Bioengineering / Biyomühendislik
Permanent URI for this collectionhttps://hdl.handle.net/11147/4529
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Conference Object A Glucuronoxylan-Based Bio-Ink Development: Characterization and Application(Wiley, 2023) Yıldırım, Ömer; Arslan Yıldız, AhuBioprinting is a trending technique that enables the fabrication of threedimensional (3D) constructs in designed shapes and with desired properties. Bioinks are one of the most significant components of bioprinting and the successful fabrication of 3D bioprinted constructs mostly depends on the features of bioinks that would be used. New generation bioinks that are soft and viscous enough, printable under low pressure, stable in cell culture, and have fast gelation mechanisms are ideal to be used in current bioprinting techniques. Hydrocolloids have said features and have similar properties to native ECM structures. Hence bioinks that are developed from hydrocolloids can be utilized for mimicking of ECM structure of soft tissues. Polysaccharidebased hydrocolloids are ideal bioink candidates with their high waterholding capacity and biocompatibility. Here, a glucuronoxylanbased newgeneration bioink was developed, and its printability was evaluated for 3D bioprinting applications. The glucuronoxylanbased hydrocolloid was obtained by water extraction of quince seeds and its utilization in bioprinting was investigated. Bioink characterization was done by FTIR and mechanical analysis. Bioprinting parameters were optimized assessing uniformity, pore factor, and shape fidelity. Then, the characterization of bioprinted constructs was performed by pore angle measurement, waterholding capacity analysis, protein adsorption, and cell viability assays. Bioprinted structures have high mechanical strength, suitable protein adsorption behavior, and waterholding capacity as high as 20fold of its own weight, which is higher than other hydrogels that were used in soft tissue engineering. Moreover, the cell viability results of fibroblast cells in the bioink were high for longterm culture. In conclusion, findings show that the developed glucuronoxylanbased bioink is a biocompatible and promising bioink material for further tissue engineering applications.Conference Object Biopatterning of 3d Cellular Structures Via Contactless Magnetic Manipulation for Drug Screening(Mary Ann Liebert, 2023) Onbas, Rabia; Arslan Yıldız, AhuAnnotation On-Chip 3d Cell Culture Platform for Tumor Modeling and Drug Screening(2022) Yıldırım, Özüm; Arslan Yıldız, AhuArticle Citation - WoS: 3Citation - Scopus: 4Biopatterning of 3d Cellular Model by Contactless Magnetic Manipulation for Cardiotoxicity Screening(Mary Ann Liebert, Inc, 2023) Önbaş, Rabia; Arslan Yıldız, AhuPatterning cells to create three-dimensional (3D) cell culture models by magnetic manipulation is a promising technique, which is rapid, simple, and cost-effective. This study introduces a new biopatterning approach based on magnetic manipulation of cells with a bioink that consists alginate, cells, and magnetic nanoparticles. Plackett-Burman and Box-Behnken experimental design models were used to optimize bioink formulation where NIH-3T3 cells were utilized as a model cell line. The patterning capability was confirmed by light microscopy through 7 days culture time. Then, biopatterned 3D cardiac structures were formed using H9c2 cardiomyocyte cells. Cellular and extracellular components, F-actin and collagen Type I, and cardiac-specific biomarkers, Troponin T and MYH6, of biopatterned 3D cardiac structures were observed successfully. Moreover, Doxorubicin (DOX)-induced cardiotoxicity was investigated for developed 3D model, and IC50 value was calculated as 8.1 μM for biopatterned 3D cardiac structures, which showed higher resistance against DOX-exposure compared to conventional two-dimensional cell culture. Hereby, developed biopatterning methodology proved to be a simple and rapid approach to fabricate 3D cardiac models, especially for drug screening applications. Copyright 2023, Mary Ann Liebert, Inc., publishers.Conference Object Biofabrication of Scaffold-Free 3d Cellular Structures Using Magnetic Levitational Assembly To Study Cardiac Toxicity(Mary Ann Liebert, 2023) Yıldız, Ahu Arslan; Arslan Yıldız, Ahu; Onbaş, RabiaSpheroids are one of the well-characterized 3D cell culture approaches for drug screening and therapeutic studies. Magnetic levitation (MagLev) is a newly developing approach to form 3D cellular structures and spheroids [1,2,3]. Magnetic levitational assembly of cells provides rapid, simple, cost-effective 3D cell culture formation while ensuring scaffold-free microenvironment. Here, our efforts are summarized in designing new magnetic levitation platform and biofabrication of 3D cellular entities via magnetic levitation for tissue engineering. Magnetic levitation and guidance of cells were provided by using a paramagnetic agent to fabricate scaffold-free 3D cellular structures. The parameters of cell density, paramagnetic agent concentration, and culturing time were optimized to obtain 3D cardiac cellular structures with tunable size, circularity, and high cell viability. Cellular and extracellular components of the 3D cellular structures were demonstrated via immunofluorescent staining. Also, 3D cardiac cellular structures showed more resistance to drug exposure compared to 2D control. In conclusion, MagLev methodology offers an easy and efficient way to fabricate 3D cellular structures for drug screening studies.Book Part Citation - Scopus: 2Bioprinting of Hydrogels for Tissue Engineering and Drug Screening Applications(Elsevier, 2022) Özmen, Ece; Yıldırım, Özüm; Arslan Yıldız, AhuIn tissue engineering, the 3-dimensional (3D) bioprinting method that enables the production of 3D structures by combining bioinks and cells has become one of the most promising technique. Over the last few years, 3D cell culture models gained importance in the development of disease model and drug development studies. The successful production of the 3D structures by 3D bioprinting mostly depends on the properties of the bioink to be used. Hydrogels, which are natural or synthetic polymers, are generally preferred as bioink materials with their high swelling ability, biocompatibility, biodegradability, and easy gelation ability. The convenience of hydrogels for varied bioprinting applications make them proper bioink materials for bioprinting of artificial tissues, tumor models, and tissue grafts. Bioprinting of functional tissues is successfully performed for years, and hydrogels are utilized as bioink in bone, vascular, neural, cartilage, cardiac, skin tissue engineering, and drug screening. In this chapter, bioprinting methodology, bioinks, hydrogel bioinks, and their applications are discussed in detail. © 2023 Elsevier Inc. All rights reserved.Book Part Citation - Scopus: 3Tissue Engineering Applications of Marine-Based Materials(Springer, 2022) Polat, Hürriyet; Zeybek, Nuket; Polat, MehmetTissue engineering is a promising approach in replacing or improving tissues lost or has become nonviable due to disease or trauma by the use of scaffold materials by combining engineering and biochemical/physicochemical methods. Its purpose is to create suitable matrices that support cell differentiation and proliferation toward the formation of new and functional tissue. Marine-based natural compounds are potential scaffold feedstock material in tissue engineering owing to their biocompatibility and biodegradability while providing excellent biochemical/physicochemical properties. Numerous application areas and various fabrication routes techniques described in the literature attest to the importance of these materials in tissue regeneration. This review has been carried to merge the information from a large number of studies on the marine-based scaffold materials in tissue engineering into a coherent summary. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2022.Conference Object Citation - WoS: 1The Effects of Novel Telomerase Activators on Human Adipose-Derived Mesenchymal Stem Cell (had-Msc) Proliferation and Osteogenic Differentiation(Georg Thieme Verlag Kg, 2022) Kuru, G.; Küçüksolak, Melis; Pulat, G.; Karaman, O.; Bedir, Erdal[No Abstract Available]Conference Object Citation - WoS: 1Secondary Metabolites From Endophytic Fungus Penicilium Roseopurpureum and Investigation of Their Cytotoxic Activities(Georg Thieme Verlag, 2022) Dizmen, Berivan; Üner, Göklem; Küçüksolak, Melis; Ballar Kırmızıbayrak, Petek; Bedir, Erdal[No Abstract Available]Conference Object Citation - WoS: 1Phytochemical Studies on Mastic Gum of Pistacia Lentiscus Var. Chia Collected From Karaburun Peninsula and Neuroprotective Activities of the Isolates(Georg Thieme Verlag, 2022) Demir, Mehmet; Üner, Göklem; Mu, Kurt; Aygün, M.; Ballar Kırmızıbayrak, Petek; Bedir, Erdal[No Abstract Available]