PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7645
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Article Epigallocatechin Gallate and Punicalagin Combination Reduces Aβ Aggregation and Promotes Neurogenesis in Adult Zebrafish Brain(John Wiley and Sons Inc, 2026) Nazli, D.; Ipekgil, D.; Poyraz, Y.K.; Can, K.; Okmen, I.; Turhanlar-Sahin, E.; Ozhan, G.Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline, memory impairment, and behavioral alterations. The pathogenesis of AD involves the accumulation of amyloid-beta (Aβ) plaques and the hyperphosphorylated tau proteins, which disrupt neuronal function and trigger neuroinflammation. This study explores the therapeutic potential of epigallocatechin gallate (EGCG) and punicalagin (PU) in mitigating Aβ-induced toxicity using an adult zebrafish model of AD. Our results demonstrate that the EGCG + PU combination significantly reduces Aβ accumulation, protects against cellular damage, suppresses acetylcholinesterase (AChE) activity, and normalizes the expression of amyloidogenic and AD-related genes. Additionally, EGCG + PU treatment alleviates neuroinflammation by suppressing glial activation, including reductions in L-plastin and proinflammatory cytokine expression, while promoting neuronal recovery through mechanisms of neurogenesis and neuroprotection. Notably, the combination treatment restored neuronal density and improved behavioral outcomes by alleviating anxiety- and aggression-like behaviors associated with Aβ toxicity. These results underscore the synergistic neuroprotective effects of EGCG + PU, highlighting their potential as a novel therapeutic approach for mitigating the pathological, behavioral, and inflammatory aspects of AD. © 2026 Wiley Periodicals LLC.Article Valorization of Recycled Waste in Green/White Purification and LC-QTOF/MS Analysis of Beverages Adulterated with Incapacitating Drugs(Elsevier B.V., 2026) Anilanmert, Beril; Yonar, Fatma Cavus; Er, Elif Ozturk; Pekcaliskan, Elif Yılmaz; Cengiz, SalihIncapacitating drugs constitute a growing threat for the community, since victims may drink adulterated beverages without noticing. A validated eco-friendly/economical purification/analysis kit prototype, along with an LC-QToF/MS method has been developed in coke and mixed fruit-juice, for simultaneous determination of 10 drugs used for incapacitating victims (zaleplone, zolpidem, zopiclone, mephedrone, fentanyl, phenytoin, thiopental, sertraline, ketamine and GHB). A combination of two different waste nut-shells which yielded the highest recovery for these drugs were directly used as adsorbent after grinding and modification and a reusable separation apparatus recycled from waste were utilized for the first time in a toxicological analysis. In the method, after adding the adsorbent on to the sample, pH was adjusted. Following 25-min (min) automatic vortexing for adsorption, matrix was removed easily, using the separation apparatus. After 25-min desorption via cold ultrasonication using 500 μL methanol, a 9.5-min LC-QToF/MS analysis was performed. The validated method in fruit-juice and coke, extraordinarily gave successful results also in urine and saliva. Assessment tools for greenness/whiteness and pictograms confirmed the environmental friendliness of the method kit. © 2025 Elsevier B.V.Article Relationships Between Light Exposure and Aspects of Cognitive Function in Everyday Life(Springer Nature, 2025) Didikoglu, Altug; Woelders, Tom; Bickerstaff, Lucien; Mohammadian, Navid; Johnson, Sheena; van Tongeren, Martie; Lucas, Robert J.Light exposure can modulate cognitive function, yet its effects outside of controlled laboratory settings remain insufficiently explored. To examine the relationship between real-world light exposure and cognitive performance, we assessed personal light exposure and measured subjective sleepiness, vigilance, working memory, and visual search performance over 7 days of daily life, in a convenience sample of UK adults (n = 58) without significant circadian challenge (shiftwork or jet-lag). A subset of participants (n = 41) attended an in-lab session comprising a battery of pupillometric and psychophysical tests aimed to quantify melanopsin-driven visual responses. We find significant associations between recent light exposure and subjective sleepiness. Recent light exposure was also associated with reaction times for both psychomotor vigilance and working memory tasks. In addition, higher daytime light exposure and an exposure pattern with reduced fragmentation were linked to improved cognitive performance across visual search, psychomotor vigilance, and working memory tasks. Higher daytime light exposure and earlier estimated bedtimes were associated with stronger relationships between recent light exposure and subjective sleepiness. These results provide real world support for the notion that intra- and inter-individual differences in light exposure meaningfully influence aspects of cognition, with beneficial effects of short-term bright light and of habitual light exposure patterns characterized by brighter daytimes, earlier rest phase, and greater intra- and inter-daily stability.Article Interaction of Hazelnut-Derived Polyphenols With Biodegradable Film Matrix: Structural, Barrier, and Functional Properties(Multidisciplinary Digital Publishing Institute (MDPI), 2026) Hızır-Kadı, I.; Demircan, E.; Özçelik, B.The study presents a sustainable approach to valorizing hazelnut processing by-products, specifically skins and shells, through their conversion into bioactive polyphenol-rich extracts using pressurized hot water extraction (PHWE), an environmentally friendly green technology. PHWE yielded extracts with total phenolic contents of 25.4 mg GAE/g dw (shell) and 83.7 mg GAE/g dw (skin), which were incorporated into biodegradable poly(vinyl alcohol)/carboxymethyl cellulose (PVA/CMC) films at concentrations of 1–3% (w/v). The resulting composites were comprehensively characterized in terms of structural, mechanical, thermal, and barrier properties. FTIR, DSC, and XRD analyses demonstrated strong hydrogen bonding, increased thermal stability, and reduced crystallinity due to polyphenol–polymer interactions. Phenolic incorporation enhanced UV-blocking capability, increased antioxidant activity by up to five-fold, and reduced oxygen permeability from 0.048 to 0.015 (cm3·mm·m−2·day−1·atm−1) (69% reduction, p < 0.05), compared to neat PVA while maintaining desirable transparency (>70%). Optimal formulations (HSkE-II) exhibited a 39% increase in elongation at break and improved flexibility without compromising film integrity. Application tests using fresh-cut apples, watermelon, and chicken revealed significant reductions in microbial growth (up to ~1.2 log CFU/g), lipid oxidation, and weight loss during storage, confirming the films’ potential for active food packaging. This work highlights an efficient valorization strategy for nut industry by-products and demonstrates their functional integration into sustainable biodegradable packaging systems. © 2025 by the authors.Article A Simplified Molecular Imprinting Strategy Through Electrospinning of Polyacrylonitrile for Thin Film Microextraction of Selected Pesticides(Elsevier B.V., 2026) Şahin, A.; Akpinar, Y.; Yildirim, E.; Eroǧlu, A.E.; Boyaci, E.Molecularly imprinted polymers (MIPs) have been extensively used as selective extractive phases for sample preparation because of their analyte-selective binding sites. However, MIP preparation requires optimized monomer-template interactions and long polymerization reactions. In this study, a novel and simple method of MIP preparation was proposed based on electrospinning. Instead of preparing analyte-monomer complexes before polymerization, model analytes (trifluralin and carbaryl) were directly dissolved in a polyacrylonitrile (PAN) solution, then electrospun into nanofibrous mats. This allowed for a means of preparation of highly crystalline, template-imprinted nanostructures with minimal synthetic complexity. Following the characterization studies for the new material, the extraction properties of the imprinted and nonimprinted electrospun mats were investigated in thin film microextraction (TFME) studies by extracting trifluralin and carbaryl from water samples, followed by gas chromatography-mass spectrometry (GC–MS) analysis. The optimization results showed that samplers containing 10.0 mg of MIP sorbents made by electrospinning of a solution containing 5.0 mg of template in 1.0 mL of PAN solution resulted in approximately 4 and 7 times enhanced extraction recoveries for carbaryl and trifluralin compared to samplers made of non-imprinted bulk PAN. Moreover, the cross-reactivity testing performed with non-template analytes (malathion and diazinon) suggested a more specific extraction towards trifluralin compared to carbaryl. The proposed new technique was also validated using computational methodology, which supported the experimental finding about higher selectivity towards trifluralin. This may signify a probability for structural orientation of partially charged trifluralin under an electrical field in electrospun PAN creating favorable extraction sites. © 2025 Elsevier B.V.Article Efficacy of Fermented Foods for the Prevention and Treatment of Bacterial Vaginosis and Vulvovaginal Candidiasis(Frontiers Media SA, 2025) Akpinar, A.; Markiewicz, L.H.; Harsa, H.S.; Paveljšek, D.; Domínguez-Soberanes, J.; Agirbasli, Z.; Karakaş-Budak, B.Vaginal function in healthy women is closely associated with a lactobacilli-dominated microbiome. Among the most common conditions arising from dysbiosis are bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC). While the efficacy of oral probiotics for the treatment of BV and VVC is well documented, the role of consuming fermented foods remains underexplored. This systematic review aims to present a systematic evaluation of the potential role of fermented foods in the prevention and treatment of BV and VVC and establish the extant research gap between the realm of the clinical sciences and the field of food science and technology. For this purpose, under the guidance of COST Action CA20128—Promoting Innovation of Fermented Foods (PIMENTO), a systematic literature review was conducted in two phases. PubMed, Scopus, and Cochrane databases were used for Phase I to analyze articles on human trials and observational studies where the intervention/exposure involved oral consumption of fermented food. In Phase II, a two-step search strategy was employed: (i) identifying microorganisms with demonstrated clinical efficacy in managing BV and VVC, and (ii) reviewing food science literature where these strains are utilized for fermentation. It was observed that 87% of the food starter applications exploited only two of the 54 efficacious strains identified through clinical studies, namely Lactobacillus rhamnosus GG and Lactobacillus acidophilus LA-5. Findings underscore the potential of fermented foods as carriers for beneficial microorganisms and their relevance in supporting vaginal health. This review contributes to a deeper understanding of the interplay between nutritional consumption of viable probiotic strains and their importance in immunomodulation, highlighting the need for more integrated research efforts across disciplines. Future research aimed at filling this gap will enable informed clinical decisions and dietary recommendations. © © 2025 Akpınar, Markiewicz, Harsa, Paveljšek, Domínguez-Soberanes, Agirbasli, Naziri, El Jalil, Bouchaud, Salminen, Savary-Auzeloux, Humblot, Chassard, Pracer, Vergères and Karakaş-Budak.Article A Machine Learning Model for Predicting Oligoclonal Band Positivity Using Routine Cerebrospinal Fluid and Serum Biochemical Markers(Oxford University Press Inc, 2025) Gözgöz, Hazar; Orhan, Oğuzhan; Akan Konuk, Başak; Akan, PınarOBJECTIVE: To develop and validate a machine learning model for predicting oligoclonal band (OCB) positivity using routine cerebrospinal fluid (CSF) and serum biochemical markers to improve the diagnostic accuracy and efficiency of assessing intrathecal immunoglobulin G (IgG) synthesis. METHODS: In this retrospective study (n = 1709), an ensemble model was developed using 8 refined CSF and serum parameters. Combining optimized CatBoost, XGBoost, and LightGBM classifiers, the model was trained and evaluated using a 2-phase workflow, including 5-fold cross-validation and validation on independent internal (n = 342) and external (n = 49) cohorts. RESULTS: The developed ensemble model achieved a receiver operating characteristic-area under the curve (ROC-AUC) of 0.902 on the internal test set, significantly outperforming the conventional IgG index (ROC-AUC, 0.795). At its optimal threshold, the model demonstrated an accuracy of 0.830, with a sensitivity of 0.714 and a specificity of 0.916. On the external validation cohort, it achieved 90% accuracy and 96% sensitivity. CONCLUSIONS: A novel machine learning ensemble model accurately predicts OCB positivity using routine laboratory data and demonstrates superior performance compared with the IgG index. This approach represents a significant step in applying artificial intelligence in laboratory medicine, with the potential to enhance diagnostic efficiency. Prospective, multicenter validation is essential for broader clinical implementation. © The Author(s) 2025.Article Chemosensitizing Effect of Apigenin on T-ALL Cell Therapy(Frontiers Media SA, 2025) Huseynova, N.; Baran, Z.; Khalilov, R.; Mammadova, A.; Baran, Y.T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy with limited therapeutic options and frequent treatment-associated toxicities. L-asparaginase, a cornerstone in T-ALL therapy, is often restricted by hypersensitivity reactions and systemic side effects, highlighting the need for safer strategies to enhance its efficacy. This study investigated the potential of apigenin, a naturally occurring flavonoid with antioxidant and pro-apoptotic properties, to act as a chemosensitizer for L-asparaginase in MOLT-4 T-ALL cells. Cytotoxicity was assessed using the MTT assay, apoptosis by Annexin V/PI staining, cell cycle distribution by flow cytometry, and mitochondrial membrane potential by JC-1 staining. Both apigenin and L-asparaginase produced dose- and time-dependent cytotoxicity, with combination treatment resulting in reduced IC<inf>50</inf> values. Apoptotic analysis showed significantly higher apoptosis in the combination-treated groups than in single-agent groups. Cell cycle analysis revealed that apigenin induced S-phase arrest and L-asparaginase induced G1-phase arrest, while the combination disrupted cell cycle progression at multiple checkpoints. JC-1 assay further demonstrated enhanced mitochondrial depolarization, with up to a 29.2-fold increase in cytoplasmic-to-mitochondrial fluorescence ratio in combination therapy compared to L-asparaginase alone. These findings indicate that apigenin potentiates L-asparaginase-induced cytotoxicity through mitochondrial dysfunction and intrinsic apoptotic signaling. The combined use of apigenin and L-asparaginase may provide a novel strategy to improve therapeutic efficacy in T-ALL while potentially reducing the toxicity associated with high-dose L-asparaginase monotherapy. © © 2025 Huseynova, Baran, Khalilov, Mammadova and Baran.Article Impact of Yogurt Consumption on Bone Health Markers in Adults with or without Osteoporosis: A Systematic Review and Meta-Analysis(Frontiers Media SA, 2025) Mayo, B.; Malagón-Rojas, J.; Agahi, F.; Frias, J.; Künili, İ.E.; Kütt, M.-L.; Tamang, J.P.Yogurt is a fermented dairy product widely recognized for its nutritional value and potential health benefits, particularly due to its high content of calcium, phosphorus, vitamins, and other bioactive compounds. Its purported role in promoting bone health has attracted increasing attention, especially among adults at risk of osteoporosis. However, the specific impact of yogurt consumption on bone health remains controversial, as current evidence is limited and often inconclusive. This systematic review and meta-analysis sought to evaluate the exclusive effects of yogurt consumption on bone health in adults with and without osteoporosis. Employing rigorous inclusion and exclusion criteria and standardized methodologies, the study reviewed data from both observational and clinical studies to assess the effect of yogurt consumption on bone mineral density (BMD), fracture risk, and other bone health markers. In addition, yogurt characteristics were documented in accordance with EFSA guidelines, the possible bioactive components listed, and their potential mechanism of action in relation to bone health discussed. Studies fitting the inclusion and exclusion criteria (n = 12) were all observational, limiting the ability to infer causality. The meta-analysis of cohort studies (n = 6) found no significant association between yogurt consumption and hip fracture risk (HR = 1.01, 95% CI: 0.96–1.07), while the observed positive effect on BMD was minimal and clinically irrelevant (SMD = 0.009). Although some studies reported modest improvements in BMD and bone biomarkers, the overall evidence remains inconclusive, hindered by the heterogeneity in study designs and inconsistent yogurt intake. In conclusion, current evidence does not support a significant role of yogurt consumption in preventing fractures or improving BMD in adults. Well-designed randomized controlled studies are needed to clarify its effects, particularly in adults at risk of or with osteoporosis or osteopenia. In conclusion, the effectiveness of yogurt as a primary strategy for enhancing bone health is not fully substantiated by current data, though its inclusion within a balanced diet may still offer benefits for bone health. Systematic review registration: https://osf.io/, 10.17605/OSF.IO/ES2PM © 2025 Elsevier B.V., All rights reserved.Conference Object Citation - WoS: 24Effect of pH and Hydration on the Normal and Lateral Interaction Forces Between Alumina Surfaces(2006) Polat, Mehmet; Sato, Kimiyasu; Nagaoka, Takaaki; Watari, KojiNormal and lateral interaction forces between alumina surfaces were measured using Atomic Force Microscopy-Colloid Probe Method at different pH. The normal force curves exhibit a well-defined repulsive barrier and an attractive minimum at acidic pH and the DLVO theory shows excellent agreement with the data. The normal forces are always repulsive at basic pH and the theory fails to represent the measurements. Lateral forces are almost an order of magnitude smaller in the basic solutions. These differences, which have important implications in the study of stability and rheology, are attributed to the hydration of the alumina surface at basic pH. © 2013 Elsevier B.V., All rights reserved.