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Plant-On Devices for Sed Screening
(Izmir Institute of Technology, 2019) Yetgin, Ali; Pesen Okvur, Devrim; Canlı, Kerem
Thanks to their roots, plants take the required molecules from the soil, therefore their research is important. The fact that absorption takes place from the root hair makes the observations necessary. The inadequacy of traditional methods requires the development of new methods to ensure their observations, especially during root studies. The fact that chip systems have the features that can overcome the problems has caused plant researches by using this method. The fact that environmental factors can be imitated in chip systems paves the way for abiotic stresses to work. Although abiotic stresses have been studied extensively on plants, seed germination and subsequent root extension and root hair formation have not been studied in detail except for the model organism (Arabidopsis thaliana). The use of chip system for plant studies of scientists have begun the 21st century is also reveals the need to do more work in this area. Researchers show that they are excited about the advantages they bring to the system and that necessary studies will be carried out in other side branches. It is thought that more research will be done by using chip systems after a short time. By using chip systems, a suitable environment for seed growth can be provided and environmental conditions can be simulated. By creating the desired controlled environment, it is possible to create a system which results in less time and less cost in order to replace the inconvenient and expensive method.
Lab-on-a-chip devices for drug screening
(Izmir Institute of Technology, 2019) Gökçe, Begüm; Pesen Okvur, Devrim; Çağır, Ali
Breast cancer is one of the cancers with the highest incidence and mortality rates in women in Turkey as well as in the world. Tumor micro environment comprises of cancer and normal cells, extracellular matrix, soluble biological and chemical factors. Research has shown that cell shape, adhesion, migration, response to growth factors and drugs are different in 2D and 3D culture. Today, only 8 out of 100 anti-cancer clinical trial gives effective results. 3D cell culture systems have shown to be a necessary step between in vitro, in vivo and clinical studies. Therefore, it is necessary to better understand the interactions of cancer cells with their micro environment, for which new cell culture setups are required. The most apparent disadvantage of widely used 3D cell culture setups is the lack of stromal cells. The systems to be developed should both provide a 3D environment and comprise multiple cell types. The drug screen in 3D tri-culture method with a lab-on-a-chip device, that will be developed in this study will be able to answer these needs. Cell lines that represent different breast cancer types alone or together with stromal cells were cultured in 3D in the to be developed lab-on-a-chip; by determining the effects of drugs with different targets on the viability and distribution of cells, a drug screening method is developed.
Comparison Od Side Effects of Anti-Cancer Drugs in 2d and 3d And, Classical and Cell-On Cultures
(Izmir Institute of Technology, 2016) Kankale, Deniz; Pesen Okvur, Devrim; Çağır, Ali
The studies that aim to assess the effects of drugs developed against cancer at the cellular level use multiwell plates. However, these classical systems fail to reproduce the in-vivo like microenvironment necessary for realistic assessment. In addition, classical cell culture systems use high amount of materials increasing cost. On the other hand, lab-on-a-chip systems use minimal volumes of reagents and more importantly can mimic the in-vivo microenvironment via spatial and temporal control. Furthermore, it is known that cell response to drugs can be very different in 2D and 3D cell culture setups. Doxorubicin is a widely used anticancer drug. Here, doxorubicin uptake by highly metastatic human breast cancer cell line MDA-MB-231 and normal mammary epithelial cell line MCF10A were investigated using 2D and 3D, classical and cell-on-a-chip cultures. Drug uptake at 24, 48 and 72 hours various concentrations of the drug determined by measuring signal intensities from fluorescence microscopy images of cells. For cell viability assay, cells were stained with dapi and two cell lines were compared in systems. According to results, it was observed that 3D cell culture environment in chip provides more in-vivo like environment with less reagent consumption and cell viability is not correlated only with drug uptake.

Sürdürülebilir Yeşil Kampüs Koleksiyonu / Sustainable Green Campus Collection

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