Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik

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Institution Author: search.filters.institutionAuthor.Saygılı, TahsinSubject: search.filters.subject.Aggregatibacter actinomycetemcomitans

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  • Article
    Citation - WoS: 17
    Citation - Scopus: 16
    Aggregatibacter Actinomycetemcomitans Groel Protein Promotes Conversion of Human Cd4+ T Cells Into Ifn Gamma Il10 Producing Tbet+ Th1 Cells
    (Public Library of Science, 2012) Saygılı, Tahsin; Akıncılar, Semih Can; Akgül, Bünyamin; Nalbant Aldanmaz, Ayten
    One of the heat shock family protein (Hsp) expressing bacteria is the gram negative, periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa). A. actinomycetemcomitans' Hsp is a 64-kDa GroEL-protein, which has been shown to influence the host cells. In this study we used recombinant A. actinomycetemcomitans GroEL (rAaGroEL) protein as a model antigen to study GroEL-mediated T cell immune response. Human peripheral mononuclear cells (PBMCs), when stimulated with recombinant rAaGroEL, expressed early activation marker CD69 and IL-2R (CD25). CD25 and CD69 expressions were higher in CD4+ T cells compared to CD8+ T cells. rAaGroEL-responding CD4+ T cells expressed IL-10, IFNγ and TNFα cytokines. Interestingly, there were also IL-10 and IFNγ double cytokine producing CD4+ T cells. Additionally, IFNγ expressing CD4+ T cells were also T-bet positive. Altogether the results suggest that rAaGroEL protein affects CD4+ T cells to differentiate into IFNγ IL10-secreting T-bet+ Th1 cells.