Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Permanent URI for this collectionhttps://hdl.handle.net/11147/9
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Article Citation - WoS: 6Citation - Scopus: 7Mineral Nutrient Acquisition by Cotton Cultivars Grown Under Salt Stress(Taylor and Francis Ltd., 2017) Özyiğit, İbrahim İlker; Doğan, İlhan; Demir, Göksel; Yalçın, İbrahim ErtuğrulPhysiological responses were investigated in two cotton cultivars grown at various concentrations of sodium chloride (NaCl) in order to determine the degree of the tolerance of the cultivars to salt stress and understand the physiological responses with respect to utilization of mineral nutrients. After germination of the seeds of cotton cultivars, they were transferred into standard pots with 210 g sterilized compost and watered with 30 ml Hoagland’s solution containing different concentrations (0, 50, 100, 200, and 400 mM) of NaCl at two-day intervals for 3 months. Growth parameters were measured and the mineral nutrient analyses were done using inductively coupled plasma optical emission spectrometry (ICP-OES, Thermo Fisher Scientific, Waltman, MA). It was observed that plant growth and mineral nutritional status of both cultivars were altered extensively in those grown with NaCl. Excess NaCl reduces the concentrations of certain mineral nutrients and increases that of others, the patterns depending on the mineral nutrient and the plant part and varieties being compared to the control.Article Citation - WoS: 11Citation - Scopus: 15Salinity Induced Changes in Cotton (gossypium Hirsutum L.)(Pakistan Botanical Society, 2012) Doğan, İlhan; Kekeç, Güzin; Özyiğit, İbrahim İlker; Sakçalı, Mehmet SerdalCotton (Gossypium hirsutum L.) is susceptible to abiotic stresses. High salinity is a common abiotic stress condition that adversely affects plant growth. Altered ion and water homeostasis changes due to NaCI stress, lead to molecular damage, growth arrest and even death. As a consequence of salt stress effects, secondary stresses such as oxidative damage by reactive oxygen species may occur. Reactive oxygen species can alter cellular metabolism through oxidative damage of lipids, proteins and nucleic acids causing lipid peroxidation, protein denaturing and DNA mutation. In recent years, several selective and sensitive assays have been developed to evaluate the effects of environmental stress on vegetal organisms. RAPD is one of them and developed for DNA analysis. In this study, cotton seedlings were used as bioindicator of salinity stress in the range of 50-400 mM. Effects of salinity stress were determined by comparing RAPD profiles of normal and treated cotton seedlings include variations in band intensities as well as gains or losses of band numbers. The DNA polymorphisms detected by RAPD analysis could be used as an investigation tool and useful biomarker assay for observing environmental stresses such as high salinity on vegetal organisms.Article Citation - WoS: 12Citation - Scopus: 15Mineral Element Distribution of Cotton (gossypium Hirsutum L.) Seedlings Under Different Salinity Levels(Pakistan Botanical Society, 2012) Doğan, İlhan; Özyiğit, İbrahim İlker; Demir, GökselCotton (Gossypium hirsutum L.) is the world's leading natural fiber and second largest oilseed crop. In addition to textile manufacturing, cotton and cotton-by products are the sources of wealth of consumer based products, livestock feed, fertilizer, foodstuff and paper. High concentrations of NaCl in soils account for large decreases in the yield of a wide variety of crops all over the world. The present study was conducted to evaluate NaCl stress on mineral nutrient composition of cotton due to its economic importance. Cotton seeds were germinated in Magenta vessels containing Murshige and Skoog (MS) media for 15 days and then transferred in sterile jars containing MS exposed to different levels of NaCl (50, 100, 200 and 400 mM) treatments for 1 month. Uptake of some mineral nutrients (B, Ca, Fe, K, Mg, Mn, Na and Zn) by the plants was examined in roots and leaves by using an Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES). The data proved that plant growth and uptake and accumulation of microelements are altered extensively in cotton grown with NaCl. Excess NaCl reduces the uptake pattern of certain elements and increases that of others, the patterns depending on the element and the plant part being compared to the control.