Bioengineering / Biyomühendislik

Permanent URI for this collectionhttps://hdl.handle.net/11147/4529

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2015 - 20183

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Author: search.filters.author.Tamburacı, SedefLanguage: search.filters.language.en

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  • Article
    Citation - Scopus: 21
    Determination of Superoxide Dismutase Activities in Different Cyanobacteria for Scavenging of Reactive Oxygen Species
    (Taylor and Francis Ltd., 2015) Güneş, Seda; Tamburacı, Sedef; İmamoğlu, Esra; Dalay, Meltem Conk
    There is considerable interest in cyanobacteria as sources of antioxidant pigments, antiinflammatory substances, enzymes such as superoxide dismutase (SOD) and vitamins. Antioxidant enzymes have significant role in the removal of reactive oxygen species produced during visible and ultraviolet irradiance stress in cyanobacteria. One of the most ubiquitous protective enzyme is superoxide dismutase (SOD). Most of cyanobacteria are known to have both FeSOD and MnSOD which are associated with photosystem I and II. These enzymes are responsible for scavenging superoxide radicals.Spirulina platensis and Synechococcus nidulans are widely used cyanobacteria as food supplement and cosmetic due to their bioactive compounds. Although the chemical composition of S.platensis has been investigated by many researchers, research on the relationship between protein content and comparison of SOD activity with other cyanobacteria is limited.Among several SOD producing cyanobacteria, the most promising organisms were investigate in terms of SOD activities in this study. The aim of the present work was to investigate and compare the SOD activities of different cyanobacteria including S. platensis, Pseudanabeana sp., S. nidulans and associate with protein contents. SOD activities of these cyanobacteria were determined by xanthine/xanthineoxidase method.Modified Lowry method was used for protein determination. The analysis results showed that the maximum specific SOD activity obtained was 50.4 U/mg from S.nidulans and the minimum specific SOD activity was 18.4 U/mg from Pseudanabeana sp. Total protein amount of S. nidulans, S. platensis, Pseudanabeana sp. was determined as 0.25 mg/ml, 0.27 mg/ml and 0.43 mg/ml respectively. As a result, Synechococcus nidulans was more efficient at scavenging peroxide radicals than Pseudanabeana sp. © 2015, © 2015 Har Krishan Bhalla & Sons.
  • Article
    Citation - WoS: 73
    Citation - Scopus: 94
    In Vitro Evaluation of Spirulina Platensis Extract Incorporated Skin Cream With Its Wound Healing and Antioxidant Activities
    (Taylor & Francis, 2017) Güneş, Seda; Tamburacı, Sedef; Conk Dalay, Meltem; Deliloğlu Gürhan, İsmet
    Context: Algae have gained importance in cosmeceutical product development due to their beneficial effects on skin health and therapeutical value with bioactive compounds. Spirulina platensis Parachas (Phormidiaceae) is renowned as a potential source of high-value chemicals and recently used in skincare products. Objective: This study develops and evaluates skin creams incorporated with bioactive S. platensis extract. Materials and methods:Spirulina platensis was cultivated, the aqueous crude extract was prepared and in vitro cytotoxicity of S. platensis extract in the range of 0.001-1% concentrations for 1, 3 and 7d on HS2 keratinocyte cells was determined. Crude extracts were incorporated in skin cream formulation at 0.01% (w/w) concentration and in vitro wound healing and genotoxicity studies were performed. Immunohistochemical staining was performed to determine the collagen activity. Results: 0.1% S. platensis extract exhibited higher proliferation activity compared with the control group with 198% of cell viability after 3 d. Skin cream including 1.125% S. platensis crude extract showed enhanced wound healing effect on HS2 keratinocyte cell line and the highest HS2 cell viability % was obtained with this concentration. The micronucleus (MN) assay results indicated that S. platensis extract incorporated creams had no genotoxic effect on human peripheral blood cells. Immunohistochemical analysis showed that collagen 1 immunoreactivity was improved by increased extract concentration and it was strongly positive in cells treated with 1.125% extract incorporated skin cream. Conclusions: The cell viability, wound healing activity and genotoxicity results showed that S. platensis incorporated skin cream could be of potential value in cosmeceutical and biomedical applications.
  • Article
    Citation - WoS: 25
    Citation - Scopus: 33
    Novel Phytochemical Cissus Quadrangularis Extract-Loaded Chitosan/Na-carboxymethyl Cellulose-Based Scaffolds for Bone Regeneration
    (SAGE Publications, 2018) Tamburacı, Sedef; Kimna, Ceren; Tıhmınlıoğlu, Funda
    Medicinal plants are attracting considerable interest as a potential therapeutic agent for bone tissue regeneration. Cissus quadrangularis L. is also a medicinal plant known with its osteogenic activity. In this study, a phytochemical scaffold was produced by incorporating Cissus quadrangularis with chitosan/Na-carboxymethyl cellulose blend by lyophilization technique. The effect of Cissus quadrangularis loading on the mechanical, morphological, chemical, and degradation properties as well as in vitro cytotoxicity, cell proliferation, and differentiation of the composites was investigated. Scanning electron microscopy images showed that porous Cissus quadrangularis-loaded scaffolds were obtained with an average pore size of 148-209 mu m which is appropriate for bone regeneration. Cissus quadrangularis incorporation enhanced the compression modulus of scaffolds from 76 to 654 kPa. In vitro cell culture results indicated that Cissus quadrangularis/chitosan/Na-carboxymethyl cellulose scaffolds provided a favorable substrate for the osteoblast adhesion, proliferation, and mineralization. Results supported the osteoinductive property of the Cissus quadrangularis extract-incorporated scaffolds even without osteogenic media supplement. Cissus quadrangularis extract increased the alkaline phosphatase activity of the SaOS-2 cells on scaffolds on 7th and 14th days of incubation. The investigation of characterization and cell culture studies suggest that Cissus quadrangularis-loaded osteoinductive Cissus quadrangularis/chitosan/Na-carboxymethyl cellulose scaffold can serve as a potential biomaterial for bone tissue engineering applications.