Phd Degree / Doktora

Permanent URI for this collectionhttps://hdl.handle.net/11147/2869

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Author: search.filters.author.Emrullahoğlu, MustafaDepartment: search.filters.department.Thesis (Doctoral)--İzmir Institute of Technology, Chemistry

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Now showing 1 - 3 of 3
  • Doctoral Thesis
    Design and Synthesis of Bodipy-Based Palladacycles for the Detection of Carbon Monoxide
    (01. Izmir Institute of Technology, 2024) Eren, Ahmet; Emrullahoğlu, Mustafa
    BODIPY'ler, dikkat çekici ve çok yönlü floresan özellikleriyle takdir edilen önemli bir floresan boya sınıfını temsil eder. Benzersiz kimyasal yapıları nedeniyle, bu boyalar UV-görünür spektrumda ışığı güçlü bir şekilde emer ve daha uzun dalga boylarında floresan yayar. Olağanüstü fotostabiliteleri, elverişli kuantum verimleri, sentez kolaylığı ve çeşitli yapılarda uyarlanabilirlikleri, bilimsel literatürde öne çıkmalarına katkıda bulunur. BODIPY'ler, biyolojik görüntüleme, akış sitometrisi, floresan etiketleme ve tespit dahil olmak üzere kritik uygulamalarda yaygın olarak kullanılır. BODIPY iskeletindeki ağır atomlar, sistemler arası geçiş hızını artırarak floresansı engelleyebilir. Sonuç olarak, kuantum veriminde önemli bir azalma yaşanır ve bu da BODIPY'nin temel floresan özelliğinin kaybolmasına neden olur. Ancak, BODIPY belirli analitler veya moleküllerle özel olarak reaksiyona girme yeteneğine sahiptir ve bu da floresan özelliklerinin geri kazanılmasını sağlar. BODIPY'nin bu ilgi çekici yönü, karbon monoksit gazı için floresan tabanlı dedektörler olarak etkili bir şekilde kullanılan paladyum komplekslerinin sentezine ilham kaynağı olmuştur. Karbon monoksit kokusuz, renksiz ve hem insanlar hem de hayvanlar için oldukça zehirli bir gazdır. Oksijene kıyasla hemoglobine bağlanma afinitesinin belirgin olarak daha yüksek olması nedeniyle tespiti kritik öneme sahiptir. Klinik öncesi çalışmalar ayrıca karbon monoksitin düşük dozlarda uygulandığında anti-inflamatuar etkilere sahip olabileceğini ve canlı organizmalarda hücresel ve doku koruması sağlayabileceğini göstermiştir. Bu çalışmada karbon monoksit, 187 nM gibi çok düşük konsantrasyonlarda bile floresan tekniği kullanılarak tespit edilmiştir. Bu yetenek, floresan mikroskobu kullanılarak canlı insan hücrelerine daha da genişletilmiş ve biyolojik bağlamlarda karbon monoksit tespiti için dikkate değer bir potansiyel olduğu vurgulanmıştır.
  • Doctoral Thesis
    Design and Synthesis of Rhodamine Based Fluorescent and Colorimetric Sensors for the Detection of Gold Ions
    (Izmir Institute of Technology, 2017) Karakuş, Erman; Emrullahoğlu, Mustafa
    The usage of chemosensors for the detection of heavy and transition metal ions is prevalent. Because these metal ions play crucial roles in living systems and have extremely toxic effects on the environment. Among these metal ions, gold species have interesting biological properties and uses. They play important roles in biological systems and often have significant impacts on human health. For instance, gold based drugs have long been used in the treatment of rheumatoid arthritis and other autoimmune diseases. In addition, gold nanoparticles function as carriers for drugs and gene delivery systems. Gold ions, on the other hand, are potentially toxic to humans. Because of their reactive nature, ionic gold species can interact with proteins, DNA and other biomolecules and disturb a series of cellular processes, leading to serious health problems. Detection of gold ions can be performed by spectroscopic methods such as atomic absorption and atomic emission spectroscopy and inductively-coupled plasma spectrometry that required complicated sample preparation steps, and sophisticated instrumentation. In contrast to these highly expensive and time consuming methods, fluorogenic or chromogenic methods can be good alternatives for the detection of these species that provides high analyte sensitivity and selectivity, visual simplicity, instantaneous response, as well as real time monitoring. The rhodamine scaffold is an ideal template for the construction of metal ion chemosensors because they have large molar extinction coefficient, long excitation and emission wavelengths, high fluorescence quantum yields, good water solubility, and the potential for colorimetric and turn-on fluorescent detection. In this thesis work, we focus on design and synthesis of novel rhodamine based molecules for the detection of gold ions. Moreover, we investigate the photophysical properties of synthesized molecules in the absence and presence of gold ions in both synthetic samples and living cells.
  • Doctoral Thesis
    Design and Synthesis of Boron-Dipyrromethene (bodipy) Based Fluorescent and Colorimetric Sensors for the Detection of Gold and Mercury Ions
    (İzmir Institute of Technology, 2016) Üçüncü, Muhammed; Emrullahoğlu, Mustafa
    The identification and quantification of heavy metal ions such as gold and mercury species in synthetic samples and living cells have crucial importance for scientific research. Even at very low concentrations, heavy metal ions can cause mortal consequences. Hence, there is a huge need for the development of new, sensitive, and selective methods to detect biologically active molecules, heavy metal ions, and anions that have significant effects on humans or animals. Up to now, trace metal analyses have been performed by classical spectroscopic methods such as atomic absorption and emission spectroscopy and inductively coupled plasma mass spectrometry. However, these methodologies require sophisticated devices and must be preceded by complicated sample preparation steps. In contrast to these time-consuming and expensive methods, fluorogenic and chromogenic methods that have high analyte sensitivity and selectivity and easy sample preparation steps and that use cheaper instrumentation have become important alternatives in recent years. There are many organic dye molecules that act as signaling units for fluorogenic-sensing strategies such as rhodamine derivatives, fluorescein, coumarin, and BODIPY. For this thesis, we chose the BODIPY core as a signal reporter unit because of such unique properties as long excitation/emission wavelengths, high molar absorption coefficients and fluorescence quantum yields, and wide pH range for the sensing event. Also, BODIPY dyes can be easily derivatized from their various positions so that they produce an important advantage over other florophore molecules. The main purpose of this thesis is to design and synthesize new BODIPY derivatives that bear highly selective and sensitive receptor units towards gold and mercury ions. In addition, to investigate the photophysical properties of designed molecules in the absence and presence of targeted metal ions in both synthetic samples and living cells.