Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Permanent URI for this collectionhttps://hdl.handle.net/11147/9
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Article Citation - WoS: 4Citation - Scopus: 4Development of Liposomal Formulations of the Eggplant Glycoalkaloids Solasonine and Solamargine(Elsevier, 2022) Tatlıdil, Engin; Gürbüz, Nergiz; Doğanlar, Sami; Frary, AnneThe eggplant glycoalkaloids solasonine and solamargine are efficient biomacromolecules against skin diseases but are water-insoluble which results in inefficient treatment due to inadequate transdermal delivery. To address this problem, several liposomal formulations were prepared and evaluated for parameters including lecithin type, hydration temperature, and pH. The optimal formula with high physical and chemical stability included the lecithin Phospholipon 80H hydrated with 10 mM NaCl (pH 5.5). Solasonine - solamargine loaded liposomes were tested for their physical and chemical stability and drug leakage over a three-month period. Furthermore, the drug release profile of the loaded liposomes was evaluated with different release media. The glycoalkaloids and their liposomal formulations were assessed for their biological activity in culture using HaCaT and SCC-25 cell lines. This work resulted in a biologically effective liposomal formulation that was stable (size <220 nm, PDI [removed]80%) for at least three months.Article Citation - WoS: 36Citation - Scopus: 47Mapping of Quantitative Trait Loci for Antioxidant Molecules in Tomato Fruit: Carotenoids, Vitamins C and E, Glutathione and Phenolic Acids(Elsevier, 2020) Gürbüz Çolak, Nergiz; Tek, Neslihan; Ülger, Mehmet; Frary, Anne; Doğanlar, SamiThe nutritional value of a crop lies not only in its protein, lipid, and sugar content but also involves compounds such as the antioxidants lycopene, beta-carotene and vitamin C. In the present study, wild tomato Solanum pimpinellifolium LA 1589 was assessed for its potential to improve antioxidant content. This wild species was found to be a good source of alleles for increasing beta-carotene, lycopene, vitamin C and vitamin E contents in cultivated tomato. Characterization of an LA 1589 interspecific inbred backcross line (IBL) mapping population revealed many individuals with transgressive segregation for the antioxidants confirming the usefulness of this wild species for breeding of these traits. Molecular markers were used to identify QTLs for the metabolites in the IBL population. In total, 64 QTLs were identified for the antioxidants and their locations were compared to the map positions of previously identified QTLs for confirmation. Four (57 %) of the carotenoid QTLs, four (36 %) of the vitamin QTLs, and 11 (25 %) of the phenolic acid QTLs were supported by previous studies. Furthermore, several potential candidate genes were identified for vitamins C and E and phenolic acids loci. These candidate genes might be used as markers in breeding programs to increase tomato's antioxidant content.Article Citation - WoS: 12Citation - Scopus: 14Transcriptomic Analysis of Selenium Accumulation in Puccinellia Distans (jacq.) Parl., a Boron Hyperaccumulator(Elsevier, 2020) Kök, Aysu Başak; Mungan, Mehmet Direnç; Doğanlar, Sami; Frary, AnneSelenium (Se) is present in a wide variety of natural and man-made materials on Earth. Plants are able to tolerate and (hyper)accumulate Se to different extents. In fact, some species can tolerate and accumulate multiple elements. Puccinellia distans (P. distans), weeping alkali grass, is known to hyperaccumulate extreme concentrations of boron and tolerate high levels of salinity, therefore, we investigated the Se accumulation and tolerance capacities of this species. In addition, P. distans' Se tolerance mechanism was studied using a transcriptomic approach. The results of this study indicated that, when grown in a hydroponic system containing 80 or 120 mu M Se, P. distans shoots accumulated from 1500 to 2500-fold more Se than plants grown without the element. Thus, P. distans was discovered to be a novel Se accumulator plant. RNA sequencing results and biochemical analyses helped to shed light on the Se tolerance and accumulation mechanism of P. distans. Here, we suggest that upregulation of Se assimilation and stress response genes may be due to induction of jasmonic acid signaling. In addition, we propose that the cell wall may play an important role in restriction of Se movement to the cytoplasm. Also, we hypothesize that Se accumulates in cells by sequestration of selenate in the vacuole. (C) 2019 Elsevier Ltd. All rights reserved.Article Citation - WoS: 18Citation - Scopus: 21Exploring Wild Alleles From Solanum Pimpinellifolium With the Potential To Improve Tomato Flavor Compounds(Elsevier, 2020) Gürbüz Çolak, Nergiz; Tek, Neslihan; Ülger, Mehmet; Frary, Anne; Doğanlar, SamiMost consumers complain about the flavor of current tomato cultivars and many pay a premium for alternatives such as heirloom varieties. Breeding for fruit flavor is difficult because it is a quantitatively inherited trait influenced by taste, aroma and environmental factors. A lack of genetic diversity in modern tomato cultivars also necessitates exploration of new sources for flavor alleles. Wild tomato S. pimpinellifolium and inbred backcross lines were assessed for individual sugars and organic acids which are two of the main components of tomato flavor. S. pimpinellifolium was found to harbor alleles that could be used to increase glucose and fructose content and adjust acidity by altering malic and citric acid levels. Single nucleotide polymorphism markers were used to detect 14 quantitative trait loci (QTLs) for sugars and 71 for organic acids. Confirmation was provided by comparing map locations with previously identified loci. Thus, seven (50 %) of the sugar QTLs and 22 (31 %) of the organic acids loci were supported by analyses in other tomato populations. Examination of the genomic sequence containing the QTLs allowed identification of potential candidate genes for several flavor components. © 2020 Elsevier B.V.Article Citation - WoS: 179Citation - Scopus: 229Health Benefits and Bioactive Compounds of Eggplant(Elsevier, 2018) Gürbüz, Nergiz; Uluışık, Selman; Frary, Anne; Frary, Amy; Doğanlar, SamiEggplant is a vegetable crop that is grown around the world and can provide significant nutritive benefits thanks to its abundance of vitamins, phenolics and antioxidants. In addition, eggplant has potential pharmaceutical uses that are just now becoming recognized. As compared to other crops in the Solanaceae, few studies have investigated eggplant's metabolic profile. Metabolomics and metabolic profiling are important platforms for assessing the chemical composition of plants and breeders are increasingly concerned about the nutritional and health benefits of crops. In this review, the historical background and classification of eggplant are shortly explained; then the beneficial phytochemicals, antioxidant activity and health effects of eggplant are discussed in detail.Article Citation - WoS: 18Citation - Scopus: 21Molecular Genetic Diversity in the Origanum Genus: Est-Ssr and Srap Marker Analyses of the 22 Species in Eight Sections That Naturally Occur in Turkey(Elsevier, 2018) Taşcıoğlu, Tülin; Sadıkoğlu, Narin; Doğanlar, Sami; Frary, AnneOriganum (L.) is a genus of herbaceous perennials with culinary and medicinal uses with many species found in the Mediterranean region. The taxonomic classification of species belonging to this genus has been studied using morphological, biochemical and genetic diversity analyses. In this study, the genetic diversity of 22 Turkish Origanum species (including 24 taxa from eight sections) was examined with 46 herbarium specimens from the Mediterranean, Eastern Anatolian, Central Anatolian, and Black Sea regions of Turkey. Molecular marker data were generated from 25 SRAP primer pairs and six EST-SSR primers which produced 325 alleles. Dendrogram, principal coordinate and population structure analyses revealed the relationships among Origanum sections, species and individuals. Gene flow (PhiPT and Nm) was also studied for a deeper understanding of the relationships and hybridization patterns between sections and species. Molecular separation of the Origanums roughly corresponded to the taxonomy that Ietswaart proposed in 1980 but also suggested that hybridization among sections and species may result in convergence and/or divergence of different sections and species.