PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7645

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Author: search.filters.author.Yıldız, Ümit HakanPublisher: search.filters.publisher.John Wiley and Sons Inc.

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  • Article
    Citation - WoS: 6
    Citation - Scopus: 8
    Outer-Membrane Protease (ompt) Based E.coli Sensing With Anionic Polythiophene and Unlabeled Peptide Substrate
    (John Wiley and Sons Inc., 2020) Sinsinbar, Gaurav; Yıldız, Ümit Hakan; Gudlur, Sushanth; Wood, Sarah E.; Ammanath, Gopal; Yıldız, Ümit Hakan; Alagappan, Palaniappan; Liedberg, Bo; 04.01. Department of Chemistry; 04. Faculty of Science; 01. Izmir Institute of Technology
    E. coli and Salmonella are two of the most common bacterial pathogens involved in foodborne and waterborne related deaths. Hence, it is critical to develop rapid and sensitive detection strategies for near-outbreak applications. Reported is a simple and specific assay to detect as low as 1 CFU mL(-1)of E. coli in water within 6 hours by targeting the bacteria's surface protease activity. The assay relies on polythiophene acetic acid (PTAA) as an optical reporter and a short unlabeled peptide (LL37(FRRV)) previously optimized as a substrate for OmpT, an outer-membrane protease on E. coli. LL37(FRRV)interacts with PTAA to enhance its fluorescence while also inducing the formation of a helical PTAA-LL37(FRRV)construct, as confirmed by circular dichroism. However, in the presence of E. coli LL37(FRRV)is cleaved and can no longer affect the conformations and optical properties of PTAA. This ability to distinguish between an intact and cleaved peptide was investigated in detail using LL37(FRRV)sequence variants.
  • Article
    Citation - WoS: 56
    Citation - Scopus: 55
    A Bodipy-Based Fluorescent Probe To Visually Detect Phosgene: Toward the Development of a Handheld Phosgene Detector
    (John Wiley and Sons Inc., 2018) Sayar, Melike; Karakuş, Erman; Emrullahoğlu, Mustafa; Yıldız, Büşra; Yıldız, Ümit Hakan; Yıldız, Ümit Hakan; 04.01. Department of Chemistry; 04.04. Department of Photonics; 04. Faculty of Science; 01. Izmir Institute of Technology
    A boron-dipyrromethene (BODIPY)-based fluorescent probe with a phosgene-specific reactive motif shows remarkable selectivity toward phosgene, in the presence of which the nonfluorescent dye rapidly transforms into a new structure and induces a fluorescent response clearly observable to the naked eye under ultraviolet light. Given that dynamic, a prototypical handheld phosgene detector with a promising sensing capability that expedites the detection of gaseous phosgene without sophisticated instrumentation was developed. The proposed method using the handheld detector involves a rapid response period suitable for issuing early warnings during emergency situations.