Food Engineering / Gıda Mühendisliği
Permanent URI for this collectionhttps://hdl.handle.net/11147/12
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Review Citation - WoS: 25Citation - Scopus: 27Bacillus Cereus: a Review of “fried Rice Syndrome” Causative Agents(Academic Press, 2023) Leong, Sui Sien; King, Jie Hung; Korel, Figen“Fried rice syndrome” originated from the first exposure to a fried rice dish contaminated with Bacillus cereus. This review compiles available data on the prevalence of B. cereus outbreak cases that occurred between 1984 and 2019. The outcome of B. cereus illness varies dramatically depending on the pathogenic strain encounter and the host's immune system. B. cereus causes a self-limiting, diarrheal illness caused by heat-resistant enterotoxin proteins, and an emetic illness caused by the deadly toxin named cereulide. The toxins together with their extrinsic factors are discussed. The possibility of more contamination of B. cereus in protein-rich food has also been shown. Therefore, the aim of this review is to summarize the available data, focusing mainly on B. cereus physiology as the causative agent for “fried rice syndrome.” This review emphasizes the prevalence of B. cereus in starchy food contamination and outbreak cases reported, the virulence of both enterotoxins and emetic toxins produced, and the possibility of contaminated in protein-rich food. The impact of emetic or enterotoxin-producing B. cereus on public health cannot be neglected. Thus, it is essential to constantly monitor for B. cereus contamination during food handling and hygiene practices for food product preparation. © 2023 Elsevier LtdArticle Citation - WoS: 22Citation - Scopus: 33Uv-C Irradiation of Freshly Squeezed Grape Juice and Modeling Inactivation Kinetics(John Wiley and Sons Inc., 2014) Ünlütürk, Sevcan; Atılgan, Mehmet ReşatUV inactivation kinetics of freshly squeezed turbid white grape juice (FSTGJ) treated with an annular flow UV reactor by applying UV dosages ranging from 0 to 116.7J/mL, at three different flow rates (0.90, 1.75 and 3.70mL/s), were modeled by using log-linear, Weibull, Hom and modified Chick-Watson models. FSTGJ was circulated five times in the UV system, i.e., UV exposure time was 20.33min during processing. The populations of Escherichia coli K-12, lactic acid bacteria (LAB) and foodborne yeasts were reduced by 3.759, 4.133 and 1.604log cfu/mL, respectively, after exposure to UV dosage of 116.7J/mL at the lowest flow rate. The inactivation kinetics of foodborne yeasts were best described by the modified Chick-Watson model, with the least root mean squared error (RMSE=0.001, R2=0.999). Besides, the inactivation kinetics of E.coli K-12 and LAB were best fitted by Weibull model (R2=0.999). Additionally, when the UV exposure time was increased up to 32.5min (i.e., eight cycles), UV-C treatment of FSTGJ resulted in 5.341log cfu/mL reduction in E.coli K-12, which meets the Food and Drug Administration requirement of a 5log reduction of microorganisms in fruit juices. Practical Applications Consumer demand for high-quality fruit juice with fresh-like characteristics has markedly expanded in recent years. UV-C irradiation is a nonthermal method and allows the processing of fruit juices with a minimal or no changes in flavor, essential nutrients and vitamins. Although thermal pasteurization is the most convenient way of increasing the shelf life of fruit juices, it causes a "cook taste" in grape juice. So, in this study, the application of UV-C irradiation to process grape juice was investigated. The shape of the microbial inactivation curve is sigmoidal in UV treatment. Therefore, different kinetic models (e.g., log-linear, Weibull, Hom and modified Chick-Watson) are applied to describe the inactivation kinetics of Escherichia coli K-12, lactic acid bacteria and foodborne yeasts. Kinetic parameters (e.g., k and D) and models can be used for the development of UV-C irradiation process to ensure microbial safety in juice products.Article Citation - WoS: 7Citation - Scopus: 10Quality of Flavored Yogurt Containing Added Coffee and Sugar(Hindawi Publishing Corporation, 2007) Tan, Gürdal; Korel, FigenYogurts flavored with instant coffee (0.5, 0.7 and 0.9%) and sweetened with sugar (4 or 5%) and a control yogurt (no coffee or sugar) were evaluated for chemical, physical, microbiological and sensory properties. The added ingredients generally had no effect on the chemical, physical and microbiological quality of yogurts at day 1 after preparation as compared to the control. During 15 days at 5-7C, pH and lactic acid bacteria counts decreased (P < 0.05) and titratable acidity increased (P < 0.05) in all samples. Yogurts with 0.5% coffee flavoring and 4 and 5% sugar met Turkish Institute Standards for yogurt sensory quality when evaluated by a trained 10-member panel. Yogurt flavored with 0.5% coffee and containing 5% sugar had most attributes rated in the "like" category by 50% or more of 51 consumer panelists.Article Citation - WoS: 21Citation - Scopus: 24Isolation and Characterization of Bacillus Thuringiensis Strains From Different Grain Habitats in Turkey(Springer Verlag, 2005) Apaydın, Özgür; Yenidünya, Ali Fazıl; Harsa, Hayriye Şebnem; Güneş, HaticeBacillus thuringiensis (Bt) is a gram-positive, spore-forming bacterium and it produces insecticidal crystal (cry) proteins during sporulation. Because the genetic diversity and toxic potential of Bt strains differ from region to region, strains have been collected and characterized all over the world. The aim of this study is to isolate Bt strains in grain-related habitats in Turkey and to characterize them on the basis of crystal morphology, cry gene content, and chromosomal and plasmid DNA profiles. Four approaches were taken analysis with phase contrast (PC) microscopy, polymerase chain reaction (PCR), pulsed field gel electrophoresis (PFGE) and plasmid isolation. Ninety-six samples were collected from Central Anatolia and the Aegean region. Bt was isolated from 61 of 96 samples (63.5) and 500 Bt-like colonies were obtained. One hundred and sixty three of the colonies were identified as Bt based on cry protein formation using PC microscopy. Among the examined colonies, the overall proportion identified (as Bt index) was 0.33. We found that 103 isolates were positive for the five different cry genes (cry1, cry2, cry3, cry4 and cry9) examined with PCR. In addition, plasmid profiling of 37 cry gene-positive isolates indicated that the 15 kb plasmid band was present in all isolates; however, 11 of 37 isolates had more than one plasmid band at different sizes. Finally, chromosomal DNA profiling by PFGE gave rise to different DNA patterns for isolates containing the same cry gene which suggests a high level of diversity among the Bt strains isolated.