Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik

Permanent URI for this collectionhttps://hdl.handle.net/11147/9

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Author: search.filters.author.Taşcıoğlu, Tülin

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  • Article
    Molecular Genetic Characterization of the Turkish National Green Plum (prunus Cerasifera Ehrh.) Collection
    (Ege Tarımsal Araştırma Enstitüsü, 2021) Frary, Anne; Doğanlar, Sami; Çavdar, Andaç; Doğanlar, Sami; Frary, Amy; Frary, Anne; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    Plum is an important fruit worldwide and has high nutritional value. Prunus cerasifera Ehrh., a type of European plum species, is very popular in Turkey and is usually eaten at the green, unripe stage. In this work for the first time, the genetic diversity and population structure of the 66 accessions housed in the Turkish National P. cerasifera collection were investigated using molecular markers. A total of 47 Sequence-Related Amplified Polymorphism (SRAP) primer pairs were used and found to be highly polymorphic with 98% of the 495 amplified alleles providing polymorphism. Average diversity of the accessions was 0.39 as determined using the dice coefficient and was similar to P. cerasifera germplasm from France, Iran and Belarus but higher than that from China. This difference was expected as Turkey, Iran and Belarus are within the geographical origin of this species which was distributed to Europe during ancient times. The genetic relationships among accessions of the germplasm collection were assessed using unweighted neighbor joining dendrogram and population structure analyses. The dendrogram and population structure results were strongly correlated as both methods clustered the material into two main groups with a much smaller third admixed group. The analysis also indicated that Can and Papaz types, despite their morphological differences are not genetically distinct and provides information about genetic relationships that can be used in future plum breeding.
  • Article
    Citation - WoS: 18
    Citation - Scopus: 21
    Molecular Genetic Diversity in the Origanum Genus: Est-Ssr and Srap Marker Analyses of the 22 Species in Eight Sections That Naturally Occur in Turkey
    (Elsevier, 2018) Taşcıoğlu, Tülin; Sadıkoğlu, Narin; Doğanlar, Sami; Frary, Anne; Frary, Anne; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    Origanum (L.) is a genus of herbaceous perennials with culinary and medicinal uses with many species found in the Mediterranean region. The taxonomic classification of species belonging to this genus has been studied using morphological, biochemical and genetic diversity analyses. In this study, the genetic diversity of 22 Turkish Origanum species (including 24 taxa from eight sections) was examined with 46 herbarium specimens from the Mediterranean, Eastern Anatolian, Central Anatolian, and Black Sea regions of Turkey. Molecular marker data were generated from 25 SRAP primer pairs and six EST-SSR primers which produced 325 alleles. Dendrogram, principal coordinate and population structure analyses revealed the relationships among Origanum sections, species and individuals. Gene flow (PhiPT and Nm) was also studied for a deeper understanding of the relationships and hybridization patterns between sections and species. Molecular separation of the Origanums roughly corresponded to the taxonomy that Ietswaart proposed in 1980 but also suggested that hybridization among sections and species may result in convergence and/or divergence of different sections and species.