GCRIS

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Preparation and Characterization of Wound Dressing Contact Layer
(Izmir Institute of Technology, 2015) Erdoğan, İpek; Bayraktar, Oğuz; Atabey, Atay; Başal Bayraktar, Güldemet
Wound dressings provide therapeutic and protective features and promotes natural healing process when applied to a wound area. Being non-toxic and immunologically inert, natural biopolymers have potential in fabrication of wound dressings. Growth factors and antibiotics can also be used in functionalization of wound dressings as well as plant extracts. Olive leaf extract has gained attraction due to its dual antimicrobial and antioxidant effect. By clearing pathogenic microorganisms and scavenging against increased amount of reactive oxygen species in the wound area, it has high potential in wound healing. In this study, olive leaf extract incorporated zein fibers were prepared as a model of wound dressing contact layer. In this regard, crude olive leaf extract was fractionated and characterized in terms of antioxidant capacity, total phenol content and antimicrobial activity. Crude extract and its fractions were also subjected to wound scratch assay in the presence of hydrogen peroxide. Oleuropein, as the most abundant component in crude extract, was found to promote cell migration better and close the wound area at a higher rate than other components. On the other hand, crude olive leaf extract exhibited higher percentage of wound closure than its fractions within the same time period, which may be attributed to synergistic effect of unidentified phenolics. Crude olive leaf extract also provided crosslinking effect when incorporated into zein fibers, as well as promoting cell spreading behaviour.
Investigation of Hemostatic Biomaterials Containing Plant Extracts
(Izmir Institute of Technology, 2014) Uslu, Mehmet Emin; Bayraktar, Oğuz; Başal Bayraktar, Güldemet; Akhisaroğlu, Mustafa
Haemostasis in other words a process which causes bleeding to stop is very important in injury. Recent researches were focused on discovery of haemostatic agents and developing biomaterials which transfer them to the injury side. Therefore plant extracts and three dimensional biomaterials were widely investigated. In this research the extract of Equisetum arvense was investigated as a coagulatory agent. Silk fibroin-hyaluronic acid mixture was used for the preparation of three dimensional sponge like biomaterials. The prepared sponge forms could also be used as scaffold for wound healing. In this research effect of extraction parameters on extract composition and bioactivity was investigated. Effect of extraction parameters were analyzed on 6 factors at 3 levels. It was seen that extraction parameters had high influence on both composition and bioactivity of the prepared extract. Although it was reported that the extract of equisetum arvense had anticoagulant activity, in this research it was shown that changing parameters caused variation of extract bioactivity from anticoagulant to coagulant as a result of changing extract composition. Addition of extract into silk fibroin-hyaluronic acid mixture caused proteins to precipitate as a result of interaction between protein and phenolic compounds. As a result of this precipitation significant decrease in the mechanical strength of biomaterial was observed. In order to minimize this interaction, plant extract was added into the silk fibroin after mixing with hyaluronic acid solution. As a result, mechanical strength and pore size of the biomaterial were increased and pore distribution became more regular. Also biomaterial gained tubular network on both vertical and horizontal dimensions. This would help the proliferation and migration of the fibroblast cells and moreover prevent the formation of scar tissue.

Phd Degree / Doktora

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