PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection

Permanent URI for this collectionhttps://hdl.handle.net/11147/7645

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Access type: Metadata onlyPublisher: search.filters.publisher.Royal Society of Chemistry

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  • Article
    Iron Oxide Nanocube Assembly on Silver Nanowire Templates to Enhance Magnetic Hyperthermia Performance
    (Royal Society of Chemistry, 2026) Arica, Tugce A.; Tiryaki, Ecem; Sadeghi, Ehsan; Mannir, Abubakar R.; Balci, Sinan; Pellegrino, Teresa
    Iron oxide nanocubes (IONCs) represent one of the benchmark magnetic nanoparticles able to most efficiently convert magnetic energy into heat for magnetic hyperthermia cancer treatment, and their heat losses can be further increased by controlling their assembly through the synthesis of ordered structures. However, achieving the alignment of nanoparticles with one-dimensional chain or columnar structures into long arrays to then study their magnetic heat losses still remains a significant challenge. This study exploits silver nanowires as high-surface-area anisotropic templates for the controlled chaining of IONCs. The surfaces of the IONCs were purposely functionalized with polyethyleneimine (IONCs@PEI) and interacted with silver nanowire (AgNW) surfaces via electrostatic attraction. Here, alternating current (AC) magnetometry was employed to compare the heating performance expressed as specific absorption rate values between individually coated IONCs@PEI and AgNWs@IONCs@PEI composites at various magnetic field strengths and frequencies. SAR values reveal that clustering of IONCs on AgNW surfaces improves the heating efficiency at an applied magnetic field strength of 24 kA m-1, regardless of the applied frequencies, with SAR values of AgNWs@IONCs@PEI composites outperforming those of individual IONCs@PEI. Moreover, dynamic hysteresis loops showed that the coercive field of AgNWs@IONCs@PEI increased significantly at 24 kA m-1, indicating the existence of strong magnetic dipolar interactions between nanoparticles. This study shows an innovative approach for guiding the orientation of magnetic nanoparticles using one-dimensional templates to enhance their heating performance.
  • Article
    Development of Self-Assembled Peptide Hydrogels Containing Matrix-Metalloproteinase Degradable Motifs for 3D Lung Cancer Models
    (Royal Society of Chemistry, 2026) Tarim, Burcu Sirma; Tamburaci, Sedef; Top, Ayben
    Hydrogel-forming peptides, including matrix metalloproteinase (MMP)-degradable motifs, have been employed to investigate cell-extracellular matrix interactions in vitro. However, their potential in 3D cancer models has been explored only in a few studies. In this study, we used modified MMP-2 degradable motifs (VSLRA or ASLRA) in the design of EDP1 (RVSLRADARVSLRADA) and EDP2 (RASLRADARASLRADA) peptide hydrogelators. The peptides self-assembled into nanofibrillar hydrogels with storage moduli between similar to 300 and similar to 400 Pa. MMP-2 degradation properties of the peptides were confirmed, and a slightly higher MMP-2 responsiveness of the EDP1 hydrogel was observed. The hydrogels were used in the encapsulation of A549 lung adenocarcinoma cancer cells and MRC-5 human lung fibroblast cells. The designed hydrogels supported the proliferation of these cells with high viability and induced cluster formation of encapsulated A549 cells similar to that observed with the RADA hydrogel. However, the hydrogel network structure affected the morphology of the migrated cells in the absence of curcumin. The addition of curcumin decreased the migration and invasion of A549 cells, resulting in a round cell morphology independent of the hydrogel matrices. Anticancer drug tests indicated that cell viability after drug treatment was higher in the 3D hydrogels than in 2D cultures. It was also confirmed that the combinational therapy of doxorubicin and curcumin decreased the cell proliferation and colonization to a greater extent compared to doxorubicin monotherapy. Thus, the hydrogels developed in this study can be used for 3D cancer models or other tissue engineering applications as an alternative to the RADA hydrogel by exploiting the MMP-2 degradation properties.
  • Article
    Citation - WoS: 16
    Citation - Scopus: 20
    Smartphone-Assisted Hepatitis C Detection Assay Based on Magnetic Levitation
    (Royal Society of Chemistry, 2020) Özefe, Fatih; Yıldız, Ahu Arslan
    This work describes development of smartphone-assisted magnetic levitation assay forPoint-of-Care(PoC) applications. Magnetic levitation is a technique that detects and separates particles based on their density differences in a magnetic field. Observation of the levitated micro-particles is mainly performed by light microscope or additional optical components, which mostly limits applicability of the magnetic levitation technique for PoC diagnostics. In this paper, we demonstrated the capability of the smartphone assisted-magnetic levitation platform for Hepatitis C (HCV) detection assay. This method utilizes microsensor beads (MS beads) that are functionalized with anti-HCV NS3 antibody. First, the magnetic levitation platform was optimizedviadensity marker polyethylene beads (DMB); then HCV NS3 protein was successfully detected based on levitation height differences of MS beads caused by density changes. The capability of the magnetic levitation platform for HCV detection was determined as almost 10-fold sensitive compared to conventional techniques such as enzyme-linked immunosorbent assay (ELISA). The imaging capability and resolution of the setup was improved over previously used configurations, and the developed platform enabled visualization of micro-scale objects only by smartphone assistance. This method requires no power, it is an easy-to-use and cost effective, therefore it could be easily adaptable to varied sensing assays as PoC tool.