Bioengineering / Biyomühendislik
Permanent URI for this collectionhttps://hdl.handle.net/11147/4529
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Conference Object Immobilized Gold Nanoparticle Based Plasmonic Assay Platform for Biomolecule and Microorganism Detection(Wiley, 2021) Sözmen, Alper Baran; Arslan Yıldız, AhuPlasmonic sensors are suitable tools for study of molecular interactions. Localized Surface Plasmon Resonance (LSPR) based sensors detect spectral changes associated with intramolecular interactions between analyte molecules and recognition elements. Due to its labelfree and highly sensitive features, LSPR based methods have high potential for biosensing applications. In this study, we aim to develop a sensitive, labelfree, rapid and simple biosensing platform. For this purpose, a novel refractive index (RI) sensitivity enhancement methodology is proposed by immobilizing gold nanoparticles (GNPs) for platformbased LSPR. Fabrication of platform was carried out by GNP synthesis, immobilization of GNPs on polystyrene solid support, and growth of GNPs. Validation of response to RI changes of developed sensor platform was carried out by tests with varying concentrations of sucrose and ethanol. Then as a proofofconcept, detection ability and detection limit determination of E.coli BL21 (DE3) and protein Bovine Serum Albumin (BSA) was carried out. Adsorption of E.coli BL21 (DE3) via bulk interactions showed that the developed LSPR platform exhibit high enough binding affinity for bacteria detection, and was able to detect down to concentrations as low as 102 CFU/ml. Immune capturing of BSA via antiBSA antibody showed that the developed LSPR platform was able to detect BSA protein–antibody interaction down to 10 µM concentration range.Article Citation - WoS: 27Citation - Scopus: 28Biocomposite Scaffolds for 3d Cell Culture: Propolis Enriched Polyvinyl Alcohol Nanofibers Favoring Cell Adhesion(John Wiley and Sons Inc., 2021) Bilginer, Rumeysa; Özkendir İnanç, Dilce; Yıldız, Ümit Hakan; Arslan Yıldız, AhuThe objective of this work is generation of propolis/polyvinyl alcohol (PVA) scaffold by electrospinning for 3D cell culture. Here, PVA used as co-spinning agent since propolis alone cannot be easily processed by electrospinning methodology. Propolis takes charge in maximizing biological aspect of scaffold to facilitate cell attachment and proliferation. Morphological analysis showed size of the electrospun nanofibers varied between 172-523 nm and 345-687 nm in diameter, for non-crosslinked and crosslinked scaffolds, respectively. Incorporation of propolis resulted in desired surface properties of hybrid matrix, where hybrid scaffolds highly favored protein adsorption. To examine cell compatibility, NIH-3T3 and HeLa cells were seeded on propolis/PVA hybrid scaffold. Results confirmed that integration of propolis supported cell adhesion and cell proliferation. Also, results indicated electrospun propolis/PVA hybrid scaffold provide suitable microenvironment for cell culturing. Therefore, developed hybrid scaffold could be considered as potential candidate for 3D cell culture and tissue engineering.Article Citation - WoS: 21Citation - Scopus: 16A Facile Method To Fabricate Propolis Enriched Biomimetic Pva Architectures by Co-Electrospinning(Elsevier Ltd., 2020) Bilginer, Rümeysa; Arslan Yıldız, AhuThis study depicts easy process of propolis by co-electrospinning without using any toxic agent for biomedical applications. To achieve this, polyvinyl alcohol was utilized as co-spinning agent to fabricate biomimetic Propolis/PVA scaffold. Here, whilst PVA was used as a supportive material to accumulate propolis in scaffold, propolis was employed to enrich biologic aspect of scaffold. This strategy overcomes challenges of propolis processing originated from solubility problems and offers easy processability of propolis in order to use in biomedical applications. Electrospun Propolis/PVA scaffolds were crosslinked with glutaraldehyde and drop-cast model was utilized as a control. Formation of porous, bead-free nanofiber architectures was confirmed through surface morphology analysis, while drop-cast model shows non-porous morphology. Wettability results confirmed both crosslinking and integration of propolis into polyvinyl alcohol scaffold moved contact angle to hydrophobic region. Presence and amount of propolis in hybrid scaffolds were validated via absorbance spectrum results. Bioactivity and biocompatibility of propolis-enriched scaffolds were analyzed through protein adsorption capacity. Obtained findings are evidence that electrospinning methodology offers easy and biosafe process of propolis. Electrospun Propolis/PVA exhibits desired properties and could be potentially utilized as scaffold for tissue engineering or as a wound dressing graft in biomedical field. © 2020 Elsevier B.V.