Bioengineering / Biyomühendislik

Permanent URI for this collectionhttps://hdl.handle.net/11147/4529

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COAR Access Rights: search.filters.coarAccessRights.metadata only accessPublisher: search.filters.publisher.Mary Ann Liebert

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  • Conference Object
    Biopatterning of 3d Cellular Structures Via Contactless Magnetic Manipulation for Drug Screening
    (Mary Ann Liebert, 2023) Onbas, Rabia; Arslan Yıldız, Ahu
  • Conference Object
    Biofabrication of Scaffold-Free 3d Cellular Structures Using Magnetic Levitational Assembly To Study Cardiac Toxicity
    (Mary Ann Liebert, 2023) Yıldız, Ahu Arslan; Arslan Yıldız, Ahu; Onbaş, Rabia
    Spheroids are one of the well-characterized 3D cell culture approaches for drug screening and therapeutic studies. Magnetic levitation (MagLev) is a newly developing approach to form 3D cellular structures and spheroids [1,2,3]. Magnetic levitational assembly of cells provides rapid, simple, cost-effective 3D cell culture formation while ensuring scaffold-free microenvironment. Here, our efforts are summarized in designing new magnetic levitation platform and biofabrication of 3D cellular entities via magnetic levitation for tissue engineering. Magnetic levitation and guidance of cells were provided by using a paramagnetic agent to fabricate scaffold-free 3D cellular structures. The parameters of cell density, paramagnetic agent concentration, and culturing time were optimized to obtain 3D cardiac cellular structures with tunable size, circularity, and high cell viability. Cellular and extracellular components of the 3D cellular structures were demonstrated via immunofluorescent staining. Also, 3D cardiac cellular structures showed more resistance to drug exposure compared to 2D control. In conclusion, MagLev methodology offers an easy and efficient way to fabricate 3D cellular structures for drug screening studies.
  • Conference Object
    Biopatterning of 3d Cellular Structures Via Contactless Magnetic Manipulation for Drug Screening
    (Mary Ann Liebert, 2023) Önbaş, Rabia; Arslan Yıldız, Ahu
    "Patterning and manipulation techniques have been used to fabricate 3D cell cultures in tissue engineering. The contactless magnetic manipulation approach is a rapid, simple, and cost-effective method that requires paramagnetic agents [1-3] or magnetic materials [4]. Here, to obtain patterned 3D cellular structures a new alginate-based bio-ink formulation was developed to fabricate 3D cellular structures using contactless magnetic manipulation. 3D cardiac model was obtained by patterning rat cardiomyocytes. Cellular and extracellular components and cardiac-specific markers of patterned 3D cellular structures were indicated successfully. Drug response of patterned 3D cellular structures was evaluated by applying doxorubicin. Patterned 3D cardiac cellular structures showed significantly different drug response compared to conventional 2D cell cultures. In conclusion, this technique provides an easy, efficient, and low-cost methodology to fabricate 3D cardiac structures for drug screening.