Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Permanent URI for this collectionhttps://hdl.handle.net/11147/9
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Article Citation - WoS: 15Citation - Scopus: 16The Usability of Juniperus Virginiana L. as a Biomonitor of Heavy Metal Pollution in Bishkek City, Kyrgyzstan(Taylor & Francis, 2015) Kurmanbekova, Gülbübü; Severoğlu, Zeki; Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, Göksel; Yalçın, İbrahim Ertuğrul; Kaşoğlu, GültenUncontrolled and unplanned urbanization and industrialization due to increase of population and rapid industrial development have created severe environmental problems in Kyrgyzstan during the last few decades. In this study, Juniperus virginiana, a dioecious species, was employed in order to make assessment of the heavy metal pollution rate in the area and of the heavy metal pollution impact on the mineral nutrient status of the plant. For this study, leaf (washed and unwashed) and bark samples of J. virginiana, and its co-located soil samples were collected from eight different stations, all in the capital of Kyrgyzstan, Bishkek, in 2012 vegetation period. The standard procedures were used and the determinations of heavy metal and nutrient element contents (Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Na, Pb and Zn) in all samples were done using inductively coupled plasma-optical emission spectroscopy. According to our measurements, J. virginiana was found to be capable of accumulating a considerable amount of metals and the mineral nutrient uptake pattern was altered because of metal deposition in the plant, which showed a contamination risk in the area.Article Citation - WoS: 20Citation - Scopus: 19Using the Turkish Red Pine Tree To Monitor Heavy Metal Pollution(HARD, 2020) Yalçın, Ertuğrul; Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, Göksel; Yarcı, CelalTurkish red pine is an evergreen tree species widely distributed in the Aegean and Mediterranean regions in Turkey. In the present work, the heavy metal pollution level in Istanbul was investigated using Turkish red pine as a biomonitor. For determining heavy metal concentrations (Cd, Co, Cr, Mn, Ni and Pb) in leaf (unwashed and washed) and bark samples of Turkish red pine and its co-located soil samples we used ICP-OES. Additionally, particulate matter profiles of the research areas were investigated. The samples were collected from 5 different localities: 4 from the Bosporus region (Yildiz Grove, Fethi Pasha Grove, Baltalimani Grove and Mihrabad Grove) and one from Prince Island, also known as Buyukada (as control). According to our measurements, the highest heavy metal accumulations (in mg kg(-1) DW) in plant part samples were measured between 1.526 +/- 0.012 and 1.639 +/- 0.015 for Cd, 0.543 +/- 0.007 and 0.600 +/- 0.009 for Co, 5.110 +/- 0.064 and 5.648 +/- 0.072 for Cr, 9.347 +/- 0.105 and 10.331 +/- 0.111 for Mn, 7.483 +/- 0.091 and 8.271 +/- 0.096 for Ni, and 13.848 +/- 0.159 and 14.950 +/- 0.167 for Pb, while the highest heavy metal accumulations (in mg kg(-1) DW) in soil samples were measured between 1.813 +/- 0.021 and 1.974 +/- 0.029 for Cd, 6.326 +/- 0.082 and 6.992 +/- 0.091 for Co, 22.017 +/- 0.284 and 23.685 +/- 0.301 for Cr, 268.333 +/- 3.153 and 297.361 +/- 3.529 for Mn, 15.194 +/- 0.176 and 16.792 +/- 0.193 for Ni, and 68.778 +/- 0.715 and 74.514 +/- 0.883 for Pb. The highest outdoor particulate matter levels (in mu g/m(3)) in research areas were also determined as 27.103 for fine (PM2.5) and 67.792 for coarse (PM10) aerosols. The findings revealed that Turkish red pine could accumulate noteworthy amounts of heavy metals.Article Citation - WoS: 6Citation - Scopus: 9Detection of Physiological and Genotoxic Damages Reflecting Toxicity in Kalanchoe Clones(Global Network Environmental Science & Technology, 2016) Özyiğit, İbrahim İlker; Yılmaz, S.; Doğan, İlhan; Sakçalı, Mehmet Serdal; Tombuloğlu, Güzin; Demir, GökselIn order to make assessments in understanding of physiological and genotoxic effects of imposing cadmium (Cd) on photosynthetic pigment contents along with the changes occurring in genetic material of Kalanchoe plants were used in relation to various Cd-treatments. Young plantlets were originated from a single host plant as clones, and developed in vitro. Developed clones were grown in standard pots with daily watering of Hoagland solution (20 ml) containing different concentrations of cadmium chloride for two months. Cd concentrations of the collected samples were measured by employing ICP-OES and RAPD-PCR technique was applied for detecting the genotoxic effects of Cd. After two month of experimental period, the comparisons between unexposed and exposed Kalanchoe clone groups revealed reductions in photosynthetic pigment contents, especially at the highest level of Cd exposure and a genomic instability when application of Cd concentration increases. RAPD-PCR analyses demonstrated the distinguishable banding pattern in number and band intensities between Cd-treated and control groups. In addition, progressive Cd accumulations in leaves, stems and roots of plant samples were observed when the application of exposure level increased.Article Citation - WoS: 22Citation - Scopus: 25Influence of Aluminum on Mineral Nutrient Uptake and Accumulation in Urtica Pilulifera L.(Taylor and Francis Ltd., 2014) Doğan, İlhan; Özyiğit, İbrahim İlker; Demir, GökselPollutants can have detrimental effects on living organisms. They can cause toxicity, damaging cells, tissues and organs because of their high concentrations or activities. Plants provide a useful system for screening and monitoring environmental pollutants. Among pollutants, aluminum is considered as a primary growth limiting factor for plants resulting in decreased plant growth and development. Although considered to be a non-essential and highly toxic metal ion for growth and development, aluminum (Al) is easily absorbed by plants. Urticaceae family members have high nutrient requirements demonstrated by leaves containing high levels of calcium (Ca), iron (Fe), magnesium (Mg), and nitrogen (N). Urtica pilulifera is one of the important traditional medicinal plants in Turkey. In this study, U. pilulifera was used as a bioindicator to investigate the possible differences in the absorption and accumulation of mineral nutrients at different levels of the Al exposure and examine the mineral nutrition composition of U. pilulifera under Al stress. Also, some growth parameters (leaf-stem fresh and dry weights, root dry weights, stem lengths and leaf surface area) were investigated. U. pilulifera seedlings were grown for two months in growth-room conditions and watered with spiked Hoagland solution, which contained 0, 100, and 200 μM aluminium chloride (AlCl3). It was observed that macro- and micro-nutritional status of roots and leaves was altered by Al exposure. The concentrations of some macro- and micronutrients were reduced while concentrations of others were increased by excess of Al. Some macro- and micronutrients were increased at low level of Al whereas reductions were observed at high level of Al, and vice versa. The patterns were dependent on the macro- or micronutrient and the plant part.