Food Engineering / Gıda Mühendisliği

Permanent URI for this collectionhttps://hdl.handle.net/11147/12

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Author: search.filters.author.Severcan, Feride

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  • Article
    Citation - WoS: 4
    Citation - Scopus: 5
    Biophysical and Microbiological Study of High Hydrostatic Pressure Inactivation of Bovine Viral Diarrheavirus Type 1 on Serum
    (Elsevier Ltd., 2012) Ceylan, Çağatay; Severcan, Feride; Özkul, Aykut; Severcan, Mete; Bozoğlu, Faruk; Taheri, Nusret
    The effect of high hydrostatic pressure application on fetal bovine serum components and the model microorganism (Bovine Viral Diarrheavirus type 1 NADL strain) was studied at 132 and 220MPa pressure for 5min at 25°C. Protein secondary structures were found to be unaffected by an artificial neural network application on the amide I region for both untreated and HHP treated samples. FTIR spectroscopy study of both the HHP-treated and control samples revealed changes in the intensity of some bands in the finger-print region (1500-900cm -1) originating mainly from lipids which are thought to result from changes in the lipoprotein structure. The virus strain lost its infectivity completely after 220MPa HHP treatments. These results indicate that HHP can be successfully used for inactivation of pestiviruses while leaving structural and functional properties of serum and serum products unaffected. © 2011 Elsevier B.V.
  • Article
    Citation - WoS: 3
    Citation - Scopus: 3
    Evaluation of High Hydrostatic Pressure Effects on Bovine Red Blood Cells and Platelets
    (Taylor and Francis Ltd., 2009) Ceylan, Çağatay; Severcan, Mete; Bozoğlu, Faruk; Severcan, Feride
    The objective of this study was to investigate the effects of high hydrostatic pressure (HHP) on the stability of red blood cells (RBCs) and platelets. Bovine blood cells (n=5) were treated with the pressure of 55, 110, 154 and 220MPa at 25°C for 5min. Light microscopy, atomic force microscopy and flow cytometry studies revealed that RBCs were morphologically stable up until the 220MPa pressure treatments, at which surface modifications were observed. The platelets were found to be less stable than RBCs. HHP application did not cause any significant change in the signal intensity, band area and frequency values of the infrared bands with the exception that a significant variation was observed in the area of the cholesterol band. No statistically significant variations were observed in the secondary structure elements due to HHP treatment according to the artificial neural network study based on the FTIR data.