Food Engineering / Gıda Mühendisliği
Permanent URI for this collectionhttps://hdl.handle.net/11147/12
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Article Citation - WoS: 16Citation - Scopus: 18Microencapsulation of a Potential Probiotic Lactiplantibacillus Pentosus and Its Impregnation Onto Table Olives(Elsevier, 2022) Elvan, Menşure; Baysal, Ayşe Handan; Harsa, Hayriye ŞebnemThis study aimed to demonstrate some probiotic properties of Lactiplantibacillus pentosus NRRL B-227 and impregnate the strain onto the table olive surfaces. In this respect, antioxidative ability, antibiotic resistance, and survivability after simulated digestion tests were carried out. Microencapsulation was performed using xylan and whey protein concentrate (WPC) using the water-in-oil emulsion technique to maintain cell viability. A vacuum impregnation process was performed to coat olive surfaces with L. pentosus. This strain demonstrated 71.6% DPPH radical scavenging activity and exhibited paramount resistance to antibiotics. The viable cell count of microencapsulated L. pentosus was found as 8 log CFU/g after 72 weeks of storage. After exposure to the simulated oral phase, 1-log reduction was detected, and gastric phase conditions led to a 3-log reduction of viability of microencapsulated as well as non-microencapsulated cells. The viability of microencapsulated L. pentosus on the surface of olives was also evaluated for one-month, and viable cell count was ≥6 log CFU/g. In the light of these findings, L. pentosus with antibiotic and digestion fluids resistant and antioxidant properties were successfully microencapsulated within xylan-WPC complex. Table olives can be considered as a suitable carrier for beneficial microorganisms that satisfies with the expectations of regulations for functional foods.Article Citation - WoS: 15Citation - Scopus: 17Effect of Different Microencapsulating Materials on the Viability of S. Thermophilus Ccm4757 Incorporated Into Dark and Milk Chocolates(Elsevier Ltd., 2021) Öztürk, Burcu; Elvan, Menşure; Özer, Merve; Tellioğlu Harsa, ŞebnemThis study aimed to evaluate the viability and bioaccessibility of Streptococcus thermophilus CCM4757 strain supplemented in dark and milk chocolates during storage period and pass through simulated in vitro gastro-intestinal tract. Microencapsulated and non-microencapsulated S. thermophilus CCM4757 strain added into the chocolates. Emulsion technique was used to microencapsulate cells with various biopolymers; carboxymethylcellulose, pectin, gum arabic, and cellobiose. The microencapsulated S. thermophilus with these coating materials was found to be viable higher than 9 log CFU/g up to 180 days of storage at 4 °C. Microbiological, physicochemical, and sensorial attributes of the chocolates containing microencapsulated and non-microencapsulated S. thermophilus CCM4757 were analyzed. The microencapsulated S. thermophilus showed a good survivability in milk (7.12 log CFU/g) and dark (6.90 log CFU/g) chocolate samples during 180-day storage at 4 °C. Supplementation of S. thermophilus did not affect significantly (P > 0.05) the sensory attributes of the chocolates. The results showed that S. thermophilus CCM4757 exhibited good cell survivability higher than 85% in chocolates under simulated gastro-intestinal fluids. S. thermophilus supplementation into the chocolate protected the viability of cells and did not affect the sensorial characteristics and moisture content of chocolates. The present study demonstrated that the dark and milk chocolates could be used as an important matrix to carry probiotics. © 2021 Elsevier Ltd