Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik

Permanent URI for this collectionhttps://hdl.handle.net/11147/9

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Author: search.filters.author.Kartal Yandım, MelisInstitution Author: search.filters.institutionAuthor.Kiraz, Yağmur

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Now showing 1 - 3 of 3
  • Conference Object
    A Novel Biomarker for Drug Resistance in Chronic Myeloid Leukemia: Microrna-17
    (Elsevier Ltd., 2014) Baran, Yusuf; Kiraz, Yağmur; Fıratlıgil, Burcu; Baran, Yusuf; Kartal Yandım, Melis; Fıratlıgil Yıldırır, Burcu; Kiraz, Yağmur; Kozanoğlu, İlknur; Özdoğu, Hakan; Ünal, Ali; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    miRNAs are single stranded small RNA molecules (20–22 nt), which do not have ability to code for proteins. These types of RNAs play significant roles on gene regulation through inhibition of their target genes. In animals, most of miRNAs show their translational inhibitor effect on target mRNAs by semi-complementation to 3’UTR sequences of mRNAs and deadenylation that cause degradation of these mRNAs. The importance of miRNAs is increasing in cancer diagnosis and treatment since they are one of the major regulators of genes such as oncogenes, tumor suppressor genes. miR-17 is an oncogenic miRNA that suppress the activation of tumor suppressor genes like CDKN1A, p21 and E2F1. Based on previous information, we aimed to determine the correlation between expression levels of miR-17 microRNA in newly diagnosed, tyrosine kinase inhibitors treated and drug resistant CML patients.
  • Conference Object
    Effects of Placental Derived Mesenchymal Stem Cells on Experimental Asthma
    (Wiley, 2015) Micili, Cilaker S.; Kiraz, Yağmur; Sözmen, Çağlayan S.; Baran, Yusuf; Karaman, M.; Baran, Yusuf; Kartal Yandım, Melis; Kiraz, Yağmur; Karaman, O.; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    [No abstract available]
  • Article
    Citation - WoS: 12
    Citation - Scopus: 15
    Effects of Cell-Mediated Osteoprotegerin Gene Transfer and Mesenchymal Stem Cell Applications on Orthodontically Induced Root Resorption of Rat Teeth
    (Oxford University Press, 2017) Amuk, Nisa Gül; Kurt, Gökmen; Kiraz, Yağmur; Seyrantepe, Volkan; Kartal Yandım, Melis; Adan Gökbulut, Aysun; Akyıldız Demir, Seçil; Kiraz, Yağmur; Seyrantepe, Volkan; Baran, Yusuf; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    Aim: The aim of this study is to evaluate and compare therapeutic effects of mesenchymal stem cell (MSCs) and osteoprotegerin (OPG) gene transfer applications on inhibition and/or repair of orthodontically induced inflammatory root resorption (OIIRR). Materials and methods: Thirty Wistar rats were divided into four groups as untreated group (negative control), treated with orthodontic appliance group (positive control), MSCs injection group, and OPG transfected MSCs [gene therapy (GT) group]. About 100 g of orthodontic force was applied to upper first molar teeth of rats for 14 days. MSCs and transfected MSC injections were performed at 1st, 6th, and 11th days to the MSC and GT group rats. At the end of experiment, upper first molar teeth were prepared for genetical, scanning electron microscopy (SEM), fluorescent microscopy, and haematoxylin eosin-tartrate resistant acid phosphatase staining histological analyses. Number of total cells, number of osteoclastic cells, number of resorption lacunae, resorption area ratio, SEM resorption ratio, OPG, RANKL, Cox-2 gene expression levels at the periodontal ligament (PDL) were calculated. Paired t-test, Kruskal-Wallis, and chi-square tests were performed. Results: Transferred MSCs showed marked fluorescence in PDL. The results revealed that number of osteoclastic cells, resorption lacunae, resorption area ratio, RANKL, and Cox-2 were reduced after single MSC injections significantly (P < 0.05). GT group showed the lowest number of osteoclastic cells (P < 0.01), number of resorption lacunae, resorption area ratio, and highest OPG expression (P < 0.001). Conclusions: Taken together all these results, MSCs and GT showed marked inhibition and/or repair effects on OIIRR during orthodontic treatment on rats.