Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik

Permanent URI for this collectionhttps://hdl.handle.net/11147/9

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  • Article
    Evaluation of Aluminum Stress Induced Antibacterial and Antifungal Activities in Roman Nettle
    (Parlar Scientific Publications, 2019) Özyiğit, İbrahim İlker; Doğan, İlhan; Yalçın, İbrahim Ertuğrul; Severoğlu, Zeki
    In this current work, Roman nettle (Urtica pilulifera L.), a traditional medicinal plant that is very common and widespread species throughout Asia, Europe, and Northern Africa, was used as a model plant to investigate changes in antimicrobial activity following the application of aluminum stress. U pilulifera seedlings were grown in growth-room conditions and 0, 100, and 200 M AlCl3 were applied to the plants together with Hoagland solution (20 ml) for two months. The antimicrobial activities were tested against nine strains of bacteria (Salmonella sp., Staphyllococcus aureus, Escherichia coli, E. coli O157:H7 and Bacillus cereus) and fungus (Penicillum sp., Saccharomyces cerevisiae, Candida tropicans and C. albicans) by using the disc diffusion and agar well methods. The accumulated Al was measured by using ICP-OES in the leaves of studied plant samples. Additionally, a control group (water + 11.31 mg l(-1) Al) was prepared and applied to selected bacteria and fungi in order to understand the reason for obtained antimicrobial activities of Roman nettle is whether because of the compounds isolated from nettle leaves exposed to Al stress, or Al itself accumulated in leaves. The data proved that inhibitory antimicrobial effects were altered in U pilulifera upon the application of Al stress, especially on fungi species.
  • Article
    Citation - WoS: 21
    Citation - Scopus: 19
    In vitro antimicrobial screening of Aquilaria agallocha roots
    (African Ethnomedicines Network, 2016) Canlı, Kerem; Yetgin, Ali; Akata, Ilgaz; Altuner, Ergin Murat
    ackground: It was previously shown that some parts of Aquilaria agallocha, which is commonly known as oud or oodh, such as roots have been used as a traditional medical herbal in different countries. In Turkey A. agallocha is one of the ingredients while preparing famous Mesir paste, which was invented as a medicinal paste and used from the Ottoman period to now at least for 500 years. The identification the in vitro antimicrobial activity of ethanol extract of A. agallocha roots is main purpose of this analysis. Materials and Methods: By using 17 bacteria and 1 fungi, which include Bacillus, Candida, Enterobacter, Enterococcus, Escherichia, Klebsiella, Listeria, Pseudomonas, Salmonella and Staphylococcus genera, the activity of A. agallocha root extracts were analysed by the help of the disk diffusion method, that is one of the methods commonly used to determine antimicrobial activities. Results: As a result of the study it was observed that ethanol extracts of A. agallocha roots have a clear antimicrobial activity against nearly all microorganism used in the study, but only two bacteria namely E. coli ATCC 25922 and S. typhimurium SL 1344. Conclusion: According to the disk diffusion test results it may be possible to propose that A. agallocha roots should have a medicinal uses especially against E. faecium, L. monocytogenes ATCC 7644, B. subtilis DSMZ 1971, C. albicans DSMZ 1386, S. epidermidis DSMZ 20044 and S. aureus ATCC 25923.