Bioengineering / Biyomühendislik
Permanent URI for this collectionhttps://hdl.handle.net/11147/4529
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Conference Object Citation - WoS: 1The Effects of Novel Telomerase Activators on Human Adipose-Derived Mesenchymal Stem Cell (had-Msc) Proliferation and Osteogenic Differentiation(Georg Thieme Verlag Kg, 2022) Kuru, G.; Küçüksolak, Melis; Pulat, G.; Karaman, O.; Bedir, Erdal[No Abstract Available]Conference Object Citation - WoS: 1Secondary Metabolites From Endophytic Fungus Penicilium Roseopurpureum and Investigation of Their Cytotoxic Activities(Georg Thieme Verlag, 2022) Dizmen, Berivan; Üner, Göklem; Küçüksolak, Melis; Ballar Kırmızıbayrak, Petek; Bedir, Erdal[No Abstract Available]Conference Object Citation - WoS: 1Phytochemical Studies on Mastic Gum of Pistacia Lentiscus Var. Chia Collected From Karaburun Peninsula and Neuroprotective Activities of the Isolates(Georg Thieme Verlag, 2022) Demir, Mehmet; Üner, Göklem; Mu, Kurt; Aygün, M.; Ballar Kırmızıbayrak, Petek; Bedir, Erdal[No Abstract Available]Conference Object Short Lecture "method Development for Pilot Production of Astragaloside Vii"(Georg Thieme Verlag, 2022) Kurt, Mustafa Ünver; Tağ, Özgür; Bedir, ErdalBased on the promising immunostimulant effect comparable to commercialized adjuvants Alum and Quillaja saponins (including QS-21) [1], [2], [3], our team has been prompted to carry out advance studies for developing Astragaloside VII (AST VII) ([Fig. 1]) as a new vaccine adjuvant or an immunotherapeutic agent. Hence, one of the most critical challenges is establishing efficient isolation and purification processes to obtain AST VII on a large scale. Thus, this study aimed to develop a production methodology for AST VII from Turkish Astragalus species.Conference Object Short Lecture Novel Neuroprotective Metabolites Produced Via Biotransformation of Cyclocephagenol by Alternaria Eureka 1e1bl1(Georg Thieme Verlag, 2022) Küçüksolak, Melis; Üner, Göklem; Ballar Kırmızıbayrak, Petek; Bedir, ErdalNeurodegeneration refers to the loss of structure/function of neurons leading to neurological diseases including Alzheimerʼs and Parkinsonʼs. The discovery of novel therapeutics against neurodegenerative diseases has been an area of intense research as neurodegenerative diseases are a huge burden on society and the economy [1]. Numerous studies reported that natural products have the potential to prevent and treat neurodegeneration. Among these studies, the neuroprotective activities of cycloartane-type saponins are noteworthy [2], [3]. In our preliminary studies, the neuroprotective activity of cyclocephagenol, an aglycone of cyclocephaloside I from Astragalus microcephalus [4], was screened for H2O2-induced injury in SH-SY5Y cells. Based on the promising bioactivity of cyclocephagenol, the aims of this study were: i) to perform microbial transformation studies on cyclocephagenol using Alternaria eureka followed by isolation and structural characterization of the metabolites; ii) to investigate neuroprotective activities of the metabolites; iii) to understand structure-activity relationships towards neuroprotection.Conference Object A New Iminol Derivative From Streptomyces Cacaoi in New Fermentation Conditions(Georg Thieme Verlag, 2022) Gezer, Emre; Küçüksolak, Melis; Bilgi, Eyüp; Bedir, ErdalMarine-derived organisms have varied secondary metabolism due to their adaptation to extreme conditions of marine environments. This fact has made marine-derived Actinobacteria promising sources of new/novel compounds. In addition, the expression of secondary metabolite gene clusters is typically under the control of environmental conditions that cause many of the biosynthetic gene clusters to be silent under laboratory conditions. Thus, the determination of proper fermentation conditions becomes crucial for discovering new molecules.Article Citation - WoS: 1Citation - Scopus: 1Exploring the Heterogeneity of Ige-Mediated Food Allergy Through Latent Class Analysis(S. Karger AG, 2022) Akarsu, Ayşegül; Öksel Karakuş, Ceyda; Ocak, Melike; Oral, Nihan; Bilgi, Eyüp; Şahiner, Ümit Murat; Soyer, Özge; Şekerel, Bülent EnisIntroduction: Food allergy (FA) is a heterogeneous disease with multiple morbidities and a huge burden for patients and healthcare systems. Variable manifestations, comorbidities (atopic dermatitis [AD], asthma, and/or allergic rhinitis [AR]), severity (anaphylaxis), and outcomes suggest the existence of different endotypes that cluster analyses may reveal. In this study, we aimed to investigate distinct subgroups among patients with FAs using data from 524 children/adolescents. Methods: 524 patients with IgE-mediated FA (353 male [67%]; median age 4.4 years [IQR:3.0-6.8]), 354 (68%) had multiple FA. The history of AD, asthma, AR, and anaphylaxis was recorded in 59.4%, 35.5%, 24.2%, and 51.2% of the patients, respectively. Latent class analysis was carried out to distinguish clinical FA phenotypes using five potential markers of allergy severity (single/multiple FA, never/inactive/current asthma and AD, AR, and anaphylaxis). Results: Three distinct phenotypes were identified: (1) multiple FA with eczema and respiratory multimorbidity (42%), (2) multiple FA with persistent eczema (34%), and (3) single FA with respiratory multimorbidity without eczema (24%). Compared with the single FA cluster, the prevalence of AD was significantly higher in multiple FA groups. Cluster 1 had the highest frequency of AR and allergic asthma, and the lowest rate of total tolerance of FA. Discussion: We put forward the hypothesis of underlying pathogenesis according to the clinical phenotypes. While skin barrier defect may play a dominant role in the pathogenesis in Cluster 2, immune dysregulation may be dominant in Cluster 3. In Cluster 1, the most severe group, a combination of both skin barrier defects and immune dysregulation may be responsible for the clinical features.Conference Object Citation - WoS: 1Microbial Transformation of Ruscogenins by Cunninghamella Blakesleeana(Georg Thieme Verlag, 2016) Özçınar, Özge; Tağ, Özgür; Kıvçak, Bijen; Bedir, ErdalThe natural product drug discovery process involves the isolation of new molecules from natural sources, investigation of their biological activities, and semi-synthesis of more active analogs. Microbial transformation plays a vital role in the preparation of new oxygenated derivatives, and has frequently been used as microbial model of mammalian drug metabolism [1,2]. It has been proved that the hydroxylation of steroidal compounds is catalyzed by cytochrome P450 monoxygenase systems, which exist in all eucaryotic microorganisms [3]. Cunninghamella genus has been widely used in transformation of steroids [4,5]. The major steroidal saponins of Ruscus aculeatus, ruscogenin and neoruscogenin, has strong anti-inflammatory activities, acts as an anti-elastase, and decreases capillary permeability [6]. In the present study microbial transformation of Neoruscogenin:Ruscogenin (78:22) mixture by Cunninghamella blakesleeana fungus afforded three new compounds. The structures were elucidated by LC-MS, 1D- and 2D NMR analyses as shown below. Mainly oxydation products were obtained from neoruscogenin by C. blakesleana. As far as can be ascertained from the literature, this is the first microbial transformation study performed on neoruscogenin.Conference Object Role of Connexin 32 on Gap Junctions in Breast Cancer Cells With Varying Metastatic Potential.(American Society for Cell Biology, 2017) Uğur, Deniz; Özçivici, Engin; Meşe, Gülistan[No abstract available]Conference Object Bioassay Guided Isolation of Naphthoquinones From Onosma Aksoyii, Investigation of Their Cytotoxic Properties(Georg Thieme Verlag, 2019) Kul, Demet; Karakoyun, Çiğdem; Yılmaz, Sinem; Pirhan, Ademi Fahri; Bedir, ErdalThe genus Onosma L. (Boraginaceae) includes about 230 species, distributed mainly in the Mediterranean region and Central Asia. Major constituents of Onosma species are alkaloids, naphthoquinones, polyphenols, phytosterols, terpenoids and fatty acids [1], [2]. Naphthoquinones are naturally widespread secondary metabolites deriving from some higher plants, fungi and bacteria. They exhibit significant biological activities such as cytotoxicity, antimalarial, antibacterial, antifungal and wound healing [2], [3]. Recently naphthoquinone derivatives have also been recognized as potent topoisomerase inhibitors [4].