Bioengineering / Biyomühendislik
Permanent URI for this collectionhttps://hdl.handle.net/11147/4529
Browse
2 results
Search Results
Article Citation - WoS: 11Citation - Scopus: 11Absorbance-Based Detection of Arsenic in a Microfluidic System With Push-And Pumping(Elsevier, 2021) Karakuzu, Betül; Gülmez, Yekta; Tekin, H. CumhurRapid and portable analysis of arsenic (As) contamination in drinking water is very important due to its adverse health effects on humans. Available commercial detection kits have shown low sensitivity and selectivity in analysis, and also they can generate harmful by-products. Microfluidic-based approaches allow portable analysis with gold nanoparticles (AuNPs) as labels. However, they need complex surface modification steps that complicate detection protocols. Due to the lack of precise sensing and affordable solution, we focused on developing a microfluidic platform that uses a push-and-pull pumping method for sensitive detection of As. In this detection principle, a sample is introduced in the microfluidic channel modified with -SH functional groups where As can bind. Then, AuNPs are given in the channel and AuNPs bind on free -SH functional groups which are not allocated with As. Absorbance measurements are conducted to detect AuNPs absorbed on the surfaces and the resulting absorbance value is inversely proportional with As concentration. The method enables detection of As down to 2.2 mu g/L concentration levels in drinking water, which is well-below the allowed maximum As concentration of 10 mu g/L in the drinking waters by the World Health Organization (WHO). The paper reveals that multiple push-and-pull pumping of fixed volume of sample and AuNPs with a syringe pump can improve the binding efficiency in the microfluidic channel. With this technique, low amounts of sample (1 mL) and short total assay time (25 min) are sufficient to detect As.Conference Object Electromechanical Lab-On Platform for Creatinine Analysis Using Automated Elisa Protocols(Chemical and Biological Microsystems Society, 2020) Karakuzu, Betül; Tarım, Ergün Alperay; Öksüz, Cemre; Tekin, Hüseyin CumhurWe present an electromechanical lab-on-a-chip (LOC) platform for the automated serum creatinine detection applying enzyme-linked immunosorbent assay (ELISA) principle. In the platform, antibody covered bar selectively captures the creatinine in the sample and the electromechanical system allows automatic movement between the designed reservoirs containing assay solutions. At the end of the protocol, the absorbance value of the appeared color is measured to determine creatinine concentration in the sample. Since this system allows measuring automatically creatinine levels with minimum time and cost, it can be utilized for point-of-care monitoring of chronic kidney diseases (CKD) for the future. © 2020 CBMS-0001