Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Permanent URI for this collectionhttps://hdl.handle.net/11147/9
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Article Citation - WoS: 15Citation - Scopus: 15Physical Mapping of Nbs-Coding Resistance Genes To the Me-Gene Cluster on Chromosome P9 Reveals Markers Tightly Linked To the N Gene for Root-Knot Nematode Resistance in Pepper(Springer Verlag, 2016) Çelik, İbrahim; Söğüt, Mehmet Ali; Özkaynak, Ercan; Doğanlar, Sami; Frary, AnneNatural root-knot nematode resistance genes are unique resources to control this major pest in pepper (Capsicum annuum). Although four genes (Me1, Me3, Me7 and N) conferring broad-spectrum resistance were mapped to a cluster in a 28-cm interval on chromosome P9, limited markers targeting this region were available. In the present study, the Me-gene cluster was structurally annotated for resistance genes to develop markers targeting the N gene. As a result, the Me-gene cluster (4.07 Mb in size) was found to contain three resistance gene hotspots. In addition, a SSR maker tightly linked to the N gene (0.8 cM away) was developed for marker-assisted selection in pepper.Article Citation - WoS: 13Citation - Scopus: 22Development of a Snp-Based Caps Assay for the Me1 Gene Conferring Resistance To Root Knot Nematode in Pepper(Springer Verlag, 2015) Uncu, Ali Tevfik; Çelik, İbrahim; Devran, Zübeyir; Frary, Anne; Frary, Amy; Doğanlar, SamiRoot knot nematodes (Meloidogyne spp.) are significant agricultural pests on many crops, including pepper (Capsicum annuum). Host plant resistance offers the most sustainable means of controlling this pest. A cluster of genes on chromosome 9 confers resistance, with Me1 providing protection against three nematode species: M. incognita, M. javanica and M. arenaria. We describe the development of a codominant CAPS marker located 1.13 cM away from the Me1 gene. This marker should be useful for marker assisted selection of nematode resistance in pepper breeding programs.
