Food Engineering / Gıda Mühendisliği
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Article Citation - WoS: 42Citation - Scopus: 53Effects of Ultraviolet-Light Emitting Diodes (uv-Leds) on Microbial Inactivation and Quality Attributes of Mixed Beverage Made From Blend of Carrot, Carob, Ginger, Grape and Lemon Juice(Elsevier Ltd., 2021) Baykuş, Gökçen; Pelvan Akgün, Merve; Ünlütürk, SevcanEfficacy of ultraviolet light-emitting diodes (UV-LEDs) with peak and coupled emissions at 280, 365 and 280/365 nm on inactivation of E. coli K12 in mixed beverage (MB) was investigated. MB comprised of 31.6% carrot, 44.3% carob, 8.7% grape, 10.2% ginger, and 5.2% lemon juice. The impact of UV-LEDs on some physicochemical and phytochemical properties of MB was compared to that of heat treatment (70 °C, 120 s). While, UV-LED irradiation using coupled 280/365 nm for 40 min resulted in the highest inactivation of E. coli K12 (>4 log) out of tested wavelengths, the number of mesophilic bacteria (TAC), and yeast and molds (YM) in mixed beverage were reduced by 2.59 log CFU/mL (from 5.69 log CFU/mL of initial load), and 0.17 log CFU/mL (from 3.28 log CFU/mL of the initial load), respectively. Although, the color parameters slightly changed after irradiation, the color of MB did not show visual difference (?E = 0.94) compared to untreated samples. UV-LED treatment caused a significant increase in total phenolic compound (1.75-fold) and antioxidant capacity (4.60 fold) compared to heat-treated samples (p < 0.05). UV-LED treatment caused a decrease in carotenoid content (71.3%) lower than that of heat-treated samples (88.9%), indicating that UV-LED irradiation preserved the total carotenoid content better than the heat treatment. Industrial relevance: Light-emitting diodes (LEDs) are new sources of ultraviolet light utilized for non-thermal processing of foods. In this study, a static bench top unit was designed to investigate the efficacy of UV-LEDs with different treatment times and peak emissions by considering the inactivation of E. coli K12 in newly formulated mixed drink (MB). UV-LED irradiation of MB using coupled 280/365 nm for 40 min provided the highest microbial inactivation and preserved bioactive compounds better than the heat treatment. It can be proposed as an effective method for the processing of fruit juices which is rich in bioactive constituents. © 2020 Elsevier LtdArticle Citation - WoS: 22Citation - Scopus: 28Effect of Postharvest Uv-C Treatment on the Microbial Quality of ‘şalak’ Apricot(Elsevier Ltd., 2018) Hakgüder Taze, Bengi; Ünlütürk, SevcanInactivation of natural flora found on cv. ‘Şalak’ apricot surfaces was investigated using a newly designed UV-C treatment system equipped with four UV lamps and a rotating roller bearing. Multiple Point Source Summation (MPSS) Model was used to estimate UV light intensity field in this processing chamber. Although MPSS model over-predicted the UV intensity data, light intensity profile showed a good agreement with the radiometric measurements. UV-C treatment of apricots resulted in 3-log reduction in the number of total mesophilic aerobic bacteria (TAPC) at 32.3 W m−2 of average UV light intensity for 16 min at 31.01 kJ m−2 of UV dose whereas 2.38-log reduction was achieved for yeast and mould count (YMC) after 4 min of UV exposure at 7.75 kJ m−2 of UV dose. On the other hand, complete inactivation of coliform bacteria was observed after all treatments. Statistical analysis indicated that further extension of the exposure time did not cause any significant effect on the inactivation of natural flora of apricot surfaces. Additionally, the inactivation data obtained for TAPC and YMC were modelled by using log-linear regression, Biphasic model, log-linear + tail, Weibull model, Weibull + tail, double Weibull and biphasic + shoulder models. The inactivation kinetics of TAPC and YMC were best described by log linear + tail model with the smallest root mean squared error (RMSE) and the highest regression coefficient (R2 > 0.90). These results suggest that UV-C treatment can be applied to control the natural flora on cv. ‘Şalak’ apricots. Use of a rotating roller bearing is highly appreciated in UV-C systems to provide equal radiation among fruit surfaces.Article Citation - WoS: 81Citation - Scopus: 115Effects of Ultraviolet Light Emitting Diodes (leds) on Microbial and Enzyme Inactivation of Apple Juice(Elsevier Ltd., 2017) Pelvan Akgün, Merve; Ünlütürk, SevcanIn this study, the effects of Ultraviolet light-emitting diodes (UV-LEDs) on the inactivation of E. coli K12 (ATCC 25253), an indicator organism of E. coli O157:H7, and polyphneoloxidase (PPO) in cloudy apple juice (CAJ) were investigated. The clear (AJ) and cloudy apple juice were exposed to UV rays for 40 min by using a UV device composed of four UV-LEDs with peak emissions at 254 and 280 nm and coupled emissions as follows: 254/365, 254/405, 280/365, 280/405 and 254/280/365/405 nm. UV-LEDs at 254 nm achieved 1.6 ± 0.1 log10 CFU/mL inactivation of E. coli K12 at UV dose of 707.2 mJ/cm2. The highest inactivation of E. coli K12 (2.0 ± 0.1 log10 CFU/mL and 2.0 ± 0.4 log10 CFU/mL) was achieved when the cloudy apple juice was treated with both 280 nm and 280/365 nm UV-LEDs. For clear apple juice the highest inactivation 4.4 log10 CFU/mL obtained for E. coli K12 was achieved using 4 lamps emitting light at 280 nm for 40 min exposure time. For the same treatment time, the experiments using a combination of lamps emitting light at 280 and 365 nm (2lamp/2lamp) were resulted in 3.9 ± 0.2 log10 CFU/mL reductions. UV-A and UV-C rays in combination showed a better inactivation effect on PPO than UV-C rays used separately. Residual activity of PPO in CAJ was reduced to 32.58% when treated with UV-LED in combination of UV-C (280 nm) and UV-A (365 nm) rays. Additionally, the total color change (ΔE) of CAJ subjected to combined UV-LED irradiation at 280/365 nm was the lowest compared to other studied processing conditions. This study provides key implications for the future application of UV-LEDs to fruit juice pasteurization.Article Citation - WoS: 82Citation - Scopus: 103Effect of Uv-C Irradiation and Heat Treatment on the Shelf Life Stability of a Lemon-Melon Juice Blend: Multivariate Statistical Approach(Elsevier Ltd., 2015) Kaya, Zehra; Yıldız, Semanur; Ünlütürk, SevcanHeat treatment and UV-C irradiation of lemon and melon juice (LMJ) blends were comparatively evaluated by examining their impact on E. coli K12 (ATCC 25253) and their physicochemical properties, i.e., total soluble solids (TSS), pH, titratable acidity (TA), color, turbidity and absorbance coefficient, both immediately after processing and during 30 days of refrigerated storage. The newly formulated LMJ blend containing 12% (v/v) lemon juice (pH 3.92 ± 0.01) scored the highest in the consumer acceptance test. Upon UV-C irradiation (2.461 J/mL) and heat treatment (72 °C, 71 s), the E. coli K12 population in LMJ blend was reduced by > 6 log10 CFU/mL. Principal component analysis (PCA) and hierarchical cluster analyses (HCA) showed a clear discrimination among the physicochemical properties of the control and the UV-C and heat-treated LMJ blends during storage, suggesting that UV-C irradiation has a comparable effect on microbial stability at 4 °C and better quality preservation performance than heat treatment. Industrial relevance Melon juice has many beneficial health effects. It has high sugar content, pH (5.6-6.0) and a fairly short shelf life. Therefore, pasteurization is required. But the thermal pasteurization has some undesired effects on the juice quality. Consumer demands for high quality fruit juice with fresh-like characteristics has markedly expanded in recent years. In this study, an alternative lemon-melon juice (LMJ) blend formulation was developed, and pasteurized using both UV-C irradiation and mild heat treatment. The shelf life stability of pasteurized LMJ blends was assessed by means of principal component analysis and hierarchical cluster analysis. The shelf life of LMJ blends treated by both methods was increased from 2 days to 30 days. The multivariate data analysis was successfully applied as a tool for an overall evaluation of the shelf-life of the product. UV-C irradiation has a comparable effect on microbial stability at 4 °C and better quality preservation performance than heat treatment for obtaining both shelf-stable and fresh-like LMJ blends. This would be a major advantage in processing of nutritious juice products.Article Citation - WoS: 76Citation - Scopus: 89Uv-C Light Inactivation and Modeling Kinetics of Alicyclobacillus Acidoterrestris Spores in White Grape and Apple Juices(Elsevier Ltd., 2013) Baysal, Ayşe Handan; Molva, Çelenk; Ünlütürk, SevcanIn the present study, the effect of short wave ultraviolet light (UV-C) on the inactivation of Alicyclobacillus acidoterrestris DSM 3922 spores in commercial pasteurized white grape and apple juices was investigated. The inactivation of A. acidoterrestris spores in juices was examined by evaluating the effects of UV light intensity (1.31, 0.71 and 0.38mW/cm2) and exposure time (0, 3, 5, 7, 10, 12 and 15min) at constant depth (0.15cm). The best reduction (5.5-log) was achieved in grape juice when the UV intensity was 1.31mW/cm2. The maximum inactivation was approximately 2-log CFU/mL in apple juice under the same conditions. The results showed that first-order kinetics were not suitable for the estimation of spore inactivation in grape juice treated with UV-light. Since tailing was observed in the survival curves, the log-linear plus tail and Weibull models were compared. The results showed that the log-linear plus tail model was satisfactorily fitted to estimate the reductions. As a non-thermal technology, UV-C treatment could be an alternative to thermal treatment for grape juices or combined with other preservation methods for the pasteurization of apple juice.Article Citation - WoS: 87Citation - Scopus: 99Modeling Inactivation Kinetics of Liquid Egg White Exposed To Uv-C Irradiation(Elsevier Ltd., 2010) Ünlütürk, Sevcan; Atılgan, Mehmet Reşat; Baysal, Ayşe Handan; Ünlütürk, Mehmet S.The efficiency of UV-C irradiation as a non-thermal pasteurization process for liquid egg white (LEW) was investigated. LEW inoculated with Escherichia coli K-12 (ATCC 25253), pathogenic strain of Escherichia coli O157:H7 (NCTC12900) and Listeria innocua (NRRL B33314) were treated with UV light using a bench top collimated beam apparatus. Inoculated LEW samples were exposed to UV-C irradiation of known UV intensity of 1.314mW/cm2 and sample depth of 0.153cm for 0, 3 5, 7, 10, 13, 17 and 20min. The populations of E. coli K-12, E. coli O157:H7 and L. innocua were reduced after 20min of exposure by 0.896, 1.403 and 0.960logCFU respectively. Additionally, the inactivation data obtained for each strain suspended in LEW was correlated by using Weibull (2 parameter), Log-Linear (1 parameter), Hom (2 parameter) and modified Chick Watson (2 parameter) models. The inactivation kinetics of E. coli K-12, E. coli O157:H7 and L. innocua were best described by modified Chick Watson model with the smallest root mean squared error (RMSE) (R2≥0.92). © 2010 Elsevier B.V.Letter Rebuttal: Response To Regarding Letter To Editor "on the Modeling of Inactivation Kinetics by Uv Irradiation"(Elsevier Ltd., 2010) Ünlütürk, SevcanDr. Buzrul has commented on the mathematical modeling part of the article of Unluturk et al. (2010) which has recently been published online in the International Journal of Food Microbiology. He criticised the parameters k, β and h obtained from Log linear model, Weibull and Hom models, respectively.Article Citation - WoS: 124Citation - Scopus: 144Use of Uv-C Radiation as a Non-Thermal Process for Liquid Egg Products (lep)(Elsevier Ltd., 2008) Ünlütürk, Sevcan; Atılgan, Mehmet Reşat; Baysal, Ayşe Handan; Tarı, CananThe efficacy of short wave ultraviolet light (UV-C) as a non-thermal process for liquid egg products (LEP) was investigated. Non-pathogenic Escherichia coli strain (ATCC 8739), which shows lower sensitivity to UV-C light than E. coli O157:H7 and Salmonella typhimurium, was chosen as a target microorganism. The inactivation of UV resistant strain of E. coli in LEP was examined by evaluating the effects of depth of liquid food medium (0.5, 0.3 and 0.153 cm), UV light intensity (1.314, 0.709 and 0.383 mW/cm2) and exposure time (0, 5, 10, and 20 min) by using a collimated beam apparatus. The best reduction (>2-log) was achieved in liquid egg white (LEW) when the fluid depth and UV intensity were 0.153 cm and 1.314 mW/cm2, respectively. Maximum inactivation was 0.675-log CFU/ml in liquid egg yolk (LEY) and 0.316-log CFU/ml in liquid whole egg (LWE) at the same conditions. The kinetics of UV inactivation of E. coli in LEP was nonlinear. Our results emphasize that UV-C radiation can be used as a pre-treatment process or combined with mild heat treatment to reduce the adverse effects of thermal pasteurization of LEP.Article Citation - WoS: 33Citation - Scopus: 37Relationship Between Morphology, Rheology and Polygalacturonase Production by Aspergillus Sojae Atcc 20235 in Submerged Cultures(Elsevier Ltd., 2006) Göğüş, Nihan; Tarı, Canan; Öncü, Şelale; Ünlütürk, Sevcan; Tokatlı, FigenA full factorial statistical design, with the factors of, two taxonomically different strains, seven types of seed culture formulations (slants) and two types of fermentation media were used to investigate the effect of these parameters on the morphology and polygalacturonase production. The rheology of the final fermentation medium was analyzed and appropriate mathematical model was applied to calculate suspension viscosity. It was found that most fermentation broths showed non-Newtonian flow behavior. According to statistical analysis, factors of strain types and fermentation media and the interaction between them were found significant on the enzyme activity. The effect of seed culture formulations (slants) were found insignificant at the significance level of 1%. Interaction of slants with strain types and fermentation media were also found insignificant. Considering the morphology of the final culture, Aspergillus sojae with the desired pellet morphology in a complex media, inoculated with a seed culture prepared from molasses resulted in maximum polygalacturonase enzyme activity (0.2 U/ml) and lowest suspension viscosity with a broth rheology close to Newtonian flow behavior.