Food Engineering / Gıda Mühendisliği

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Institution Author: search.filters.institutionAuthor.Ertürk, Handan

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  • Conference Object
    Photoautotrophic and Heterotrophic Micropropagation of Sugarcane Shoots in Controlled Environments
    (2000) Ertürk, Handan; Walker, Paul N.
    We achieved successful growth and multiplication of sugarcane shoots in vitro under photoautotrophic conditions aseptically for three generations in our laboratory and proposed an experimental procedure for photoautotrophic micropropagation of sugarcane in controlled environment. The growth rate of photoautotrophic cultures in sugarless MS medium was found to be half of the growth rate of heterotrophic cultures in multiplication medium after a two-week period in the same environment. Contamination with algae in some vessels was recognized as a problem for septic photoautotrophic growth. Atrazine was found to be effective in controlling algae at a concentration of 10 ppm. But, atrazine in total was not considered to be a practical solution for the algae problem as it had a negative affect on the growth of shoots.
  • Article
    Citation - WoS: 7
    Citation - Scopus: 11
    Effects of Light, Carbon Dioxide, and Hormone Levels on Transformation To Photoautotrophy of Sugarcane Shoots in Micropropagation
    (American Society of Agricultural Engineers, 2000) Ertürk, Handan; Walker, Paul N.
    Sugarcane (Saccharum spp.) shoots were transferred from a heterotrophic micropropagation environment and cultured on sugar-free Murashige and Skoog (MS) salts medium in the controlled atmosphere of a growth chamber. The purpose was to achieve photoautotrophic shoot culture to be used for micropropagation. Effects on the shoots were tested for three factors: Carbon dioxide concentration, light level, and the hormone concentration of the growth medium. Factorial design was applied for the experiment such that all combinations of high and low factor levels were utilized, and the medium level of each factor level constituted the middle point of the design. All shoots were observed to become yellowish in color and lose vigor in the sugarless environment, although the successful treatments regained their color and vigor. Average dry weights of shoots per vessel were recorded at the end of two weeks as a quantitative measure of transformation of shoots to photoautotrophy. Light and CO2 levels were found to have statistically significant and positive effects. The negative effect of hormone concentration was insignificant.