Bioengineering / Biyomühendislik

Permanent URI for this collectionhttps://hdl.handle.net/11147/4529

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Access Right: Open AccessAuthor: search.filters.author.Tüncel, Özge

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  • Article
    Citation - WoS: 13
    Citation - Scopus: 13
    Single Chain Cationic Polymer Dot as a Fluorescent Probe for Cell Imaging and Selective Determination of Hepatocellular Carcinoma Cells
    (American Chemical Society, 2019) Özenler, Sezer; Yücel, Müge; Tüncel, Özge; Kaya, Hakan; Özçelik, Serdar; Yıldız, Ümit Hakan
    This letter describes formation of single chain cationic polymer dots (Pdots) made of poly[1,4-dimethy1-1-(34(2,4,5-trimethylthiophen-3-yl)oxy)propyl)piperazin-1-ium bromide] conjugated polyelectrolyte (CPE). The single chain Pdot formation relies on a simple process which is a rapid nanophase separation between CPE solution of ethylene glycol and water. Pdots show narrow monodisperse size distribution with a 3.6 nm in diameter exhibiting high brightness and excellent colloidal and optical stability. It has been demonstrated that photoluminescent Pdots provide selective nuclear translocation to hepatocellular carcinoma cells as compared to healthy liver cells. The Pdot labeling effectively discriminates cancer cells in the coculture media. Pdots hold great promise as a luminescent probe to diagnose cancer cells in histology and may guide surgeons during operations to precisely separate out cancerous tissue due to augmented fluorescence brightness.
  • Article
    Citation - WoS: 8
    Wound Healing Effects of Various Fractions of Olive Leaf Extract (ole) on Mouse Fibroblasts
    (Ars Docendi, 2018) Erdoğan, İpek; Bayraktar, Oğuz; Uslu, Mehmet Emin; Tüncel, Özge
    Olive (Olea europaea) leaf has been introduced as a potential therapeutic in wound healing owing to combined antioxidant and antimicrobial activity. Comparison of crude extract and its fractions in terms of antioxidant capacity, antimicrobial and cytotoxic activity to gain insight about cell migration rate under exogenous stress of H2O2, as a hallmark of wound healing constituted the objective of this study. Oleuropein-containing fraction exerted the highest cell migration rate among other fractions that contains hydroxytyrosol, verbascoside and luteolin, whilst treatment with high concentrations (50 mu g/ml) of this fraction simultaneously with H2O2 caused a dramatic decline in cell migration, resulting in the loss of cell adherence. Results overall indicated that active compounds caused an imbalance in redox signaling beyond a critical concentration. Comparison of fractions and crude extract also revealed that crude extract promoted cell migration by 20%, which may be attributed to synergistic effect of undefined phenolics.