Food Engineering / Gıda Mühendisliği
Permanent URI for this collectionhttps://hdl.handle.net/11147/12
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Data Paper Knockdown of Death Receptor 5 Antisense Long Noncoding Rna and Cisplatin Treatment Modulate Similar Macromolecular and Metabolic Changes in Hela Cells(TÜBİTAK - Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, 2022) Gürer, Dilek Cansu; Erdoğan Vatansever, İpek; Ceylan, Çağatay; Akgül, BünyaminBackground/aim: Despite great progress in complex gene regulatory mechanisms in the dynamic tumor microenvironment, the potential contribution of long noncoding RNAs (lncRNAs) to cancer cell metabolism is poorly understood. Death receptor 5 antisense (DR5-AS) is a cisplatin inducible lncRNA whose knockdown modulates cell morphology. However, its effect on cell metabolism is unknown. The aim of this study is to examine metabolic changes modulated by cisplatin and DR5-AS lncRNA in HeLa cells. Materials and methods: We used cisplatin as a universal cancer therapeutic drug to modulate metabolic changes in HeLa cervix cancer cells. We then examined the extent of metabolic changes by Fourier transform infrared spectroscopy (FTIR). We also performed transcriptomics analyses by generating new RNA-seq data with total RNAs isolated from cisplatin-treated HeLa cells. Then, we compared cisplatin-mediated transcriptomics and macromolecular changes with those mediated by DR5-AS knockdown. Results: Cisplatin treatment caused changes in the unsaturated fatty acid and lipid-to-protein ratios and the glycogen content. These observations in altered cellular metabolism were supported by transcriptomics analyses. FTIR spectroscopy analyses have revealed that DR5-AS knockdown causes a 20.9% elevation in the lipid/protein ratio and a 76.6% decrease in lipid peroxidation. Furthermore, we detected a 3.42% increase in the chain length of the aliphatic lipids, a higher content of RNA, and a lower amount of glycogen indicating relatively lower metabolic activity in the DR5-AS knockdown HeLa cells. Interestingly, we observed a similar gene expression pattern under cisplatin treatment and DR5-AS knockdown HeLa cells. Conclusion: These results suggest that DR5-AS lncRNA appears to account for a fraction of cisplatin-mediated macromolecular ametabolic changes in HeLa cervix cancer cells.Article Citation - WoS: 1Orta Nemli İncirlerde Depolama Sonrasında Pektin Metil Esteraz Aktivitesi Değişimi(Pamukkale Üniversitesi, 2015) Demirbüker Kavak, Dilek; Yemenicioğlu, AhmetOrta nemli meyveler, kurutulmuş meyvelerin rehidre edilmesiyle elde edilebilmektedirler. Kuru meyvelere kıyasla doğrudan tüketime daha uygun olan orta nemli meyveler, sanayide unlu mamüller, süt ürünleri, şekerlemeler kullanılabilmektedirler. Bu çalışmanın amacı orta nemli hale getirilmiş incirlerin 3 aylık depolama süresi sonucunda mikrobiyal stabilitelerinin ve dokuda yumuşamaya neden olan pektin metil esteraz enzimi (PME) aktivitelerinin kıyaslanmasıdır. Bu amaçla kuru incirler 30°C ve 80°C'de suyla %30 nem içeriğine ulaşana kadar rehidre edilmişlerdir. Rehidre edilmiş örnekler, 3 ay süreyle +4°C'de depolanmışlardır. Depolama süresi sonunda toplam canlı sayımı sonuçlarına göre 30°C kontrol örnekleri ile 80°C'de rehidre edilmiş örnekler arasında istatistiksel olarak anlamlı bir fark bulunmamıştır. Depolama süresi sonucunda PME aktivitesi, kontrol örnekleri için 24.1 ?mol COOH dk-1g-1 iken, 80°C'de rehidre edilmiş orta nemli incirlerde 17.4 ?mol COOH dk-1g-1bulunmuştur. Sonuç olarak incirleri orta nemli hale getirmek için 80°C'de gerçekleşen rehidrasyon işlemi, depolama sonrası mikrobiyal yükü belirgin şekilde etkilememesine karşın, yumuşamaya neden olan PME aktivitesini 30°C'de rehidre edilmiş kontrol örneklerine kıyasla %28 oranında azaltmaktadır.Article Kurutma Sıcaklığının Üzüm Çekirdeklerinin Toplam Fenolik Madde İçeriği ve Antioksidan Kapasitesi Üzerine Etkisi(Pamukkale Üniversitesi, 2015) Konuk, Dilara; Korel, FigenÜzüm çekirdeği, başta şarap olmak üzere meyve suyu ve pekmez ürünlerinin üretiminde açığa çıkan organik bir atık olup, biyoaktif bileşikler açısından oldukça zengin olması nedeniyle gıda formülasyonlarında değerlendirilmektedir. Bu çalışma, kurutma sıcaklığının üzüm çekirdeklerinde bulunan biyoaktif bileşikler üzerine etkisini incelemek amacıyla yapılmıştır ve çalışmada kabin tipi kurutma sisteminde sıcak hava ile üç farklı ortam sıcaklığında (40, 50 ve 60°C) kurutulan üzüm çekirdeklerinin toplam fenolik madde miktarı ve antioksidan aktivitesi belirlenmiştir. Taze üzüm çekirdekleri kurutulmuş örneklerle karşılaştırıldığında, kurutma işleminin toplam fenolik madde miktarında azalmaya neden olduğu ve kurutma sıcaklığı arttıkça toplam fenolik madde miktarının azaldığı görülmüştür. ABTS radikal indirgeme kapasitesi yöntemiyle belirlenen Troloks Eşdeğeri Antioksidan Kapasiteleri (TEAK) taze üzüm çekirdeklerinde daha yüksek değerlerde olup üç farklı sıcaklıkta kurutulan üzüm çekirdeklerinde ise birbirine yakın değerlerde bulunmuştur. Yapılan çalışma sonucunda üzüm çekirdeklerinin güçlü bir antioksidan kaynağı olduğu ve kurutma işlemi sonrasında da antioksidan özelliğini koruduğu görülmüştür. Ancak, fenolik bileşen kayıplarını azaltmak için kurutma işleminin düşük sıcaklıklarda yapılmasının avantajlı olduğu ortaya konulmuştur.Article Siyah Meyveli İncir Çeşitlerinin Kurutularak Değerlendirilmesi(Pamukkale Üniversitesi, 2015) Konak, Ramazan; Kösoğlu, İlknur; Tan, Nilgün; Kocataş, Hilmi; Yemenicioğlu, Ahmetİncir, yüksek oranda diyet lif, fenolik madde ve mineralleri içeren bir meyvedir. Ülkemizin sahip olduğu zengin incir mirası göz önüne alındığında antioksidan kapasitesi çok yüksek, renkli çeşitler mevcut olmasına rağmen bunların kurutularak değerlendirilme uygulamaları yok denecek kadar azdır. Bu çalışma ile İncir Araştırma Enstitüsü genetik kaynaklar parselinde bulunan siyah renkli çeşitlerden 9 tanesi ve standart kurutmalık çeşitler olan Sarılop ve Sarızeybek çeşitleri kullanılmıştır. Meyveler geleneksel yönteme göre kerevetler ile güneş altında kurutulup çeşitlerin kuruma kapasiteleri ve kuru meyve kaliteleri tespit edilmiştir. Standart kurutmalık çeşitlerin yanı sıra 1101, 1102 ve 1012 kod numaralı siyah renkli çeşitlerin kurutmaya uygun olduğu sonucuna varılmıştır.Article Citation - WoS: 4Citation - Scopus: 4The Volatile Compounds, Free Fatty Acid Composition and Microbiological Properties of Sepet Cheese Packaged With Different Modified Atmosphere Conditions(Kafkas Üniversitesi, 2017) Akpınar, Aslı; Yerlikaya, Oktay; Kınık, Özer; Kahraman, Cihan; Korel, Figen; Uysal, Harun RaşitThe objective of this present study was to investigate traditional sepet cheese samples which were packaged with different modified atmosphere conditions (MAP). The volatile compounds, free fatty acid composition and microbiological properties of Sepet cheeses were analyzed on 1., 45., 90. and 180. day of the storage period at +4°C. In packaking of cheese, three different modified atmosphere packaging conditions were tried. These cheeses were packaged into polystyrene packages that include 100% N2 (N), 80% N2 + 20% CO2 (NC), 100% CO2 (C). In accordance with volatile compounds of sepet cheese samples, it was shown that control cheese sample was different from MAP samples in terms of aroma fractions. MAP samples showed variability in preservation of aroma fractions. When fatty acid composition during the storage period was researched, it was found that there was a significant difference in all samples. When microbiological properties were investigated statistically, it was determined that there was not a significant difference in samples during storage. In general, it could be said that sepet cheeses that were packaged in different modified conditions protected their specialities better.Article Citation - WoS: 13Citation - Scopus: 14Evaluation of Orange Peel, an Industrial Waste, for the Production of Aspergillus Sojae Polygalacturonase Considering Both Morphology and Rheology Effects(TUBITAK, 2014) Gögüş, Nihan; Hakgüder Taze, Bengi; Demir, Hande; Tarı, Canan; Ünlütürk, Sevcan; Lahore, Marcelo FernandezOrange peel is an agroindustrial waste rich in pectin and known to be an inducer for pectinase production. The use of this low-cost substrate for the production of an industrially important enzyme, polygalacturonase (PG), can be an alternative way to turn this waste into a value-added product, contributing to the reduction of environmental waste disposal problems. Enzyme productions by fungal microorganisms are affected by environmental and nutritional factors, demanding the determination of optimum conditions for maximum enzyme production with the desired fungal morphology and broth rheology. Therefore, complex and additional carbon sources were optimized with respect to PG production by Aspergillus sojae using statistical approaches. Effect of pH, another significant parameter affecting the rheology and morphology of the strain, was investigated in the serial bioreactor system using the optimized medium composition. Highest PG enzyme yield and productivity together with the maximum PG enzyme production (93.48 U/mL) were obtained under uncontrolled pH conditions. Under these conditions, morphologically, pellet sizes exhibited a normal distribution ranging between 0.5-1.0 mm and 1.0-1.5 mm, and rheological measurements revealed that fermentation broths showed non-Newtonian flow. The low pH trend observed during the course of the fermentation was another important positive outcome for industrial fermentations, prone to contamination problems.Article Citation - WoS: 1Citation - Scopus: 1Prevalence of Salmonella in Edible Offal in Afyonkarahisar Province, Turkey(Kafkas Üniversitesi, 2012) Akkaya, Levent; Atabay, Halil İbrahim; Gök, Veli; Yaman, HilmiIn this study, the prevalence of Salmonella sp. was examined using a total of 205 edible bovine offal samples collected from different abattoirs (n=105) and butcheries (n=100) by immunomagnetic separation (IMS) method. The isolation rate of Salmonella was found to be 8.57%, 5.71% and 5.71% for the liver, kidney and tripe samples collected from the abattoir, respectively. Of the offal samples obtained from the butcheries, the detection rate of Salmonella sp. was 16% in the liver and 4% in the kidney, tripe and brain samples. Overall, it was found that 8.29% of all the offal samples obtained from the abattoirs and butcheries were determined to be contaminated with Salmonella sp. The results of this study shows that edible offal are cross-contaminated by Salmonella sp. at the abattoirs and retail sale points until they reach to the consumer. It is recommended that adequate hygienic and sanitary measures be taken in these kind of places in order to protect public health.Article Citation - WoS: 28Citation - Scopus: 32Optimization of the Process Parameters for the Utilization of Orange Peel To Produce Polygalacturonase by Solid-State Fermentation From an Aspergillus Sojae Mutant Strain(TUBITAK, 2012) Demir, Hande; Göğüş, Nihan; Tarı, Canan; Heerd, Doreen; Lahore, Marcelo FernandezThe effect of orange peel concentration, HCl concentration, incubation time and temperature, and inoculum size on the spore count and activity of polygalacturonase (PG) enzyme produced from Aspergillus sojae M3 by solidstate fermentation was screened using 2k factorial design. Orange peel and HCl concentrations and incubation time were significant factors affecting the responses. Optimum conditions favoring both PG and spore production from Aspergillus sojae M3 were determined as 2% orange peel and 50 mM HCl concentrations at 22 °C and 4.3 days of incubation. An overlay plot was constructed for use as a practical chart for production of high enzyme activity (>35.0 U/g substrate) and spore count (9.0 × 108 to 2.0 × 109 spore/mL) by superimposing the contours of PG activity and spore count responses. The accuracy and reliability of the constructed models on the responses was validated with the maximum calculated error rate between the predicted and actual activities at 14.1% and 22.4%, respectively. © TÜBİTAK.