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In Vitro Antitumor Activity of Sarcopoterium Spinosum Leaf Extract With Bioactive Natural Compounds
(Elsevier, 2013) Süngüç, Ceren; Erdoğan, İpek; Uslu, Mehmet Emin; Bayraktar, Oğuz
Cancer cell lines cause generation of reactive oxygen species and free radicals at high levels (Wang and Yi, 2008). Then generated free radicals lead to breakdown of the structure of DNA, lipid or protein (Gul et al., 2011). When plant extracts including antioxidant phytochemicals are exposed to the redox reactions, the harmful effects of free radicals are effectively prevented. The aim of present research was to evaluate the antitumor potential of the extract derived from Sarcopoterium spinosum leaves. The leaves of S. spinosum were collected in Izmir, Turkey. Total phenol content of ethanolic.
Investigation of Anti-Tumoral Activity of Cistus Creticus Extract Against Pc-3 Cell Line
(Elsevier, 2013) Erdoğan, İpek; Süngüç, Ceren; Uslu, Mehmet Emin; Bayraktar, Oğuz
Recent studies have revealed that plant extracts show cytotoxic activities against cancer cell lines by ceasing cell division in particular phases (Xu et al., 2012, Yıldırım et al., 2013). Expression of specific genes was found to be activated according to pathway in which cell death occurs. Objective of this study was to identify antitumoural effect of Cistus creticus, which is a perennial shrub, found in Mediterranean region, against prostate cancer cell line by measuring the cytotoxic activities and apoptotic gene expression levels.
Kinetic and Structural Characterization of Interaction Between Trypsin and Equisetum Arvense Extract
(Türk Biyokimya Derneği, 2014) Uslu, Mehmet Emin; Bayraktar, Oğuz; Ceylan, Çağatay
Objective: In this study the inhibitory effect of E. arvense extract on trypsin activity and the effect of trypsin on E. arvense extract were studied. In addition the nature of the interaction between the extract and trypsin was investigated. Methods: The inhibitory effect ethanol extract of E. arvense on trypsin activity was determined using trypsin enzyme assay. The structural effects of the extract-trypsin interaction for the extract were analyzed by FTIR. Finally, the HPLC analyses were carried out to analyze the individual components of the extract and the supernatant and soluble precipitate phases. Results: E. arvense extract was found to decrease total percent activity of trypsin to 5% in 24 hour at 24 °C. FTIR analyses indicated that the interaction between trypsin and E. arvense extract caused changes in the structure and hydrogen bonding behavior and composition of the extract proteins. These interactions also caused the extract lipids to accumulate in the insoluble precipitate phase. Most of the phenolics remained in the supernatant phase enhancing the inactivation of trypsin. However, the precipitated compounds were shown to be of apolar in nature as shown in the HPLC chromatograms. Conclusion: The methods that were used showed that the high phenolic content of E. arvense was the main reason for the inhibition of trypsin enzyme activity by denaturing the enzyme.
Citation - WoS: 22
Citation - Scopus: 24
Optimization of Extraction Conditions for Active Components in Equisetum Arvense Extract
(Ars Docendi, 2013) Uslu, Mehmet Emin; Erdoğan, İpek; Bayraktar, Oğuz; Ateş, Mehmet
Response surface methodology was applied to predict optimum conditions for equisetum arvense extraction. Central composite design was used to monitor the effects of temperature, stirring speed, ethanol percent, extraction time, solid-liquid ratio on dependent variables such as, extract yield percent, total phenol content, total antioxidant capacity, silicic acid amount. According to the mathematical models obtained from the analysis, the highest values for yield percent, total phenol content, total antioxidant capacity and silicic acid amount were found to be 18.67 %, 123 mg gallic acid gr-1 dry weight extract, 1608 μM TEAC mg-1 dry weight extract and 0.0049 mg silicic acid mg-1 dry weight extract, respectively. The plant extracts were analyzed with HPLC to determine the phenolic content and compositional differences of extracts obtained at different extraction conditions. Plant extracts were also analyzed for their cytotoxic and antimicrobial activities. The high total antioxidant capacity and total phenolic content resulted in an increased cytotoxic effect on fibroblast cells. Equisetum arvense extracts showed antimicrobial activity against Staphylococcus epidermidis and Escherichia coli bacteria, however showed no effect against Candida albicans. © 2013 University of Bucharest.

Bioengineering / Biyomühendislik

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