Food Engineering / Gıda Mühendisliği
Permanent URI for this collectionhttps://hdl.handle.net/11147/12
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Conference Object Potential Application of Natural Phenolic Antimicrobials and Edible Film Technology Against Bacterial Plant Pathogens(International Society for Horticultural Science, 2020) Alkan, Derya; Yemenicioğlu, AhmetIn this study, antimicrobial edible film technology used frequently for inhibition of human pathogens in processed food was employed as a postharvest bio-based preservation method to prevent bacterial spoilage of fresh fruits and vegetables. Antimicrobial edible zein films were developed by incorporation of pure phenolic acids (PAs) such as gallic acid (GA), cinnamic acid (CA), vanillic acid (VA); essential oils (EOs) such as carvacrol (CAR), thymol (THY), eugenol (EUG) and citral (CIT) and phenolic extract (PE) from clove. In order to test synergetic effect of PAs, different amounts of PAs were added into film solution to obtain films containing mixture of GA and CA, GA and VA, CA and VA at different concentrations. The antimicrobial performances of edible films were tested on selected plant pathogenic bacteria including Pseudomonas syringae, Erwinia amylovora, Xanthomonas vesicatoria and Erwinia carotovora. The overall results of antimicrobial tests showed that films containing PAs between 1 and 4 mg cm-2 inhibited all pathogens while EOs at concentrations between 2 and 4 mg cm-2 and clove extract between 4 and 8 mg cm-2 were found effective against pathogens except P. syringae. Morover, the incorporation of combination of different PA showed greater inhibitory activity on plant pathogens than incorporation of a single PA into films. The edible zein coatings have already been applied for coating of fresh fruits and vegetables at the postharvest stage to suppress their respiration rate and prolong their refrigerated storage. This work clearly showed the benefit of incorporating a single or mixture of antimicrobial phenolic compounds, EOs and PEs in edible zein films against bacterial plant pathogens. The developed films might be employed against plant pathogens by coating fresh fruits and vegetables, seeds, seedlings, tree stems, soil as well as agricultural tools and constructions without using toxic chemicals. © 2020 International Society for Horticultural Science. All rights reserved.Article Citation - WoS: 70Citation - Scopus: 89Potential Application of Natural Phenolic Antimicrobials and Edible Film Technology Against Bacterial Plant Pathogens(Elsevier Ltd., 2016) Alkan, Derya; Yemenicioğlu, AhmetThe aim of the present study is to use antimicrobial edible film technology and natural phenolic antimicrobials for inhibition of major bacterial plant pathogens such as Erwinia amylovora, Erwinia carotovora, Xanthomonas vesicatoria and Pseudomonas syringae. For this purpose phenolic acids (PAs) (gallic (GA), vanillic (VA), cinnamic acids (CA)), essential oils (EOs) (carvacrol (CAR), thymol (THY), eugenol (EUG) citral (CIT)), phenolic extracts (PEs) from clove (CE), oregano (OE), artichoke stem (ASE) and walnut shells (WSE) were evaluated as antimicrobial zein film components. Films containing PAs between 1 and 4 mg/cm2 inhibited all pathogens while EOs between 1 and 4 mg/cm2 and CE between 4 and 8 mg/cm2 inhibited pathogens except P. syringae. The most potent films were obtained by using GA against E. amylovora and P. syringae, VA against E. carotovora, and CA, THY or CAR against X. vesicatoria. The addition of phenolic compounds into films increased the porosity of films. The phenolic containing films also become more flexible and lost their brittleness. This study is important in that it prepared the basis of using edible antimicrobial coatings in outdoor applications on infected tree stems, soil surfaces and agronomy tools or in classical fruit and seedling coating applications to control bacterial contamination or spoilage.
