Food Engineering / Gıda Mühendisliği

Permanent URI for this collectionhttps://hdl.handle.net/11147/12

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Author: search.filters.author.Atabay, Halil İbrahimSubject: search.filters.subject.Arcobacter

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Now showing 1 - 3 of 3
  • Article
    Citation - WoS: 21
    Citation - Scopus: 24
    Isolation and Identification of Arcobacter Species From Environmental and Drinking Water Samples
    (Springer Verlag, 2016) Talay, Funda; Molva, Çelenk; Atabay, Halil İbrahim
    Water plays an important role in the transmission of Arcobacter spp. to animals and humans. The aim of this study was to isolate and characterize Arcobacter spp. from 115 different water samples (66 sewage, 25 rivers, 16 spring water, and 8 drinking water) in Izmir, Turkey. In total, 41 samples (35.7 %) were found positive for Arcobacter spp. by the genus-specific PCR. Arcobacter butzleri was detected in 39 out of 115 samples (33.9 %) including 24 sewage, 13 rivers, and 2 spring water. The remaining Arcobacter spp. (n = 2) isolates could not be identified by m-PCR and 16S rRNA gene sequencing. Based on the phenotypic characterization, most of the Arcobacter species (87.8 %) indicated weak catalase activity. In addition, there were differences in phenotypic patterns among isolated species during growth at 37 °C under microaerobic and aerobic conditions, in the presence of 2 % (39/41) and 3.5 % (32/41) NaCl and 0.04 % TTC (39/41) and on MacConkey agar (38/41). The results of this study indicated that environmental water samples are common sources for Arcobacter spp. Therefore, effective control measures should be taken to protect human health.
  • Article
    Citation - WoS: 27
    Citation - Scopus: 28
    Isolation of Various Arcobacter Species From Domestic Geese (anser Anser)
    (Elsevier Ltd., 2008) Atabay, Halil İbrahim; Ünver, Ahmet; Şahin, Mitat; Otlu, Salih; Elmalı, Mehmet; Yaman, Hilmi
    In this study, the prevalence and distribution of various Arcobacter spp. were investigated in samples taken from the cloacae of healthy domestic geese raised in Turkey. A membrane filtration technique with a non-selective blood agar was employed after enrichment in Arcobacter enrichment broth (AEB) to isolate a wide range of Arcobacter spp. In addition, the isolates were characterized phenotypically and identified at species level using a multiplex-PCR assay. A total of 90 cloacal swab samples taken from geese, collected on three farms (18, 25, 47 samples, respectively), were examined. Of the samples examined, 16 (18%) were found positive for Arcobacter. One Arcobacter species was isolated from each bird. Of the 16 Arcobacter isolates, 7 (44%), 7 (44%) and 2 (12.5%) were identified by m-PCR as A. cryaerophilus, A. skirrowii and A. butzleri, respectively. The present study indicates that domestic geese can harbour a variety of Arcobacter spp. in their cloacae. The presence of Arcobacter in geese may be of significance as reservoirs in their dissemination. Detailed research is needed for better understanding of the epidemiology and zoonotic potential of this emerging pathogen.
  • Article
    Citation - WoS: 2
    Citation - Scopus: 4
    Genotyping of Various Arcobacter Species Isolated From Domestic Geese by Randomly Amplified Polymorphic Dna (rapd) Analysis
    (M. & H. Schaper, 2008) Atabay, Halil İbrahim; Ünver, Ahmet; Otlu, Salih; Kalaycıoğlu, Atila Taner
    The present study was undertaken to genotype Arcobacter (A.) butzleri, A. cryaerophilus and A. skirrowii isolates from domestic geese from three different flocks in Turkey. Fifteen Arcobacter isolates were analysed to determine the RAPD profiles based on the amplified DNA fragment patterns using a universal primer for genotyping. 7 A. cryaerophilus, 2 A. butzleri and 6 A. skirrowii isolates produced 6, 2 and 3 distinct profiles, respectively. The isolates of the same patterns originated from the same flocks. The findings of the present study may support previous reports of the existence of a large degree of heterogeneity among Arcobacter isolates. Observation of such levels of genetic diversity may suggest that there are multiple contamination sources in the environment and/or the determined genotypes may have undergone genetic rearrangements. This first report of genotyping of various Arcobacter species isolated from healthy geese is expected to improve the understanding of the ecology and epidemiology of this emerging pathogen.