PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection

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  • Article
    Valorization of Recycled Waste in Green/White Purification and LC-QTOF/MS Analysis of Beverages Adulterated with Incapacitating Drugs
    (Elsevier B.V., 2026) Anilanmert, Beril; Yonar, Fatma Cavus; Er, Elif Ozturk; Pekcaliskan, Elif Yılmaz; Cengiz, Salih
    Incapacitating drugs constitute a growing threat for the community, since victims may drink adulterated beverages without noticing. A validated eco-friendly/economical purification/analysis kit prototype, along with an LC-QToF/MS method has been developed in coke and mixed fruit-juice, for simultaneous determination of 10 drugs used for incapacitating victims (zaleplone, zolpidem, zopiclone, mephedrone, fentanyl, phenytoin, thiopental, sertraline, ketamine and GHB). A combination of two different waste nut-shells which yielded the highest recovery for these drugs were directly used as adsorbent after grinding and modification and a reusable separation apparatus recycled from waste were utilized for the first time in a toxicological analysis. In the method, after adding the adsorbent on to the sample, pH was adjusted. Following 25-min (min) automatic vortexing for adsorption, matrix was removed easily, using the separation apparatus. After 25-min desorption via cold ultrasonication using 500 μL methanol, a 9.5-min LC-QToF/MS analysis was performed. The validated method in fruit-juice and coke, extraordinarily gave successful results also in urine and saliva. Assessment tools for greenness/whiteness and pictograms confirmed the environmental friendliness of the method kit. © 2025 Elsevier B.V.
  • Article
    FTIR Spectroscopy Coupled With Chemometrics for Evaluating Functional Food Efficacy in an in Vitro Model of Iron Deficiency Anemia
    (Elsevier Science Ltd, 2026) Dalyan, Eda; Cavdaroglu, Cagri; Ozen, Banu; Gulec, Sukru
    Vibrational spectroscopy offers a rapid, cost-effective approach for studying biological systems. This study employs Fourier Transform Infrared (FTIR) spectroscopy, combined with Soft Independent Modeling of Class Analogy (SIMCA), to evaluate treatment outcomes for iron deficiency anemia (IDA). The model was built using spectra from healthy and anemic cells, then validated with cells treated with commonly used iron supplements. In calibration, 9 of 10 control and all IDA samples were correctly classified; 14 of 15 validation samples were identified as healthy. The model was applied to cells treated with protein-iron complexes. All samples treated with a 60:1 protein-iron ratio matched the healthy group, while 3 of 4 treated with a 10:1 ratio matched the IDA group. These results were further supported by iron-regulated gene expression of transferrin receptor (TFR) and (Ankyrin Repeat Domain 37) ANKRD37. FTIR coupled with chemometrics enables rapid assessment of functional effects and shows potential for screening functional ingredients in anemia-targeted food products.
  • Article
    Chloroaluminum Phthalocyanine Loaded Bovine Serum Albumin Nanoparticles as a Dual-Functional Nanoplatform for Sono-Photodynamic Cancer Therapy
    (Elsevier, 2026) Akyol-Karpuzcu, Cansu; Nartas, Eylem Doga; Calibasi-Kocal, Gizem; Akdogan, Yasar
    Chloroaluminum phthalocyanine (ClAlPc) loaded bovine serum albumin (BSA) nanoparticles (NPs) were synthesized as a dual-functional platform for photodynamic and sonodynamic therapies (PDT and SDT). ClAlPc loading did not disturb the morphology of the BSA NPs. Their spherical structure, with a size around 200 nm, was preserved upon ClAlPc loading (1 %w/w). Singlet oxygen productions in the presence of ClAlPc loaded BSA NPs or free ClAlPc were determined by ultraviolet absorption (UV-vis) spectroscopy and electron paramagnetic resonance (EPR) spectroscopy. While a slower rate of singlet oxygen formation rate after both PDT and SDT was detected by UV-vis measurements in the presence of ClAlPc loaded BSA NPs, EPR results showed a similar rate of singlet oxygen formation for both ClAlPc loaded BSA NPs and free ClAlPc. Confocal microscopy confirmed the efficient cellular uptake and perinuclear localization of the ClAlPc loaded BSA NPs in HCT-116 cancer cells. In vitro cytotoxicity studies demonstrated a dose and time dependent photo-and sonotoxic effects in the presence of ClAlPc loaded BSA. In particular, simultaneous application of light and ultrasound as sono-photodynamic therapy (SPDT) resulted in 15 % cell viability in the presence of ClAlPc loaded BSA NPs, which is much lower than individual PDT and SDT results, confirming the effect of the combination therapy on cell viability. In comparison, free ClAlPc reduced cell viability to 27 %. These findings suggest that ClAlPc loaded BSA NPs is a promising "one-for-two" nanoplatform for combined cancer therapy to reduce the limitations of both methods.
  • Article
    A Simplified Molecular Imprinting Strategy Through Electrospinning of Polyacrylonitrile for Thin Film Microextraction of Selected Pesticides
    (Elsevier B.V., 2026) Şahin, A.; Akpinar, Y.; Yildirim, E.; Eroǧlu, A.E.; Boyaci, E.
    Molecularly imprinted polymers (MIPs) have been extensively used as selective extractive phases for sample preparation because of their analyte-selective binding sites. However, MIP preparation requires optimized monomer-template interactions and long polymerization reactions. In this study, a novel and simple method of MIP preparation was proposed based on electrospinning. Instead of preparing analyte-monomer complexes before polymerization, model analytes (trifluralin and carbaryl) were directly dissolved in a polyacrylonitrile (PAN) solution, then electrospun into nanofibrous mats. This allowed for a means of preparation of highly crystalline, template-imprinted nanostructures with minimal synthetic complexity. Following the characterization studies for the new material, the extraction properties of the imprinted and nonimprinted electrospun mats were investigated in thin film microextraction (TFME) studies by extracting trifluralin and carbaryl from water samples, followed by gas chromatography-mass spectrometry (GC–MS) analysis. The optimization results showed that samplers containing 10.0 mg of MIP sorbents made by electrospinning of a solution containing 5.0 mg of template in 1.0 mL of PAN solution resulted in approximately 4 and 7 times enhanced extraction recoveries for carbaryl and trifluralin compared to samplers made of non-imprinted bulk PAN. Moreover, the cross-reactivity testing performed with non-template analytes (malathion and diazinon) suggested a more specific extraction towards trifluralin compared to carbaryl. The proposed new technique was also validated using computational methodology, which supported the experimental finding about higher selectivity towards trifluralin. This may signify a probability for structural orientation of partially charged trifluralin under an electrical field in electrospun PAN creating favorable extraction sites. © 2025 Elsevier B.V.
  • Article
    Lipoxygenase Inhibitory Activity Evaluation of Achillea Biebersteinii Afan. by Activity-Guided Fractionation
    (Elsevier Ireland Ltd, 2026) Subasi, Bilgen; Gunbatan, Tugba; Gurbuz, Ihan; Dilmac, Elif; Bedir, Erdal; Demirci, Fatih
    Ethnopharmacological relevance: Achillea biebersteinii Afan. is traditionally utilized as folk medicine for a broad range of therapeutic applications, especially for promoting the maturation of abscesses, wound healing; against inflammation, and rheumatism in T & uuml;rkiye. Aim of the study: The anti-inflammatory potential of A. biebersteinii was evaluated through activity-guided fractionation (AGF) targeting lipoxygenase (15-LOX) inhibition. Materials and methods: Different chromatographic techniques were used for the AGF of the ethyl acetate extract of A. biebersteinii aerial parts. The in vitro 15-LOX inhibitory activity evaluation was performed to address the antiinflammatory activity. The structures of purified compounds from the fractions were confirmed by LC-HRMS, 1H NMR, and 13C NMR analyses, respectively. Results: The fractionation and isolation process culminated in the identification of three key flavonoids namely; patulitrin, axillarin, quercetagetin-7-O-beta-glucopyranoside, and 4,5-dicaffeoylquinic acid, which showed statistically remarkable 15-LOX inhibitory activity with inhibition rates of 82.27%, 96.81 %, 84.65% and 77.47 %, respectively. Two flavonoids were isolated by using the AGF method, where quinic acid was spotted to have significant 15-LOX inhibitory activity. Conclusion: These findings support the future therapeutic potential of A. biebersteinii as a natural antiinflammatory source.
  • Article
    Salt Tolerance Potential of Selected Solanum Pennellii Introgression Lines: Unique Shoot and Root Responses
    (Wiley, 2025) Yildiz, Hatice Selale; Doganlar, Sami; Frary, Anne
    Salinity stress affects agricultural lands worldwide, causing serious yield losses. Investigation of the salinity response and tolerance mechanisms of crop plants and their wild relatives is important for developing tolerant varieties. In this study, three Solanum pennellii introgression lines (IL2-5, IL7-4-1, IL8-3), reported to be tolerant to abiotic stress, were investigated for their physiological and molecular responses to severe salinity (200 mM NaCl). The findings emphasized the variety of different responses that even highly genetically similar lines can have to stress. In IL2-5, a lack of significant root and shoot growth reduction due to salinity was associated with the up-regulation of vacuolar ion transporter genes (NHX1 and NHX3) and the lowest Na+ and Cl- accumulation in leaves, while beneficial K+ levels were preserved. In IL7-4-1, lateral root development was exceptionally strong compared to the other lines, with high Na+ and Cl- accumulation in leaves due to this unique root architecture. Despite this, the negative effects were lower on IL7-4-1's shoot growth than in IL8-3 and the control cultivar M82 due to effective reactive oxygen species management and increased superoxide gene expression. IL8-3's growth response was most similar to M82; however, it was better able to maintain beneficial K+ levels under salt stress. Overall, it was revealed that S. pennellii has multiple salt tolerance mechanisms associated with specific chromosomal segments and unique plant architecture. In addition to contributing to a better understanding of the mechanisms of salinity tolerance, these findings provide important information for increasing tolerance through targeted breeding.
  • Article
    Citation - WoS: 8
    Protective and Therapeutic Effects of Milrinone on Acoustic Trauma in Rat Cochlea
    (Springer, 2019) Ceylan, Seyit Mehmet; Uysal, Erdal; Altinay, Serdar; Sezgin, Efe; Bilal, Nagihan; Petekkaya, Emine; Gulbagci, Mustafa Emre
    ObjectiveThe aim of this study was to investigate the potential protective and therapeutic effects of milrinone, a specific phosphodiesterase (PDE) III inhibitor, on acoustic trauma-induced cochlear injury and apoptosis.MethodsA total number of 30 healthy Wistar albino rats were evenly divided into five groups as follows: group 1 was assigned as control group; group 2 and 3 were assigned as low-dosage groups (0.25mg/kg) in which milrinone was administered 1h before acoustic trauma (AT) and 2h after AT, respectively; group 4 and 5 were assigned as high-dosage groups (0.50mg/kg) in which the drug was administered 1h before AT and 2h after AT, respectively. Except control group, all treatment groups received a single dosage of milrinone for 5days. Distortion product otoacoustic emissions (DPOAE) measurements were recorded before AT as well as at second and fifth post-traumatic days. At the end of fifth day, all rats were sacrificed and the cochlea of the rats was removed for histopathological evaluation. In addition, the groups were compared in terms of apoptotic index via caspase-3 staining.ResultsIn terms of signal-to-noise ratio (SNR), there was no statistically significant difference among the groups following AT (p>0.05). After 5days of milrinone treatment, the best SNR values were found in group 5, though all groups did not statistically differ (p>0.05). In histopathological evaluation, vacuolization, inflammation, and edema scores in all treatment groups were statistically lower than those of the control group (p<0.05). In group 2 and 4 where the drug was administered before AT, the inflammation and apoptosis index was lower than those of group 3 and 5 where the drug was administered after AT (p<0.0001).ConclusionWe reveal that milrinone has a protective effect on cochlear damage in the experimental acoustic model of rats. This protective effect was more apparent following the pre-traumatic milrinone administration, and is associated with its effect on decreasing inflammation and apoptosis. Based on DPOAE measurements following AT, especially in the group 5 (high-dosage group), milrinone may also have a therapeutic effect.
  • Article
    Citation - WoS: 8
    The Effects of Halofuginone on Wound Healing in the Rat Nasal Mucosa
    (Sage Publications Inc, 2020) Ceylan, Seyit Mehmet; Uysal, Erdal; Sokucu, Mehmet; Sezgin, Efe; Kanmaz, Mahmut Alper; Yurtseven, Duygu Gok; Bilal, Nagihan
    Background Halofuginone is an alkaloid febrifugine analogue and bioactive molecule that was isolated incidentally from the Dichroa febrifuga plant. The therapeutic efficacy of halofuginone in parasitic infections, scleroderma, inflammation, and fibrosis-related diseases, as well as in some types of cancer, has been previously reported. The effects of halofuginone on nasal mucosal damage are not yet known. Objective The aim of this study was to investigate the potential effect of topically applied halofuginone on wound healing in the mechanically injured nasal mucosa of rats. Methods A unilateral mucosal wound was created in the nasal cavity of 32 rats (aged 4 weeks) using the brushing technique. These rats were randomly divided into 4 groups. Although the control group did not receive an intervention, a dry pad, a saline-impregnated pad, or a pad impregnated with halofuginone were placed in the rats of the other 3 groups and left for 5 minutes. Rats were sacrificed on the 14th day, and a histological examination was performed. The nasal mucosa was assessed via hematoxylin-eosin and Masson's trichrome staining. Results There were no statistically significant differences in epithelial thickness, inflammation, goblet cell formation, and epithelial disarray values between the halofuginone group and the control group (P > .05). The subepithelial thickness was significantly decreased in the saline-treated group and the halofuginone-treated group (P < .05), but a significantly lower level of subepithelial fibrosis was only observed in the halofuginone group compared to the other groups (P < .05). Conclusions Topical halofuginone administration reduces the development of fibrosis and subepithelial edema after experimentally induced nasal mucosal injury, but it does not exert therapeutic or preventive effects on epithelial damage, inflammation, and goblet cell hyperplasia.
  • Article
    Chain-Length Dependent and Synergistic Prebiotic Effects of Xylooligosaccharides and Xylan on the Fecal Microbiota of Mice in Vitro
    (Elsevier, 2025) Sabanci, Kevser; Gulec, Sukru; Buyukkileci, Ali Oguz
    Oligomeric and polymeric prebiotics differ in their structural complexity, which influences microbial accessibility and fermentation kinetics. This study investigated the microbial responses to xylooligosaccharides (XOS), xylan (XY), and their combinations in comparison with inulin (INU) using an in vitro model inoculated with BALB/c mice fecal microbiota. Temporal analyses over 48 h included substrate consumption, acid production, and changes in microbial diversity. XOS was rapidly fermented, yielding high acetate and lactate levels, whereas XY was utilized more slowly due to its polymeric structure. During XY fermentation, xylobiose (X2) and xylotriose (X3) accumulated transiently, suggesting a stepwise depolymerization and utilization mechanism. The XOS + XY mix showed enhanced prebiotic effect, producing the highest amount of acid (151.8 mmol/L) and notably promoted the simultaneous enrichment of Bifidobacterium (12.5-fold), Bacteroides (8.85-fold), and Lactobacillus (14.9-fold) species compared to individual treatments These findings demonstrate that coadministered XOS and XY highlights the potential for designing tailored prebiotic formulations to optimize microbiota modulation, with potential relevance for human health.
  • Article
    Storage Protein Allergen Sensitization Patterns in Children: Insights from Multiplex Microarray Profiling and Hierarchical Clustering
    (Wiley, 2025) Caka, Canan; Ozcivici, Engin; Karakus, Ceyda Oksel; Sekerel, Bulent Enis
    Background Storage proteins (SPs), including 2S albumins, vicilins, and legumins, are key allergenic molecules (AMs) of peanuts, tree nuts (TNs), and sesame. Their structural stability contributes to allergenicity and sensitization. This study explored SP AM clustering patterns and evaluated the test performance of multiplex microarray (MM) testing in a pediatric cohort. Methods We retrospectively analyzed 350 children (median age: 3.7 years) with detectable SP sensitizations (>= 0.1 kU(A)/L) using the ALEX(2) MM platform. Sensitization interrelationships were analyzed using correlation heatmaps, hierarchical clustering (HC), dimensionality reduction, and feature elimination. Predictive utility was assessed through ROC curve analysis at different sensitization cut-offs (>0.1 and >0.3 kU(A)/L) and total IgE thresholds (>0, >20, and >50 kU/L). Results HC identified a broad SP cluster spanning peanuts, TNs, sesame, poppy seed, and buckwheat. Strong correlations and early HC linkages suggested extensive cross-sensitization (e.g., Ana o 3-Pis v 1 and Jug r 4-Cor a 9), alongside evidence of co-sensitization and molecular spreading. Unexpected clustering of structurally dissimilar peanut and pistachio AMs pointed to shared epitopes and/or cross-contamination. 2S albumins (Ara h 2, Cor a 14, Jug r 1, Ana o 3, and Ses i 1) were most predictive for clinical reactivity. Lower cut-offs and exclusion of patients with low total IgE improved test performance. Alpha-hairpinin (Pap s 2S albumin) showed potential as specific markers. Conclusions MM testing enables detailed SP sensitization profiling. Cluster-based interpretation may clarify cross- vs. co-sensitization, supporting informed clinical decisions. Use of recombinant AMs and IgE stratification may further enhance MM utility in food allergy diagnostics.