Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Permanent URI for this collectionhttps://hdl.handle.net/11147/9
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Article Citation - WoS: 15Citation - Scopus: 16The Usability of Juniperus Virginiana L. as a Biomonitor of Heavy Metal Pollution in Bishkek City, Kyrgyzstan(Taylor & Francis, 2015) Kurmanbekova, Gülbübü; Severoğlu, Zeki; Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, Göksel; Yalçın, İbrahim Ertuğrul; Kaşoğlu, GültenUncontrolled and unplanned urbanization and industrialization due to increase of population and rapid industrial development have created severe environmental problems in Kyrgyzstan during the last few decades. In this study, Juniperus virginiana, a dioecious species, was employed in order to make assessment of the heavy metal pollution rate in the area and of the heavy metal pollution impact on the mineral nutrient status of the plant. For this study, leaf (washed and unwashed) and bark samples of J. virginiana, and its co-located soil samples were collected from eight different stations, all in the capital of Kyrgyzstan, Bishkek, in 2012 vegetation period. The standard procedures were used and the determinations of heavy metal and nutrient element contents (Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Na, Pb and Zn) in all samples were done using inductively coupled plasma-optical emission spectroscopy. According to our measurements, J. virginiana was found to be capable of accumulating a considerable amount of metals and the mineral nutrient uptake pattern was altered because of metal deposition in the plant, which showed a contamination risk in the area.Article Citation - WoS: 6Citation - Scopus: 3Determining Element Accumulations in Turkish Red Pine Used as a Bioindicator for Estimating of Existing Pollution on Both Sides of Bosphorus in İstanbul(Parlar Scientific Publications, 2020) Yalçın, İbrahim Ertuğrul; Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, Göksel; Yarcı, CelalEvergreen Turkish red pine tree shows wide distribution around Aegean and Mediterranean regions in Turkey. Herein work investigated the mineral nutrient status of Turkish red pine as a bioindicator for revealing the impact of existing pollution on both sides of Bosphorus in Istanbul. For this, Al, B, Ca, Cu, Fe, K, Mg, Na and Zn concentrations were determined in unwashed and washed leaves and barks of the plant and soil samples. The standard procedures were applied and the determinations of element concentrations in all samples were done using ICP-OES. The sample collections were performed at five different locations in Istanbul, 4 from the Bosphorus region and one from Prince Island (as control). The highest element concentrations (in mg kg(-1) DW) in plant parts were recorded between 109.10 +/- 1.68 and 120.58 +/- 1.75 for Al, 10.18 +/- 0.14 and 12.52 +/- 0.17 for B, 8765.42 +/- 92.41 and 9600.69 +/- 102.22 for Ca, 10.91 +/- 0.13 and 11.73 +/- 0.16 for Cu, 226.85 +/- 3.01 and 254.07 +/- 3.20 for Fe, 4050.69 +/- 48.51 and 4477.08 +/- 52.34 for K, 794.58 +/- 9.82 and 878.33 +/- 10.07 for Mg, 1255.14 +/- 14.67 and 1374.31 +/- 18.55 for Na and 34.92 +/- 0.49 and 37.25 +/- 0.57 for Zn while the highest element concentrations (in mg kg(-1) DW) in co-located soil samples were measured between 5470.42 +/- 66.48 and 6046.25 +/- 73.54 for Al, 14.86 +/- 0.20 and 16.43 +/- 0.29 for B, 4600.56 +/- 55.22 and 4984.86 +/- 62.71 for Ca, 22.33 +/- 0.36 and 25.07 +/- 0.48 for Cu, 5500.01 +/- 71.05 and 5953.06 +/- 80.16 for Fe, 1819.44 +/- 23.51 and 2029.17 +/- 27.04 for K, 4108.75 +/- 50.77 and 4714.17 +/- 58.09 for Mg, 111.11 +/- 1.82 and 122.08 +/- 2.45 for Na and 117.10 +/- 2.33 and 126.86 +/- 2.61 for Zn.Article Evaluation of Aluminum Stress Induced Antibacterial and Antifungal Activities in Roman Nettle(Parlar Scientific Publications, 2019) Özyiğit, İbrahim İlker; Doğan, İlhan; Yalçın, İbrahim Ertuğrul; Severoğlu, ZekiIn this current work, Roman nettle (Urtica pilulifera L.), a traditional medicinal plant that is very common and widespread species throughout Asia, Europe, and Northern Africa, was used as a model plant to investigate changes in antimicrobial activity following the application of aluminum stress. U pilulifera seedlings were grown in growth-room conditions and 0, 100, and 200 M AlCl3 were applied to the plants together with Hoagland solution (20 ml) for two months. The antimicrobial activities were tested against nine strains of bacteria (Salmonella sp., Staphyllococcus aureus, Escherichia coli, E. coli O157:H7 and Bacillus cereus) and fungus (Penicillum sp., Saccharomyces cerevisiae, Candida tropicans and C. albicans) by using the disc diffusion and agar well methods. The accumulated Al was measured by using ICP-OES in the leaves of studied plant samples. Additionally, a control group (water + 11.31 mg l(-1) Al) was prepared and applied to selected bacteria and fungi in order to understand the reason for obtained antimicrobial activities of Roman nettle is whether because of the compounds isolated from nettle leaves exposed to Al stress, or Al itself accumulated in leaves. The data proved that inhibitory antimicrobial effects were altered in U pilulifera upon the application of Al stress, especially on fungi species.Article Citation - WoS: 6Citation - Scopus: 7Mineral Nutrient Acquisition by Cotton Cultivars Grown Under Salt Stress(Taylor and Francis Ltd., 2017) Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, Göksel; Yalçın, İbrahim ErtuğrulPhysiological responses were investigated in two cotton cultivars grown at various concentrations of sodium chloride (NaCl) in order to determine the degree of the tolerance of the cultivars to salt stress and understand the physiological responses with respect to utilization of mineral nutrients. After germination of the seeds of cotton cultivars, they were transferred into standard pots with 210 g sterilized compost and watered with 30 ml Hoagland’s solution containing different concentrations (0, 50, 100, 200, and 400 mM) of NaCl at two-day intervals for 3 months. Growth parameters were measured and the mineral nutrient analyses were done using inductively coupled plasma optical emission spectrometry (ICP-OES, Thermo Fisher Scientific, Waltman, MA). It was observed that plant growth and mineral nutritional status of both cultivars were altered extensively in those grown with NaCl. Excess NaCl reduces the concentrations of certain mineral nutrients and increases that of others, the patterns depending on the mineral nutrient and the plant part and varieties being compared to the control.Article Citation - WoS: 6Citation - Scopus: 8Mineral Element Uptake Status of Endemic Isoetes Anatolica Prada & Rolleri Populations From Bolu-Turkey(Pakistan Botanical Society, 2013) Özyiğit, İbrahim İlker; Doğan, İlhan; Eskin, Bülent; Keskin, Mustafa; Demir, Göksel; Yalçın, İbrahim ErtuğrulIsoetes genus is commonly known as the "quillworts" and considered to be "fern allies". There are about 200-250 species, with a cosmopolitan distribution but often scarce to rare. Isoetes genus members often grow in extremely sensitive aquatic environments such as temporary ponds, streams and lakes. They are therefore good indicators of environmental quality. Isoetes anatolica Prada & Rolleri is an endemic plant grows on calcareous sediment/soil on the edges of seasonal ponds located in a mountainous area near the southern coast of the Black Sea at 1400 m above sea level at Bolu, Turkey. In this study, mineral element uptake statuses of I. anatolica populations were studied on the background of plant-sediment/soil-water interactions. The study materials were collected from the place where this narrow endemic species only lives in the world (Abant Region, Bolu/Turkey) by using standard methods and plant and sediment/soil mineral element measurements (Al, B, Ca, Cu, Fe, K, Mg, Mn, Na, Ni and Zn) were done. ICP-OES was employed for the measurements during the study. Interrelations between mineral element contents in the sediment/soil, water and plant were discussed. The data revealed that I. anatolica is capable of accumulating considerable amounts of certain mineral elements (B, Ca, Mn and Na).Article Citation - WoS: 2Citation - Scopus: 2Distribution of Some Elements in Veronica Scutellata L. From Bolu, Turkey: Soil-Plant Interactions(Penerbit Universiti Kebangsaan Malaysia, 2013) Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, G.; Eskin, B.; Keskin, M.; Yalçın, İbrahim ErtuğrulVeronica scutellata L. occurs in moist and wet habitats, such as ponds, marshes and other wetlands. This study was conducted on this species to examine its mineral element uptake status in terms of interactions between soil and plant. Experimental materials were taken from the Southern coast of Black Sea at coordinates 40°36'N and 31°16'E at an altitude of 1400 m above sea level from Bolu - Turkey; using standard methods and plant (root, stem and leaf parts) and soil mineral element measurements (Al, B, Ca, Cu, Fe, K, Mg, Mn, Na, Ni and Zn) were done. During the study, ICP-OES was employed for the measurement of mineral elements. It was observed that considerable amounts of B, Ca, K, Mg, Mn, Na and Zn are accumulated by the plant.Article Citation - WoS: 18Citation - Scopus: 16Determination of Some Heavy Metals and Mineral Nutrients of Bay Tree (laurus Nobilis L.) in Bartin City, Turkey(Pakistan Botanical Society, 2012) Yaşar, Ülkühan; Özyiğit, İbrahim İlker; Yalçın, İbrahim Ertuğrul; Doğan, İlhan; Demir, GökselConcentrations of Al, Cd, Cu, Ni, and Pb in Laurus nobilis L. were examined for assessment of the impact of heavy metal exposure during winter periods, since these metals have the highest toxic potential. In this study, leaf (washed and unwashed), bark and branch samples of L. nobilis and soil samples were collected from 13 different localities, belonged to three stations. In conjunction with analyzing impact of the heavy metal exposure on the city using L. nobilis as a biomonitoring tool, the uptake and composition of mineral nutrients of L. nobilis were also investigated for determining the effects of heavy metals on mineral nutrition metabolism of the plant. The heavy metal and mineral nutrient concentrations of the collected samples were measured by using ICP-OES. The obtained data was analyzed with SPSS statistics program. As a result of measurements, the lowest and highest heavy metal accumulations and the amount of mineral nutrients measured in plants were as follows; Al (14.69-122.44 mg/kg d. wt), Cd (0.23-0.89 mg/kg d. wt), Cu (1.64-14.25 mg/kg d. wt.), Ni (0.001-0.45 mg/kg d. wt.), Pb (2.06-5.28 mg/kg d. wt.) and B (1.04- 6.67 mg/kg d. wt.), Ca (1195.34-4919.03 mg/kg d. wt.), Fe (17.13-203.25 mg/kg d. wt.), K (538.99-3778.37 mg/kg d. wt.), Mg(48.1-268.5 mg/kg d. wt.), Na (24.91-77.43 mg/kg d. wt.) and Zn (4.75-15.74 mg/kg d. wt.). According to the experimental data, the volume of the air pollution was analyzed and found significant in the city. Also, it was noticed that the metabolism of mineral nutrients of L. nobilis was altered by heavy metals. Finally, it was proved that L. nobilis is a suitable organism to be used as a biomonitoring tool for conducting research on heavy metal pollution.