Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik

Permanent URI for this collectionhttps://hdl.handle.net/11147/9

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Author: search.filters.author.Çelik, İbrahimInstitution Author: search.filters.institutionAuthor.Şelale, Hatice

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  • Article
    Citation - WoS: 24
    Citation - Scopus: 26
    Development of Est-Ssr Markers for Diversity and Breeding Studies in Opium Poppy
    (John Wiley and Sons Inc., 2013) Şelale, Hatice; Çelik, İbrahim; Gültekin, Visam; Allmer, Jens; Doğanlar, Sami; Frary, Anne
    All publicly available opium poppy expressed sequence tag (EST) sequences, totalling 20 885, were assembled into unigenes and examined for simple sequence repeats (SSRs). Nearly 19% of the 14 957 unigenes contained SSRs with 4% harbouring more than one SSR. Average density of the SSRs was 1 SSR per 3.6 kb of non-redundant EST sequence. Trinucleotide SSRs were most frequently identified (39%), and many of the most prevalent motifs were AT-rich. Flanking primers were designed for 86% of the SSRs and 67 primer pairs were tested on 37 opium poppy accessions and seven related species. All markers were transferable to the related species. Polymorphism information content (PIC) values for the markers were intermediate for comparisons within opium poppy (average of 0.27) and slightly higher for comparisons across species (average of 0.29). The markers were found to be useful for diversity analysis as they successfully distinguished among Turkish opium poppy accessions and land races.
  • Article
    Citation - WoS: 7
    Citation - Scopus: 6
    Water-Soluble Antioxidant Potential of Melon Lines Grown in Turkey
    (Taylor and Francis Ltd., 2012) Şelale, Hatice; Şığva, Hasan Özgür; Çelik, İbrahim; Doğanlar, Sami; Frary, Anne
    The antioxidant potential of 42 melon (Cucumis melo) lines including six cultivars grown in Turkey was assessed by measuring total water-soluble antioxidant capacity, phenolic and vitamin C contents. The lines showed significant variability for all three antioxidant parameters with breeding lines having higher antioxidant capacity and phenolic content than some popular cultivars. Different types of melons also showed significantly different antioxidant potentials. Thus, galia and ananas types showed a higher mean antioxidant capacity and phenolic content than the other tested types (yuva, kislik, canary, and charentais). Correlation analysis between antioxidant parameters showed a significant correlation between water-soluble antioxidant capacity and phenolic content. © 2012 Copyright Taylor and Francis Group, LLC.