Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Permanent URI for this collectionhttps://hdl.handle.net/11147/9
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Article Citation - WoS: 14Citation - Scopus: 16Antimicrobial Activity and Chemical Composition Screening of Epilobium Montanum Root(Association of Pharmaceutical Teachers of India, 2017) Canlı, Kerem; Yetgin, Ali; Akata, Ilgaz; Altuner, Ergin MuratMedical herbs have many bioactive component and they are used in microbial treatment since ancient time. The resistance of pathogen to antibiotic is became a critical problem, so novel antimicrobial agent related research is required. Epilobium montanum related antimicrobial research doesn’t exist, therefore root of this medicinal plant investigation was applied against 17 bacteria and 1 fungi by using disk diffusion method. These microbial species include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy. The results were presented that E. montanum has antimicrobial activity against all tested microbial species except E. coli. Seventeen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed by NMR spectra for 3D structure determination and identification.Article Citation - WoS: 15Citation - Scopus: 19Antimicrobial Activity and Chemical Composition Screening of Anacyclus Pyrethrum Root(Association of Pharmaceutical Teachers of India, 2017) Canlı, Kerem; Yetgin, Ali; Akata, Ilgaz; Altuner, Ergin MuratMedical herbs have many bioactive component and they are used in microbial treatment since ancient times. The resistance of pathogens to antibiotics became a critical problem, so researches for novel antimicrobial agents are required. Anacyclus pyrethrum (pellitory, Spanish chamomile or Mount Atlas daisy) is commonly used as a traditional medicine, therefore the antimicrobial activity of the root of this medicinal plant was investigated against 17 bacteria and 1 fungi by using disk diffusion method. The test strains include Bacillus, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella, Staphylococcus and Candida genera. Besides, chemical composition of this sample was determined by Gas Chromatography-Mass Spectroscopy analysis. The results were presented that A. pyrethrum has antimicrobial activity against all tested microbial species except E. faecalis and S. typhimurium. Eighteen major chemical components were determined, but some composition of this sample is not match with library. For this reason, this medicinal plant contain unknown molecules and this molecules should be analysed NMR spectra for 3D structure determination and identification.Article Citation - WoS: 21Citation - Scopus: 19In vitro antimicrobial screening of Aquilaria agallocha roots(African Ethnomedicines Network, 2016) Canlı, Kerem; Yetgin, Ali; Akata, Ilgaz; Altuner, Ergin Muratackground: It was previously shown that some parts of Aquilaria agallocha, which is commonly known as oud or oodh, such as roots have been used as a traditional medical herbal in different countries. In Turkey A. agallocha is one of the ingredients while preparing famous Mesir paste, which was invented as a medicinal paste and used from the Ottoman period to now at least for 500 years. The identification the in vitro antimicrobial activity of ethanol extract of A. agallocha roots is main purpose of this analysis. Materials and Methods: By using 17 bacteria and 1 fungi, which include Bacillus, Candida, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella and Staphylococcus genera, the activity of A. agallocha root extracts were analysed by the help of the disk diffusion method, that is one of the methods commonly used to determine antimicrobial activities. Results: As a result of the study it was observed that ethanol extracts of A. agallocha roots have a clear antimicrobial activity against nearly all microorganism used in the study, but only two bacteria namely E. coli ATCC 25922 and S. typhimurium SL 1344. Conclusion: According to the disk diffusion test results it may be possible to propose that A. agallocha roots should have a medicinal uses especially against E. faecium, L. monocytogenes ATCC 7644, B. subtilis DSMZ 1971, C. albicans DSMZ 1386, S. epidermidis DSMZ 20044 and S. aureus ATCC 25923.