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Citation - WoS: 30
Citation - Scopus: 33
Molecular Separation by Using Active and Passive Microfluidic Chip Designs: a Comprehensive Review
(Wiley, 2023) Ebrahimi, Aliakbar; Didarian, Reza; Shih, Chih-Hsin; Nasseri, Behzad; Ethan Li, Yi-Chen; Shih, Steven; İçöz, Kutay; Tarım, Ergün Alperay; Akpek, Ali; Çeçen, Berivan; Bal Öztürk, Ayça; Güleç, Kadri; Tarım, Burcu Sırma; Tekin, Hüseyin Cumhur
Separation and identification of molecules and biomolecules such as nucleic acids, proteins, and polysaccharides from complex fluids are known to be important due to unmet needs in various applications. Generally, many different separation techniques, including chromatography, electrophoresis, and magnetophoresis, have been developed to identify the target molecules precisely. However, these techniques are expensive and time consuming. “Lab-on-a-chip” systems with low cost per device, quick analysis capabilities, and minimal sample consumption seem to be ideal candidates for separating particles, cells, blood samples, and molecules. From this perspective, different microfluidic-based techniques have been extensively developed in the past two decades to separate samples with different origins. In this review, “lab-on-a-chip” methods by passive, active, and hybrid approaches for the separation of biomolecules developed in the past decade are comprehensively discussed. Due to the wide variety in the field, it will be impossible to cover every facet of the subject. Therefore, this review paper covers passive and active methods generally used for biomolecule separation. Then, an investigation of the combined sophisticated methods is highlighted. The spotlight also will be shined on the elegance of separation successes in recent years, and the remainder of the article explores how these permit the development of novel techniques. © 2023 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.
Electromechanical Lab-On Platform for Creatinine Analysis Using Automated Elisa Protocols
(Chemical and Biological Microsystems Society, 2020) Karakuzu, Betül; Tarım, Ergün Alperay; Öksüz, Cemre; Tekin, Hüseyin Cumhur
We present an electromechanical lab-on-a-chip (LOC) platform for the automated serum creatinine detection applying enzyme-linked immunosorbent assay (ELISA) principle. In the platform, antibody covered bar selectively captures the creatinine in the sample and the electromechanical system allows automatic movement between the designed reservoirs containing assay solutions. At the end of the protocol, the absorbance value of the appeared color is measured to determine creatinine concentration in the sample. Since this system allows measuring automatically creatinine levels with minimum time and cost, it can be utilized for point-of-care monitoring of chronic kidney diseases (CKD) for the future. © 2020 CBMS-0001

Bioengineering / Biyomühendislik

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