Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik

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  • Article
    Citation - WoS: 15
    Citation - Scopus: 15
    Physical Mapping of Nbs-Coding Resistance Genes To the Me-Gene Cluster on Chromosome P9 Reveals Markers Tightly Linked To the N Gene for Root-Knot Nematode Resistance in Pepper
    (Springer Verlag, 2016) Çelik, İbrahim; Söğüt, Mehmet Ali; Özkaynak, Ercan; Doğanlar, Sami; Frary, Anne
    Natural root-knot nematode resistance genes are unique resources to control this major pest in pepper (Capsicum annuum). Although four genes (Me1, Me3, Me7 and N) conferring broad-spectrum resistance were mapped to a cluster in a 28-cm interval on chromosome P9, limited markers targeting this region were available. In the present study, the Me-gene cluster was structurally annotated for resistance genes to develop markers targeting the N gene. As a result, the Me-gene cluster (4.07 Mb in size) was found to contain three resistance gene hotspots. In addition, a SSR maker tightly linked to the N gene (0.8 cM away) was developed for marker-assisted selection in pepper.