Food Engineering / Gıda Mühendisliği

Permanent URI for this collectionhttps://hdl.handle.net/11147/12

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Subject: search.filters.subject.EnzymesWoS Q: search.filters.wosQuartile.Q2

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  • Article
    Citation - WoS: 76
    Citation - Scopus: 90
    Optimization of a Growth Medium Using a Statistical Approach for the Production of an Alkaline Protease From a Newly Isolated Bacillus Sp. L21
    (Elsevier Ltd., 2006) Tarı, Canan; Gençkal, Hande; Tokatlı, Figen
    Bacillus sp. L21 was isolated from the by-products of a leather factory (located in Izmir, Turkey) working under extreme alkaline conditions. Its phenotypic and genotypic identifications were completed, and determined as a potential alkaline protease producer. After screening various elements, carbon and nitrogen sources, soybean meal, maltose50, tween80 and the initial pH conditions were chosen as main factors to be used in the experimental design and response surface methodology (RSM) for the optimization of a low cost enzyme producing media for potential use on an industrial scale. The optimized values obtained by the statistical analysis showed that soybean meal at 3.0 g/l, maltose50 between the ranges of 30 and 40 g/l, tween80 at 0.35 g/l and an initial pH of 8.0 gives maximum protease activity.
  • Article
    Citation - WoS: 33
    Citation - Scopus: 37
    Relationship Between Morphology, Rheology and Polygalacturonase Production by Aspergillus Sojae Atcc 20235 in Submerged Cultures
    (Elsevier Ltd., 2006) Göğüş, Nihan; Tarı, Canan; Öncü, Şelale; Ünlütürk, Sevcan; Tokatlı, Figen
    A full factorial statistical design, with the factors of, two taxonomically different strains, seven types of seed culture formulations (slants) and two types of fermentation media were used to investigate the effect of these parameters on the morphology and polygalacturonase production. The rheology of the final fermentation medium was analyzed and appropriate mathematical model was applied to calculate suspension viscosity. It was found that most fermentation broths showed non-Newtonian flow behavior. According to statistical analysis, factors of strain types and fermentation media and the interaction between them were found significant on the enzyme activity. The effect of seed culture formulations (slants) were found insignificant at the significance level of 1%. Interaction of slants with strain types and fermentation media were also found insignificant. Considering the morphology of the final culture, Aspergillus sojae with the desired pellet morphology in a complex media, inoculated with a seed culture prepared from molasses resulted in maximum polygalacturonase enzyme activity (0.2 U/ml) and lowest suspension viscosity with a broth rheology close to Newtonian flow behavior.