Sürdürülebilir Yeşil Kampüs Koleksiyonu / Sustainable Green Campus Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7755
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Article Citation - WoS: 10Citation - Scopus: 10Detection of Listeria Species in Fresh Fish and Fish Market Environment by Ims Technique in Turkey(Verlag M. und H. Schaper GmbH, 2011) Akkaya, Levent; Atabay, Halil İbrahim; Gök, Veli; Kara, RecepThe incidence of Listeria spp. was investigated in fresh fish (n = 100) sold at retail markets and in the environmental and personnel samples (n = 100) obtained from several fish markets in Afyonkarahisar, Turkey by immunomagnetic separation technique. The fish samples analysed included anchovy, trout, carp and grey mullet (25 of each). Six (6 %) of the fish samples were found positive for Listeria spp. and the overall incidence of Listeria spp. was 10 % in the environmental and personnel samples. Three Listeria spp., Listeria monocytogenes, L. ivanovii, L. grayi were recovered from the samples examined. In addition, L. seeligeri from a fish sample and L. innocua from an environmental sample (box) were isolated. It was found that L. monocytogenes was only detected in fresh water fish with an incidence of 8 %. For the environmental samples, knives and refrigerators had the highest (20 %) and personnel samples had the least (5%) levels of contamination. It can be concluded that the fish sold at retail markets are moderately contaminated with various species of Listeria including L. monocytogenes, which may pose a risk for human health. The presence of these bacteria in environmental and personnel samples of fish markets is also an important possible sources of cross-contamination of the fish and other seafood. The results of this study indicate the necessity of the implementation of good hygiene and sanitary practices in order to prevent and/or reduce the contamination of fishery products by Listeria spp. at the retail level.Article Citation - WoS: 13Citation - Scopus: 16Dilute-Acid Hydrolysis of Apple, Orange, Apricot and Peach Pomaces as Potential Candidates for Bioethanol Production(American Scientific Publishers, 2013) Üçüncü, Can; Tarı, Canan; Demir, Hande; Büyükkileci, Ali Oğuz; Özen, BanuChemical composition of four selected fruit pomaces (agro-industrial wastes) was evaluated. The effect of temperature, time, acid concentration and solid:liquid (S:L) ratio on dilute-acid hydrolysis of selected pomaces were investigated using 24 factorial and central composite design and optimum hydrolysis conditions were determined. A preliminary study was initiated using apple hydrolysate and the fungus Tricoderma harzianum in order to explore and demonstrate their potential uses in bioethanol production. Chemical composition of pomaces was promising as fermentation media. The highest reducing sugar yield from the optimization step was 31%, 49%, 56% and 52% for apple, apricot, orange and peach pomaces, respectively under optimum hydrolysis conditions. Neither furfural nor hydroxmethylfurfural (HMF) were detected in hydrolysates. The highest bioethanol production (1.67 g/L) was obtained in 6 days in a non-static incubator using Tricoderma harzianum pregrown in minimal medium. It was demonstrated that selected pomaces holds potential for bioethanol production and can be eliminated without being potential waste problem to the environment, with economical return. Although, this work is an initial study in showing the potential of these pomaces and T. harzianum in bioethanol production, an extensive study on the optimization of fermentation parameters is recommended for further increase of bioethanol production. Copyright © 2013 American Scientific Publishers All rights reserved.Article Citation - WoS: 2Citation - Scopus: 4Genotyping of Various Arcobacter Species Isolated From Domestic Geese by Randomly Amplified Polymorphic Dna (rapd) Analysis(M. & H. Schaper, 2008) Atabay, Halil İbrahim; Ünver, Ahmet; Otlu, Salih; Kalaycıoğlu, Atila TanerThe present study was undertaken to genotype Arcobacter (A.) butzleri, A. cryaerophilus and A. skirrowii isolates from domestic geese from three different flocks in Turkey. Fifteen Arcobacter isolates were analysed to determine the RAPD profiles based on the amplified DNA fragment patterns using a universal primer for genotyping. 7 A. cryaerophilus, 2 A. butzleri and 6 A. skirrowii isolates produced 6, 2 and 3 distinct profiles, respectively. The isolates of the same patterns originated from the same flocks. The findings of the present study may support previous reports of the existence of a large degree of heterogeneity among Arcobacter isolates. Observation of such levels of genetic diversity may suggest that there are multiple contamination sources in the environment and/or the determined genotypes may have undergone genetic rearrangements. This first report of genotyping of various Arcobacter species isolated from healthy geese is expected to improve the understanding of the ecology and epidemiology of this emerging pathogen.