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Citation - WoS: 34
Citation - Scopus: 34
Decellularised Extracellular Matrix Decorated Pcl Polyhipe Scaffolds for Enhanced Cellular Activity, Integration and Angiogenesis
(Royal Society of Chemistry, 2021) Dikici, Serkan; Aldemir Dikici, Betül; MacNeil, Sheila; Claeyssens, Frederik
Wound healing involves a complex series of events where cell-cell and cell-extracellular matrix (ECM) interactions play a key role. Wounding can be simple, such as the loss of the epithelial integrity, or deeper and more complex, reaching to subcutaneous tissues, including blood vessels, muscles and nerves. Rapid neovascularisation of the wounded area is crucial for wound healing as it has a key role in supplying oxygen and nutrients during the highly demanding proliferative phase and transmigration of inflammatory cells to the wound area. One approach to circumvent delayed neovascularisation is the exogenous use of pro-angiogenic factors, which is expensive, highly dose-dependent, and the delivery of them requires a very well-controlled system to avoid leaky, highly permeable and haemorrhagic blood vessel formation. In this study, we decorated polycaprolactone (PCL)-based polymerised high internal phase emulsion (PolyHIPE) scaffolds with fibroblast-derived ECM to assess fibroblast, endothelial cell and keratinocyte activity in vitro and angiogenesis in ex ovo chick chorioallantoic membrane (CAM) assays. Our results showed that the inclusion of ECM in the scaffolds increased the metabolic activity of three types of cells that play a key role in wound healing and stimulated angiogenesis in ex ovo CAM assays over 7 days. Herein, we demonstrated that fibroblast-ECM functionalised PCL PolyHIPE scaffolds appear to have great potential to be used as an active wound dressing to promote angiogenesis and wound healing.
Citation - WoS: 2
Citation - Scopus: 2
Correction: Scaffold-Free Three-Dimensional Cell Culturing Using Magnetic Levitation
(Royal Society of Chemistry, 2018) Türker, Esra; Demircak, Nida; Arslan Yıldız, Ahu
The authors regret the inclusion of an incorrect figure caption for Fig. 2. The corrected figure caption for Fig. 2 is shown below. Fig. 2 Evaluation of levitation height (z) and density profiles through magnetic levitation. (A) Gd(III) chelates were named as Gx (Gadovist/Gadobutrol), Dx (Dotarem/Gadoteric acid) and Ox (Omniscan/Gadodiamide). (B) Standard curve for PE bead density against levitation height; linear curve fitting gives the standard function for the corresponding curve. (C–E) Levitation height profiles of single NIH 3T3 cells under 30/50/100/200 mM Gd concentrations. Single cell density profiles calculated through standard function of linear fitting.
Citation - WoS: 75
Citation - Scopus: 74
Scaffold-Free Three-Dimensional Cell Culturing Using Magnetic Levitation
(Royal Society of Chemistry, 2018) Türker, Esra; Demirçak, Nida; Arslan Yıldız, Ahu
Three-dimensional (3D) cell culture has emerged as a pioneering methodology and is increasingly utilized for tissue engineering, 3D bioprinting, cancer model studies and drug development studies. The 3D cell culture methodology provides artificial and functional cellular constructs serving as a modular playground prior to animal model studies, which saves substantial efforts, time and experimental costs. The major drawback of current 3D cell culture methods is their dependency on biocompatible scaffolds, which often require tedious syntheses and fabrication steps. Herein, we report an easy-to-use methodology for the formation of scaffold-free 3D cell culture and cellular assembly via magnetic levitation in the presence of paramagnetic agents. To paramagnetize the cell culture environment, three different Gadolinium(iii) chelates were utilized, which led to levitation and assembly of cells at a certain levitation height. The assembly and close interaction of cells at the levitation height where the magnetic force was equilibrated with gravitational force triggered the formation of complex 3D cellular structures. It was shown that Gd(iii) chelates provided an optimal levitation that induced intercellular interactions in scaffold-free format without compromising cell viability. NIH 3T3 mouse fibroblasts and HCC827 non-small-cell lung cancer cells were evaluated via the magnetic levitation system, and the formation of 3D cell culture models was validated for both cell lines. Hereby, the developed magnetic levitation system holds promises for complex cellular assemblies and 3D cell culture studies.

Bioengineering / Biyomühendislik

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