Sürdürülebilir Yeşil Kampüs Koleksiyonu / Sustainable Green Campus Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7755
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Article Citation - WoS: 18Citation - Scopus: 20Assessment of Cd-Induced Genotoxic Damage in Urtica Pilulifera L. Using Rapd-Pcr Analysis(Taylor and Francis Ltd., 2016) Doğan, İlhan; Özyiğit, İbrahim İlker; Tombuloğlu, Güzin; Sakçalı, Mehmet Serdal; Tombuloğlu, HüseyinPlants can be used as biological indicators in assessing the damage done by bioaccumulation of heavy metals and their negative impact on the environment. In the present research, Roman nettle (Urtica pilulifera L.) was employed as a bioindicator for cadmium (Cd) pollution. The comparisons between unexposed and exposed plant samples revealed inhibition of the root growth (∼25.96% and ∼45.92% after treatment with 100 and 200 µmol/L Cd concentrations, respectively), reduction in the total soluble protein quantities (∼53.92% and ∼66.29% after treatment with 100 and 200 µmol/L Cd concentrations, respectively) and a gradual genomic instability when the Cd concentrations were increased. The results indicated that alterations in randomly amplified polymorphic DNA (RAPD) profiles, following the Cd treatments, included normal band losses and emergence of new bands, when compared to the controls. Also, the obtained data from F1 plants, utilized for analysis of genotoxicity, revealed that DNA alterations, occurring in parent plants due to Cd pollution, were transmitted to the next generation.Article Citation - WoS: 2Citation - Scopus: 4Genotyping of Various Arcobacter Species Isolated From Domestic Geese by Randomly Amplified Polymorphic Dna (rapd) Analysis(M. & H. Schaper, 2008) Atabay, Halil İbrahim; Ünver, Ahmet; Otlu, Salih; Kalaycıoğlu, Atila TanerThe present study was undertaken to genotype Arcobacter (A.) butzleri, A. cryaerophilus and A. skirrowii isolates from domestic geese from three different flocks in Turkey. Fifteen Arcobacter isolates were analysed to determine the RAPD profiles based on the amplified DNA fragment patterns using a universal primer for genotyping. 7 A. cryaerophilus, 2 A. butzleri and 6 A. skirrowii isolates produced 6, 2 and 3 distinct profiles, respectively. The isolates of the same patterns originated from the same flocks. The findings of the present study may support previous reports of the existence of a large degree of heterogeneity among Arcobacter isolates. Observation of such levels of genetic diversity may suggest that there are multiple contamination sources in the environment and/or the determined genotypes may have undergone genetic rearrangements. This first report of genotyping of various Arcobacter species isolated from healthy geese is expected to improve the understanding of the ecology and epidemiology of this emerging pathogen.