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Citation - WoS: 6
Citation - Scopus: 5
Basidiomycota Species in Drosophila Gut Are Associated With Host Fat Metabolism
(Nature Research, 2023) Bozkurt, Berkay; Terlemez, Gamze; Sezgin, Efe
The importance of bacterial microbiota on host metabolism and obesity risk is well documented. However, the role of fungal microbiota on host storage metabolite pools is largely unexplored. We aimed to investigate the role of microbiota on D. melanogaster fat metabolism, and examine interrelatedness between fungal and bacterial microbiota, and major metabolic pools. Fungal and bacterial microbiota profiles, fat, glycogen, and trehalose metabolic pools are measured in a context of genetic variation represented by whole genome sequenced inbred Drosophila Genetic Reference Panel (DGRP) samples. Increasing Basidiomycota, Acetobacter persici, Acetobacter pomorum, and Lactobacillus brevis levels correlated with decreasing triglyceride levels. Host genes and biological pathways, identified via genome-wide scans, associated with Basidiomycota and triglyceride levels were different suggesting the effect of Basidiomycota on fat metabolism is independent of host biological pathways that control fungal microbiota or host fat metabolism. Although triglyceride, glycogen and trehalose levels were highly correlated, microorganisms’ effect on triglyceride pool were independent of glycogen and trehalose levels. Multivariate analyses suggested positive interactions between Basidiomycota, A. persici, and L. brevis that collectively correlated negatively with fat and glycogen pools. In conclusion, fungal microbiota can be a major player in host fat metabolism. Interactions between fungal and bacterial microbiota may exert substantial control over host storage metabolite pools and influence obesity risk. © 2023, Springer Nature Limited.
Expression of Steroidogenic Enzymes in Placentome of Ewes With Pregnancy Toxemia After Two Parturition Induction Methods
(Hellenic Veterinary Medical Society, 2023) Risvanlı, A.; Özalp, G. R.; Ortaç, C. T.; Bozkurt, Berkay; Aktar, A.; Yavuz, A.; Korlu, Y.; Şeker, İ.
The regulation pattern of important enzymes in placental steroidogenesis and prostaglandin production in ewes with pregnancy toxemia is reviewed. The alterations of gene expressions after the administration of aglepristone (AG) and dexamethasone (DEX) are also discussed. Four healthy (CG) and 22 ewes with experimental pregnancy toxemia were included in the study. Ewes with pregnancy toxemia of group AG (n=9) and group DEX (n=9) were injected twice with 10 mg/kg of aglepristone and once with 5 ml dexamethasone respectively to induce parturition on 141 & PLUSMN;1,3 day of gestation; whereas healthy control [Group CG (n=4)] and pregnancy toxemia [Group PT (n=4)] group received no treatment for parturition induction. Placentomes were immediately collected right after the expulsion of the last lamb. mRNA extraction from total placentome capsule, cotyledon and caruncle was carried out and Real-Time PCR was performed. Serum samples were collected from ewes and cortisol, PGFM, PGE2, estrone sulfate and progesterone concentrations were measured after treatments until parturition. The lowest mRNA expressions of steroidogenic enzymes were detected in group PT. Interestingly expression pattern of steroidogenic enzymes in group AG was similar to group PT. No difference was found in mRNA expressions of 3 & beta;HSD and CYP19 among groups. Between groups, AG-DEX the mRNA expressions in the caruncle of PTGS2/COX2 and PGFS were statistically different respectively (P<0.005). A significant difference could be observed in EP3 expression in the caruncle of DEX and AG compared to CG (P<0.05); however PTGES, EP1, EP2, and EP4 expressions were not statistically different among groups (P>0,05). Estrone sulfate, PGE,2 and PGFM concentrations were statistically different, however, no difference was observed in cortisol levels between groups. The present study suggests that the endocrinologic pathway controlling parturition is different in ewes with pregnancy toxemia. Dexamethasone administration endocrinologically mimicked normal partu-rition, but the genes regulating uterine contractions were similarly expressed, as in group PT. Probably expressions of EP1 and tissue-specific counter-expressions of cervical EP genes could refer to the pathogenesis of insufficient cervical dilatation, observed in pregnancy toxemia and dexamethasone applications.
Citation - WoS: 1
Citation - Scopus: 1
Expression Profile of Prostaglandin Enzymes in Cystic Endometrial Hyperplasia in Dogs: the Results of a Hypothesis in Clinical Trial
(Kafkas Üniversitesi, 2023) Korlu, Yeşim; Yavaş, Özkan; Aktar, Ahmet; Bozkurt, Berkay; Özyiğit, Musa Özgür; Özalp, Gözde Rabia
The expressions of prostaglandin synthesis enzymes and estrogen, progesterone receptors in canine cystic endometrial hyperplasia (CEH) were reported in this manuscript. Uterine tissue samples were collected from bitches with CEH (n=5), CEH-P (Cystic endometrial hyperplasia-Pyometra) (n=5) and healthy-negative control group, CG (n=5). Immunohistochemistry was carried out for the estrogen (ER) and progesterone receptor (PR) detection. Shock-frozen samples were utilized in mRNA extraction and Real-Time PCR was performed. Gene expression of PTGS2/COX2, PTGES, PTGER4, PGFS, PTGFR and PGR were detected higher in the CEH group compared with CG. The PGFS and PTGFR (FP) mRNA expressions were significantly increased in CEH compared with other groups. Expression of progesterone receptor mRNA (PGR) was highest in CEH and statistically different from the CEH-P group (P<0.05). No PR immunostaining was observed. ER staining had been detected in endometrial glands, endometrial stoma and myometrium, however hyperplasic glands in propria mucosa had lower or no ER scores. Based on the results of this study, the high levels of prostaglandin enzymes and low ER scores in CEH could be a preliminary step for the next stages of severe differentiation of endometrium.

Bioengineering / Biyomühendislik

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