Food Engineering / Gıda Mühendisliği
Permanent URI for this collectionhttps://hdl.handle.net/11147/12
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Article Citation - WoS: 8Citation - Scopus: 9Unrevealing the Impact of Pulsed Electric Fields (pef) on Cucumber Seed Vigour and Surface Disinfection(Sciendo, 2021) Atmaca, Bahar; Akdemir Evrendilek, Gülsün; Bulut, Nurullah; Uzuner, SibelChemicals used for seed treatments help to increase the agricultural production by preventing pests and pathogens but also cause environmental and health problems. Thus, environmentally-friendly technologies need to be developed for a seed treatment that inactivates surface microflora and improves seed vigor. One such pulsed electric field (PEF) treatment applied to cucumber seeds in the range of 1.07-17.28 Joule (J) significantly enhanced a mean germination rate (MGR) by up to 9%, a normal seedling rate by 25.73%, and a resistance to 100 and 200 mM salt stresses by 96% and 91.67%, respectively, with a stronger and faster growth of roots and seedlings. PEF treatment provided 3.34 and 3.22 log-reductions in the surface microflora of total mold and yeast and total aerobic mesophilic bacteria, respectively. The electrical conductivity (EC) values of the control samples increased over time, from 4 to 24 h. Those of the PEF-treated samples after 4, 12, and 24th hours were also more affected by the measurement time not by the PEF treatment. The joint optimization of 18 responses based on the best-fit Gaussian process model pointed to 19.78 s and 17.28 J as the optimal settings. The PEF treatment appeared to improve seed germination ability and stress resistance with the adequate inactivation of surface microflora.Article Citation - WoS: 52Citation - Scopus: 69Identification of Equivalent Processing Conditions for Pasteurization of Strawberry Juice by High Pressure, Ultrasound, and Pulsed Electric Fields Processing(Elsevier, 2019) Yıldız, Semanur; Pokhrel, Prashant Raj; Ünlütürk, Sevcan; Barbosa-Canovas, Gustavo, VThe objective of this study was to evaluate the effectiveness of high pressure processing (HPP), ultrasound (US) and pulsed electric fields (PEF) for the pasteurization of strawberry juice (SJ). Acid-adapted Escherichia coli was used to inoculate SJ prior to treatment with HPP, US, and PEF. HPP was applied at several pressures (200-400 MPa) up to 2 min while US (120 mu m, 24 kHz) was conducted at 25, 40, and 55 degrees C up to 10 min in continuous pulsing mode. In order to avoid excessive use of SJ, PEF was performed using a model solution (MS) basically composed of citric acid (8 g/L), fructose (35 g/L), glucose (35 g/L), Na2HPO4 (0.2 M) and NaCl (5%) to simulate the SJ electrical conductivity, pH, and total soluble solid (TSS). A face-centered composite design was conducted for PEF processing at different electric field intensities (EFI) (25-35 kV/cm) and treatment times (5-27 mu s). Processing conditions were selected that resulted in 5-log CFU/mL inactivation of E. coli. HPP at 300 MPa for 1 min, and US at 55 degrees C (thermosonication) for 3 min reduced E. coli in SJ by 5.75 +/- 0.52 and 5.69 +/- 0.61 log CFU/mL, respectively. PEF treatment at 35 kV/cm, 27 mu s treatment time, 350 mL/min flow rate, and 2 mu s pulse width in monopolar mode resulted in 5.53 +/- 0.00 log reduction of E. coli in MS. Likewise, E. coli population in SJ was also reduced by 5.16 +/- 0.15 log after applying the same PEF conditions to SJ. No E. coli was detected in SJ subjected to conventional thermal pasteurization at 72 degrees C for 15 s. All technologies reduced the natural microbiota below 2 log CFU/mL in terms of the total aerobic bacteria and yeast-mold counts. Thus, this study identified the equivalent conditions for the SJ pasteurization by three nonthermal processing technologies. Industrial relevance: Consumers have an increasing interest towards fresh-like food products with desirable nutritional and sensorial attributes. High pressure, ultrasound and pulsed electric field are three relevant novel nonthermal technologies as alternatives to conventional thermal treatments. This study identified the processing conditions of these three nonthermal technologies for the pasteurization of strawberry juice based on equivalent inactivation of acid-adapted E. coli. From an industrial point of view, the established processing conditions are useful references for the development of novel berry juices. In addition to microbiological safety, this study on equivalent processing allows direct efficacy and quality comparisons of a given juice pasteurized by the three nonthermal technologies under consideration.
