Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik

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  • Article
    Evaluation of Aluminum Stress Induced Antibacterial and Antifungal Activities in Roman Nettle
    (Parlar Scientific Publications, 2019) Özyiğit, İbrahim İlker; Doğan, İlhan; Yalçın, İbrahim Ertuğrul; Severoğlu, Zeki
    In this current work, Roman nettle (Urtica pilulifera L.), a traditional medicinal plant that is very common and widespread species throughout Asia, Europe, and Northern Africa, was used as a model plant to investigate changes in antimicrobial activity following the application of aluminum stress. U pilulifera seedlings were grown in growth-room conditions and 0, 100, and 200 M AlCl3 were applied to the plants together with Hoagland solution (20 ml) for two months. The antimicrobial activities were tested against nine strains of bacteria (Salmonella sp., Staphyllococcus aureus, Escherichia coli, E. coli O157:H7 and Bacillus cereus) and fungus (Penicillum sp., Saccharomyces cerevisiae, Candida tropicans and C. albicans) by using the disc diffusion and agar well methods. The accumulated Al was measured by using ICP-OES in the leaves of studied plant samples. Additionally, a control group (water + 11.31 mg l(-1) Al) was prepared and applied to selected bacteria and fungi in order to understand the reason for obtained antimicrobial activities of Roman nettle is whether because of the compounds isolated from nettle leaves exposed to Al stress, or Al itself accumulated in leaves. The data proved that inhibitory antimicrobial effects were altered in U pilulifera upon the application of Al stress, especially on fungi species.
  • Article
    Citation - WoS: 3
    Ecophysiological Properties of Turkish Endemic Centaurea Consanguinea Dc.
    (Parlar Scientific Publications, 2019) Eskin, Bülent; Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, Göksel; Yarcı, Celal; Serin, Memduh
    This physiological and ecological study was performed on Centaurea consanguinea DC. to acquire information about certain characteristics (bioclimatic, edaphic, topographic, biotic etc.) of its habitat and distribution in Turkey, determine the requirements for germination, explore the soil-plant relations and obtain data on certain aspects of its population biology. Plant and co-located soil samples were collected from Amasya and Havza District (Samsun) in Turkey using standard methods. Various analyzes were employed for determining of physical, chemical and biological properties of the plant species and its co-located soils including pH, total protein and electrical conductivity (EC), soil texture, contents of mineral nutrition and other physical and chemical parameters and the results were presented. Nutrient amounts (in %) were found to be in the ranges of 0.01-0.007 for N, 0.0008-0.001 for P, 0.01-0.09 for K, 0.0002-0.0003 for Na in the soils and 2.25 for N, 0.0068 for P, 0.07 for K and 0.02 for Na in the plant, respectively. Also, it was observed that germination ratios of the seeds were depended on various factors.