutic effect. Because ZIF-8 material is biodegradable, it is divided into zinc and 2-methylimidazole components in cancer cells. Research shows that the decrease in the amount of zinc is essential in the formation of cancer cells. Zinc is reported to be in lower intracellular concentrations in HCC and prostate cell lines instead of healthy variants. It aims to create dual cytotoxic effects on cancer cells by combining the effects of zinc-drug on a single platform.Doctoral Thesis Synthesis and Characterization of Star Block Copolymer for Controlled Drug Delivery(Izmir Institute of Technology, 2010) Batıgün, Ayşegül; Batıgün, Ayşegül; 03.02. Department of Chemical Engineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyAmphiphilic multiarm block copolymers of hydrophobic poly(methyl methacrylate) core and hydrophilic poly(acrylic acid) corona has been synthesized, characterized and proposed for an anticancer drug that is 5 Florouracil (5FU). 3 arm, 4 arm and 6 arm PMMA-b-PtBA (poly(methyl methacrylate-block-poly(tertiary butyl acrylate)) copolymers with molecular weights from 18 kDa to 80 kDa were synthesized by Atomic Transfer Radical Polymerization and reacted into PMMA-b-PAA (poly(methyl methacrylate-block-poly(acrylic acid)) by hydrolysis of tBA chains. Optimum molecular weight and hydrophobic core ratio was determined by evaluation of critical micelle concentrations and maximum loading capacities with pyrene. Loading method was selected among simple equilibrium, solvent deposition, salting out and dialysis methods. Dialysis method yielded the highest loading contents of model drug indomethacin. Optimum loading conditions in terms of temperature, duration, pH and polymer concentration were determined with anticancer drug 5FU. 4 arm PMMA-b-PAA with molecular weight 18000 Da and hydrophobic core ratio 0.27 was proposed for controlled delivery of 5FU. Optimum loading conditions were determined as 15C in acidic aqueous medium with pH 1.0-1.5 and loading interval as 4 hours. Minimum polymer concentration was estimated to be 2000 mg/L for an optimum loading. Drug loaded particles were characterized by FTIR, TGA, DTG and DSC. 5FU loaded PMMA-b-PAA samples with drug contents about 14-20 % were investigated by a continuous operation where a diffusion cell was employed to monitor release profiles. Controlled release of 5FU with zero order release kinetics for 18 days was provided by 4 arm PMMA-b-PAA. Biodegradation of loaded particles were monitored through particle size analysis by Dynamic Light Scattering and Atomic Force Microscopy.