PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7645

Browse

Filters

20252

Settings

Has files: NoInstitution Author: search.filters.institutionAuthor.Sofuoğlu, Aysun

Search Results

Now showing 1 - 2 of 2
  • Article
    Citation - WoS: 1
    Citation - Scopus: 1
    Airborne and Dust-Bound PBDEs Indoors and Outdoors in Izmir, Türkiye: A Multi-Route Exposure - Risk Assessment
    (Elsevier Sci Ltd, 2025) Genisoglu, Mesut; Edebali, Ozge; Sofuoglu, Aysun; Turgut, Cafer; Sofuoglu, Sait C.
    Phased-out flame retardants, e.g., polybrominated diphenyl ethers (PBDEs), persist in environmental media due to their resistance to degradation and ongoing emissions from PBDE containing materials and industrial activities. This study addresses a notable data gap in a unique setting, i.e., & Idot;zmir, T & uuml;rkiye, by investigating PBDE levels at homes, schools, and caf & eacute;/bar/restaurants, and assessing exposure and associated health risks. Indoor and outdoor air and dust samples were collected from rural, suburban, and urban areas. Exposure through ingestion, dermal absorption, and inhalation routes, and associated chronic-toxic and carcinogenic risks were estimated with Monte Carlo Simulation. Despite having been phase-out, house-dust Sigma BDE concentrations remained prevalent with average levels of >2000 ng/g in schools and homes, while outdoors they were <500 ng/g. BDE-209 was the predominant congener with an indoor air concentration of 486 pg/m(3) in schools and 56.7 pg/m(3) in homes. BDE-209 contributed 83.5-90.4 % of the indoor air Sigma BDE concentration in schools, while in homes this contribution ranged from 70.8 to 75.8 %. Aggregate exposure estimates show the predominant PBDE congener, BDE-209, was primarily exposed by accidental ingestion (58.6 %) followed by dermal absorption (21.9 %) and inhalation (19.5 %). Chronic-toxic risk (CTR, for BDE-47, BDE-99, BDE-153, and BDE-209) and carcinogenic risk (CR, for BDE-209) for the ingestion and dermal absorption routes indicated that house-dust and indoor-air PBDE exposures are not found to be considerable for human health. However, the contribution of inhalation route to the aggregate exposure of BDE-28, BDE-47, BDE-100, BDE-99 (87.0 %, 60.5 %, 54.3 %, and 57.3 %, respectively) may indicate the evermore PBDE exposure by inhalation for lower brominated congeners as they become more significant through environmental debromination of the predominant BDE-209.
  • Article
    Enhanced Catalytic Performance of Rhizomucor Miehei Lipase on Di-N and Diethylhexyl Phthalates: Insights Into Substrate Specificity and Immobilization Strategy
    (Taylor & Francis Ltd, 2025) Balci, Esin; Rosales, Emilio; Curras, Marta Pazos; Sofuoglu, Aysun; Sanroman, M. A.
    Di-n-butyl (DnBP) and Diethylhexyl Phthalates (DEHP), known as potential endocrine disruptors, are priority pollutants categorized by many regulatory agencies. Enzymatic degradation is a green and efficient approach to remove PEs in the environment. In this study, the DnBP and DEHP degradation performance of Rhizomucor miehei lipase (palatase) in free and immobilized forms on Halloysite nanoclays (HNCs) in an aqueous system was investigated. Upon enzyme immobilization, the alterations in the palatase's secondary structure were examined using the circular dichroism (CD) analysis. The binding affinity of DnBP and DEHP to palatase was evaluated with molecular docking approaches. The enzyme's immobilization efficiency and relative activity were found to be 80.3% and 87.8%, respectively. CD results revealed that palatase retained its secondary structure to a significant extent. HNCs-palatase (HNCs-P) exhibited a high stability, as the structural integrity of palatase was mostly preserved. Both free palatase (FP) and HNCs-P fully degraded DnBP and DEHP (100 mg/L) to phthalic acid and a degradation pathway of DnBP and DEHP was suggested. Immobilization prevented the enzyme inhibition caused by the accumulation of metabolites. After seven consecutive uses, HNCs-P was still able to degrade DnBP (63.3%) and DEHP (72.8%). Molecular docking results showed that DEHP had a higher affinity for palatase than DnBP. This study suggests that enzyme immobilization onto HNCs can increase their stability and catalytic performance. FP and HNCs-P effectively hydrolyse ester bonds responsible for phthalate toxicity. Considering their high efficiency, FP and HNCs-P can be used as potential phthalate degraders in various environmental remediation processes.