Bioengineering / Biyomühendislik
Permanent URI for this collectionhttps://hdl.handle.net/11147/4529
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Correction Citation - WoS: 2Citation - Scopus: 2Correction: Scaffold-Free Three-Dimensional Cell Culturing Using Magnetic Levitation(Royal Society of Chemistry, 2018) Türker, Esra; Demircak, Nida; Arslan Yıldız, AhuThe authors regret the inclusion of an incorrect figure caption for Fig. 2. The corrected figure caption for Fig. 2 is shown below. Fig. 2 Evaluation of levitation height (z) and density profiles through magnetic levitation. (A) Gd(III) chelates were named as Gx (Gadovist/Gadobutrol), Dx (Dotarem/Gadoteric acid) and Ox (Omniscan/Gadodiamide). (B) Standard curve for PE bead density against levitation height; linear curve fitting gives the standard function for the corresponding curve. (C–E) Levitation height profiles of single NIH 3T3 cells under 30/50/100/200 mM Gd concentrations. Single cell density profiles calculated through standard function of linear fitting.Article Citation - WoS: 34Citation - Scopus: 36Biomimetic Hybrid Scaffold Consisting of Co-Electrospun Collagen and Pllcl for 3d Cell Culture(Elsevier Ltd., 2019) Türker, Esra; Yıldız, Ümit Hakan; Arslan Yıldız, AhuElectrospun collagen is commonly used as a scaffold in tissue engineering applications since it mimics the content and morphology of native extracellular matrix (ECM) well. This report describes "toxic solvent free" fabrication of electrospun hybrid scaffold consisting of Collagen (Col) and Poly(L-lactide-co-epsilon-caprolactone) (PLLCL) for three-dimensional (3D) cell culture. Biomimetic hybrid scaffold was fabricated via co-spinning approach where simultaneous electrospinning of PLLCL and Collagen was mediated by polymer sacrificing agent Polyvinylpyrrolidone (PVP). Acidified aqueous solution of PVP was used to solubilize collagen without using toxic solvents for electrospinning, and then PVP was readily removed by rinsing in water. Mechanical characterizations, protein adsorption, as well as biodegradation analysis have been conducted to investigate feasibility of biomimetic hybrid scaffold for 3D cell culture applications. Electrospun biomimetic hybrid scaffold, which has 3D-network structure with 300-450 nm fiber diameters, was found to be maximizing cell adhesion through assisting NIH 3T3 mouse fibroblast cells. 3D cell culture studies confirmed that presence of collagen in biomimetic hybrid scaffold have created a major impact on cell proliferation compared to conventional 2D systems on long-term, also cell viability increased with the increasing amount of collagen. (c) 2019 Elsevier B.V. All rights reserved.Article Citation - WoS: 75Citation - Scopus: 74Scaffold-Free Three-Dimensional Cell Culturing Using Magnetic Levitation(Royal Society of Chemistry, 2018) Türker, Esra; Demirçak, Nida; Arslan Yıldız, AhuThree-dimensional (3D) cell culture has emerged as a pioneering methodology and is increasingly utilized for tissue engineering, 3D bioprinting, cancer model studies and drug development studies. The 3D cell culture methodology provides artificial and functional cellular constructs serving as a modular playground prior to animal model studies, which saves substantial efforts, time and experimental costs. The major drawback of current 3D cell culture methods is their dependency on biocompatible scaffolds, which often require tedious syntheses and fabrication steps. Herein, we report an easy-to-use methodology for the formation of scaffold-free 3D cell culture and cellular assembly via magnetic levitation in the presence of paramagnetic agents. To paramagnetize the cell culture environment, three different Gadolinium(iii) chelates were utilized, which led to levitation and assembly of cells at a certain levitation height. The assembly and close interaction of cells at the levitation height where the magnetic force was equilibrated with gravitational force triggered the formation of complex 3D cellular structures. It was shown that Gd(iii) chelates provided an optimal levitation that induced intercellular interactions in scaffold-free format without compromising cell viability. NIH 3T3 mouse fibroblasts and HCC827 non-small-cell lung cancer cells were evaluated via the magnetic levitation system, and the formation of 3D cell culture models was validated for both cell lines. Hereby, the developed magnetic levitation system holds promises for complex cellular assemblies and 3D cell culture studies.Article Citation - WoS: 46Citation - Scopus: 57Recent Advances in Magnetic Levitation: a Biological Approach From Diagnostics To Tissue Engineering(American Chemical Society, 2018) Türker, Esra; Arslan Yıldız, AhuThe magnetic levitation technique has been utilized to orientate and manipulate objects both in two dimensions (2D) and three dimensions (3D) to form complex structures by combining various types of materials. Magnetic manipulation holds great promise for several applications such as self-assembly of soft substances and biological building blocks, manipulated tissue engineering, as well as cell or biological molecule sorting for diagnostic purposes. Recent studies are proving the potential of magnetic levitation as an emerging tool in biotechnology. This review outlines the advances of newly developing magnetic levitation technology on biological applications in aqueous environment from the biotechnology perspective.Conference Object Üç Boyutlu Hücre Kültürü için Polimer Esaslı Ekstrasellüler Matriks Mimetiği(Institute of Electrical and Electronics Engineers Inc., 2017) Türker, Esra; Yıldız, Ümit Hakan; Arslan Yıldız, AhuElektro-eğirme metodu gelişmiş üretim teknolojilerindendir ve biyomedikal uygulamalarında yaygın olarak kullanılmaktadır. Özellikle doku mühendisliğinde amaç, çalışılacak doku üzerine doğal veya sentetik destek materyali (iskele) üreterek hücrenin uyum sağlayabileceği bir ortam oluşturmaktır. Bu projenin amacı üç boyutlu (3D) hücre kültürü çalışmaları için elektro-eğirme-metodu ile poli(L-laktik-co-epsilon-kaprolakton) (PLLCL) kullanılarak iskele üretilmesidir. Homojen lifler ve uygun gözenek boyutu elde etmek amacıyla optimizasyon çalışmaları yapılmıştır. Elde edilen liflerin çapı, akış hızı ve voltajın artmasıyla azalmaktadır. Taramalı uç elektron mikroskop incelemeleri (SEM) lif morfolojik yapılarının doku iskelesi fabrikasyonu için ideale yakın olduğunu ortaya çıkarmıştır.