Bioengineering / Biyomühendislik

Permanent URI for this collectionhttps://hdl.handle.net/11147/4529

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Institution Author: search.filters.institutionAuthor.Karakuzu, Betül

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Now showing 1 - 4 of 4
  • Article
    Citation - WoS: 15
    Citation - Scopus: 16
    Electromechanical Rt-Lamp Device for Portable Sars-Cov Detection
    (Elsevier, 2023) Tarım, Ergün Alperay; Öksüz, Cemre; Tekin, Hüseyin Cumhur; Appak, Özgür; Sayıner, Ayça Arzu; Tekin, Hüseyin Cumhur; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    Rapid point-of-care tests for infectious diseases are essential, especially in pandemic conditions. We have developed a point-of-care electromechanical device to detect SARS-CoV-2 viral RNA using the reverse-transcription loop-mediated isothermal amplification (RT-LAMP) principle. The developed device can detect SARS-CoV-2 viral RNA down to 103 copies/mL and from a low amount of sample volumes (2 μL) in less than an hour of standalone operation without the need for professional labor and equipment. Integrated Peltier elements in the device keep the sample at a constant temperature, and an integrated camera allows automated monitoring of LAMP reaction in a stirring sample by using colorimetric analysis of unfocused sample images in the hue/saturation/value color space. This palm-fitting, portable and low-cost device does not require a fully focused sample image for analysis, and the operation could be stopped automatically through image analysis when the positive test results are obtained. Hence, viral infections can be detected with the portable device produced without the need for long, expensive, and labor-intensive tests and equipment, which can make the viral tests disseminated at the point-of-care.
  • Article
    Citation - WoS: 10
    Citation - Scopus: 14
    An Electromechanical Lab-On Platform for Colorimetric Detection of Serum Creatinine
    (American Chemical Society, 2022) Karakuzu, Betül; Tarım, Ergün Alperay; Tekin, Hüseyin Cumhur; Tekin, Hüseyin Cumhur; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    Chronic kidney disease (CKD) is a high-cost disease that affects approximately one in ten people globally, progresses rapidly, results in kidney failure or dialysis, and triggers other diseases. Although clinically used serum creatinine tests are used to evaluate kidney functions, these tests are not suitable for frequent and regular control at-home settings that obstruct the regular monitoring of kidney functions, improving CKD management with early intervention. This study introduced a new electromechanical lab-on-a-chip platform for point-of-care detection of serum creatinine levels using colorimetric enzyme-linked immunosorbent assay (ELISA). The platform was composed of a chip containing microreservoirs, a stirring bar coated with creatinine-specific antibodies, and a phone to detect color generated via ELISA protocols to evaluate creatinine levels. An electromechanical system was used to move the stirring bar to different microreservoirs and stir it inside them to capture and detect serum creatinine in the sample. The presented platform allowed automated analysis of creatinine in ~50 min down to ~1 and ~2 mg/dL in phosphate-buffered saline (PBS) and fetal bovine serum (FBS), respectively. Phone camera measurements in hue, saturation, value (HSV) space showed sensitive analysis compared to a benchtop spectrophotometer that could allow low-cost analysis at point-of-care.
  • Conference Object
    Electromechanical Lab-On Platform for Creatinine Analysis Using Automated Elisa Protocols
    (Chemical and Biological Microsystems Society, 2020) Karakuzu, Betül; Tarım, Ergün Alperay; Tekin, Hüseyin Cumhur; Tekin, Hüseyin Cumhur; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    We present an electromechanical lab-on-a-chip (LOC) platform for the automated serum creatinine detection applying enzyme-linked immunosorbent assay (ELISA) principle. In the platform, antibody covered bar selectively captures the creatinine in the sample and the electromechanical system allows automatic movement between the designed reservoirs containing assay solutions. At the end of the protocol, the absorbance value of the appeared color is measured to determine creatinine concentration in the sample. Since this system allows measuring automatically creatinine levels with minimum time and cost, it can be utilized for point-of-care monitoring of chronic kidney diseases (CKD) for the future. © 2020 CBMS-0001
  • Conference Object
    Citation - WoS: 3
    Citation - Scopus: 3
    Active Mixing Strategy With Electromechanical Platform for Lab-On Applications
    (Institute of Electrical and Electronics Engineers Inc., 2019) Karakuzu, Betül; Tekin, Hüseyin Cumhur; Özçivici, Engin; Özçivici, Engin; Tekin, Hüseyin Cumhur; Tarım, E. Alperay; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of Technology
    The main purpose of this study is to present a new active mixing strategy that can be used for lab-on-A-chip applications to shorten analysis time. An electromechanical platform composed of stepper and DC motors is designed and manufactured. This platform allows rapid mixing in microwells of a polydimethylsiloxane chip for analysis. Mixing in microwells is performed with a stirring bar spun automatically using the electromechanical platform. Mixing experiments performed at different spinning speeds and different time intervals on the platform. It was observed that mixing was achieved only in 300 ms inside 100 ?L microwell using 4300 revolutions per minute (rpm) spinning speeds. Hence, the proposed mixing strategy showed 200-fold faster mixing than pure diffusion-based mixing. © 2019 IEEE.