Food Engineering / Gıda Mühendisliği

Permanent URI for this collectionhttps://hdl.handle.net/11147/12

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Author: search.filters.author.Molva, ÇelenkScopus Q: search.filters.scopusQuartile.Q3

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  • Article
    Citation - WoS: 12
    Citation - Scopus: 14
    Prevalence, Virulence Characterization, and Genetic Relatedness of Listeria Monocytogenes Isolated From Chicken Retail Points and Poultry Slaughterhouses in Turkey
    (Springer, 2019) Çoban, Ayşen; Pennone, Vincenzo; Sudağıdan, Mert; Molva, Çelenk; Jordan, Kieran; Aydın, Ali
    Listeria monocytogenes is one of the most important foodborne pathogens and is a causal agent of listeriosis in humans and animals. The aim of this study was to determine the prevalence, serogroups, antibiotic susceptibility, virulence factor genes, and genetic relatedness of L. monocytogenes strains isolated from 500 poultry samples in Turkey. The isolation sources of 103 L. monocytogenes strains were retail markets (n = 100) and slaughterhouses (n = 3). L. monocytogenes strains were identified as serogroups 1/2a-3a (75.7%, lineage I), 1/2c-3c (14.56%, lineage I), 1/2b-3b-7 (5.82%, lineage II), 4a-4c (2.91%, lineage III), and 4b-4d-4e (0.97%, lineage III). Most of the L. monocytogenes strains (93.2%) were susceptible to the antibiotics tested. PCR analysis indicated that the majority of the strains (95% to 100%) contained most of the virulence genes (hylA, plcA, plcB, prfA, mpl, actA, dltA, fri, flaA inlA, inlC, and inlJ). Pulsed-field gel electrophoresis (PFGE) demonstrated that there were 18 pulsotypes grouped at a similarity of >90% among the strains. These results indicate that it is necessary to prevent the presence of L. monocytogenes in the poultry-processing environments to help prevent outbreaks of listeriosis and protect public health.
  • Article
    Citation - WoS: 5
    Modeling Growth of Alicyclobacillus Acidoterrestris Dsm 3922 Type Strain Vegetative Cells in the Apple Juice With Nisin and Lysozyme
    (AIMS Press, 2017) Molva, Çelenk; Baysal, Ayşe Handan
    In the present study, the effect of storage temperature on A. acidoterrestris DSM 3922 cells (105 CFU/mL) was examined during growth in reconstituted apple juice (pH 3.8, degrees Brix 11.3) containing nisin (0-100 IU/mL) and lysozyme (0-100 mg/L). The growth curves were obtained at three temperatures of 27, 35 and 43 degrees C using absorbance data (OD600nm). Based on the results, the minimal inhibitory concentrations (MICs) of nisin were found as 10 IU/mL at all tested temperatures. On the other hand, increasing the temperature decreased the amount of lysozyme for growth inhibition. The MICs of lysozyme were found as 10, 2.5 and 1.25 mg/L at 27, 35 and 43 degrees C, respectively. At selected non-inhibitory doses, nisin (1.25-5 IU/mL) and lysozyme (0.3-2.5 mg/L) prolonged the lag time compared to the controls at the corresponding temperatures. In addition, there was a strong linear relationship between the lag time and lysozyme concentrations at 27 and 35 degrees C (R-2 > 0.98). The results of this study demonstrated that both nisin and lysozyme could be used to inhibit the growth of A. acidoterrestris cells in the apple juice. The results also indicated that the growth parameters were variable depending on the storage temperature and the type of the antimicrobial agent used in the apple juice.
  • Article
    Citation - Scopus: 7
    Human Acute Gastroenteritis Associated With Arcobacter Butzleri
    (Elsevier Ltd., 2012) Kayman, Tuba; Atabay, Halil İbrahim; Abay, Seçil; Hızlısoy, Harun; Molva, Çelenk; Aydın, Fuat
    Arcobacter spp. are considered emerging food-borne pathogens ( 1). Contaminated water and meat play an important role in the transmission of these bacteria to humans ( 2 ; 3). Currently, the genus Arcobacter has 13 recognized species: A. butzleri, A. cryaerophilus, A. skirrowii, A. nitrofigilis, A. cibarius, A. halophilus, A. mytili, A. thereius, A. marinus, A. trophiarum, A. defluvii, A. molluscorum, and A. ellisii ( 4 ; 5). Only three species, namely, A. butzleri, A. cryaerophilus, and A. skirrowii, have been associated with human and animal diseases ( 1). These microorganisms cause a number of infections, such as abortion, mastitis, and septicemia, in animals. In humans, they have been reported to cause gastrointestinal tract infections and septicemia ( 6 ; 7). Arcobacter septicemia secondary to underlying diseases, such as cirrhosis ( 8) and acute gangrenous appendicitis (9), has also been documented in humans.