Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik

Permanent URI for this collectionhttps://hdl.handle.net/11147/9

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Subject: search.filters.subject.Salt stress

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  • Article
    Citation - WoS: 2
    Citation - Scopus: 2
    Characterization of the Beta1 Gene, Which Might Play a Role in Beta Vulgaris Subsp. Maritima Salt Tolerance
    (Türkiye Klinikleri Journal of Medical Sciences, 2017) Uysal, Özge; Çakıroğlu, Çiğdem; Karakaya, Hüseyin Çağlar; Karakaya, Hüseyin Çağlar; Koç, Ahmet; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    Salinity stress has a negative impact on plant growth, which affects homeostasis and productivity. The uptake of nonessential salt ions changes the osmotic balance of the cell and causes dehydration. Higher plants develop salt tolerance mechanisms to avoid dehydration. Sea beet (Beta vulgaris subsp. maritima) is a halophytic ancestor of cultivated sugar beet that displays salt stress tolerance. In this study, we screened a B. vulgaris subsp. maritima cDNA library in Saccharomyces cerevisiae strain Ab11c (ena1Δ, nha1/4Δ, nhx1Δ), which is deficient in sodium transport, to find sodium-detoxifying genes. We identified a cDNA construct, named BETA1, providing salt tolerance to yeast cells. This gene had no previously described function. Intracellular sodium measurements demonstrated no significant differences between yeast cells expressing BETA1 or a sham vector, suggesting that sodium was not effluxed in BETA1-expressing cells. Transcriptionally, BETA1 mRNA levels were induced immediately in leaves and later in the root system in response to the salt stress. Our results suggest that the BETA1 gene is part of the salt tolerance network in B. vulgaris subsp. maritima.
  • Article
    Citation - WoS: 12
    Citation - Scopus: 15
    Mineral Element Distribution of Cotton (gossypium Hirsutum L.) Seedlings Under Different Salinity Levels
    (Pakistan Botanical Society, 2012) Doğan, İlhan; Doğan, İlhan; Özyiğit, İbrahim İlker; Demir, Göksel; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of Science; 01. Izmir Institute of Technology
    Cotton (Gossypium hirsutum L.) is the world's leading natural fiber and second largest oilseed crop. In addition to textile manufacturing, cotton and cotton-by products are the sources of wealth of consumer based products, livestock feed, fertilizer, foodstuff and paper. High concentrations of NaCl in soils account for large decreases in the yield of a wide variety of crops all over the world. The present study was conducted to evaluate NaCl stress on mineral nutrient composition of cotton due to its economic importance. Cotton seeds were germinated in Magenta vessels containing Murshige and Skoog (MS) media for 15 days and then transferred in sterile jars containing MS exposed to different levels of NaCl (50, 100, 200 and 400 mM) treatments for 1 month. Uptake of some mineral nutrients (B, Ca, Fe, K, Mg, Mn, Na and Zn) by the plants was examined in roots and leaves by using an Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES). The data proved that plant growth and uptake and accumulation of microelements are altered extensively in cotton grown with NaCl. Excess NaCl reduces the uptake pattern of certain elements and increases that of others, the patterns depending on the element and the plant part being compared to the control.